放射電泳 的英文怎麼說
中文拼音 [fàngshèdiànyǒng]
放射電泳
英文
radioelectrophoresis-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。It went down quickly in turn, at 15min, it reached the lowest level, but it still be 1. 36 time s of the control, which was much higher than that of the control, then kept rising. the change of aba content shows a coincidence with what described above
通過電泳和放射性標記發現低溫脅迫導致甘藍葉片內迅速出現一種分子量為22 . 9kd的磷酸化蛋白質，這說明甘藍在低溫脅迫條件下的信號傳導過程仍然依賴于磷酸不扮去磷酸化過程，這種磷酸化蛋白非常有可能是該信號傳遞過程中的關鍵物質。
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