搖篩 的英文怎麼說

中文拼音 [yáoshāi]
搖篩 英文
bull shaker
  • 搖 : Ⅰ動詞（搖擺; 使物體來回地動） shake; wave; rock; turn Ⅱ名詞（姓氏） a surname
  • 篩 : 名詞[書面語] （植物名） sedge
  1. Through screening a lot of mutants with the method of transparent zones and culture filtrate, the best four were obtained with high - yield of stable phb depolymerase, named as 02, 04, 09 and 14

    以青黴（ penicillium . sp ） ds9701為出發菌株，通過紫外線誘變分生孢子，採用透明圈初篩和搖瓶培養復篩的方法，獲得4個能穩定遺傳的phb解聚酶高產菌株。
  2. Size jigging screen

    分級搖動篩
  3. When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum

    莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。
  4. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  5. There was freckled places on the ground where the light sifted down through the leaves, and the freckled places swapped about a little, showing there was a little breeze up there

    有些地方，陽光透過樹葉，往下篩落，留下了地上幾處斑斑點點亮色。每當這些地方亮色搖曳，便知道有微風吹拂過。
  6. Streptomyces tenebrarius a04, the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper. after treatment of spore suspension and mycelia shivered by supersonic with mutagen, combined with the application of screening models, some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23, a2 - 30, asm6 and al - 16 were obtained

    本文以尼拉黴素單一組分?安普黴素產生菌s . tenebrariusa04 （發酵單位為2847u ml ）為出發菌株，通過對單孢子懸液和超聲波破碎菌體的誘變處理並復合篩選模型，獲得了遺傳特性穩定的單組分高產菌株： a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ，搖瓶發酵單位達4800 - 5200u ml 。
  7. In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method

    為研究外生菌根真菌本身對重金屬污染的耐性，比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法，以真菌生物量大小和分離難易程度為主要指標，篩選出液體靜置方法為最優方法。
  8. Study on screening a high - productive strain of dextranase and its basic conditions of flask fermentation

    葡聚糖酶高產菌株的誘變篩選及其搖瓶發酵條件的初步研究
  9. The jewels are scooped up from the ground along with dirt, grass, leaves, sticks and stones

    以篩子和搖晃機篩過這一大堆東西，留下不含雜質的堅果，最後用機器依照大小選別。
  10. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。
  11. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下： 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板培養基從土樣中篩選出102株酸性植酸酶產生菌，從中挑選出透明圈較大的菌株32株，經分離純化後分別進行搖瓶復篩， 28 、 220r / min發酵5天後，在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活，結果發現有3株菌產酶活性較高且產酶性能較為穩定。
  12. Gs115 strain with high copy inserts is obtained by 1500 mg l zeocin selection, which, in the optimized condition, express as high as 100 mg l target protein in flask fermentation

    使用濃度高達1500 g ml的zeocin篩選得到高拷貝插入的gs115 - h菌株，經過優化搖瓶表達條件表達量達到100 mg l 。
  13. Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5

    Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。
  14. One highly productive and genetically stable recombinant strain named e - 22, which produced phytase with 143958. 3u / ml under the condition of flask cultivation, was selected through further screening. the phytase activity of e - 22 was 341. 13 times as high as that of the original strain ( 422u / ml )

    經搖瓶復篩后得到l株產酶活性為143958 . 3uzml發酵液，並且具有良好遺傳穩定性的高產工程菌株（ e22 ） ，其產酶活性是出發菌株酶活性（ 422u / ml ）的341
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