整合質粒 的英文怎麼說

中文拼音 [zhěnggězhílì]
整合質粒 英文
integrated plasmid
  • 整 : Ⅰ形容詞1 （全部在內; 完整） whole; all; complete 2 （整齊） neat; tidy; orderly Ⅱ動詞1 （整理; 整...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 整合 : commensuration
  1. At scinto 6 uranium occurs in much altered dolerite below the unconformity.

    「辛托6」的鈾礦體產于不整合面之下變質甚深的粒玄巖中。
  2. At scinto 6 uranium occurs in much altered dolerite below the unconformity

    「辛托6 」的鈾礦體產于不整合面之下變質甚深的粒玄巖中。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. However, in the late phase of the growth, the strain hv grew a little faster than e. colihms174. hv ( ptz101 ) was constructed by the plasmid containing the phb operon and the parde gene tansformed into the strain hv

    將帶有phb操縱子和質粒穩定分配基因parde的質粒ptz101引入vhb整合菌中，構建成為產phb的vhb整合菌hv （ ptz101 ） 。
  5. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  6. So some methods suitable to large plasmid extraction, including lysis by sds and a method from a literature, were used to try to extract the large plasmid from the strain cell. the lysis reactions in these two methods are gentle, so the large plasmid cannot be injured in the lysis process, opposite to lysis by alkali. it would be helpful to keep the integrality of the large plasmid during the extraction

    因此我們採用了適合於大質粒提取的sds法，和文獻中應用於硝基苯降解性質粒的提取方法，來嘗試對菌株細胞進行質粒提取，這兩種方法裂解反應溫和，不會像堿裂解法那樣，在裂解過程中損壞質粒，可以實現質粒提取的完整性。
  7. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  8. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  9. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  10. The abuses of antibiotics in medicine and livestock exposed to environmental bacteria lead to a large - scale dissemination of antibiotic - resistance bacteria in aquatic environment under selective pressure and the resistant organism could transfer resistance genes across the genus and species by plasmid and integron, antibiotic resistance microbes are common in aquatic environment and the aquatic environment has become a major reservoir for antibiotic - resistant microbes

    摘要臨床和畜禽業抗生素的濫用導致微生物在選擇性壓力作用下獲得並維持耐藥性，並有可能通過質粒和整合子將耐藥基因在相同或不同種屬中廣泛傳播轉移，最終導致多重耐藥。
  11. The transformation of hairy roots was confirmed by pcr amplification of rolb and rolc genes of ri plasmid from a. rhizogenes

    Pcr擴增結果證實， ri質粒的t - dna部分已在紅龍草毛狀根的基因組中整合及表達。
  12. It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s. tenebrarius

    Pcr實驗證明基因重組菌株中接合轉移質粒同源整合到黑暗鏈黴菌h6的染色體上。
  13. Dnd phenotype could be detected when the entire dnd cluster was integrated in the chromosome or carried by the low copy - number plasmid in zx1. however, when dnd cluster was carried by a plasmid with a copy - number around or higher than c. 10 copies in zx1, dnd phenotype could not be observed

    當完整的dnd基因簇整合在zx1染色體上或由1 2個拷貝的自主復制的質粒攜帶進入zx1 ，都能在zx1中檢測到dnd表型，但是由10個或超過10個拷貝的質粒攜帶進入zx1 ，就檢測不到dnd表型。
  14. In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k

    本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  15. Oer the course of seeral days, as the bacterium goes through its lifecycle, it transfers a portion of its plasmid out of its cell right into the mushroom cell, and integrates the introduced gene into the chromosome of the mushroom

    經過一些天之後，當細菌經歷了它的生命周期，它將從它細胞中質粒的一部分轉化入蘑菇的細胞中，然後把這個新的基因片段整合到蘑菇的染色體中。
  16. All of three orfs were demonstrated to be necessary for the dnd phenotype. for the survey of the biological relevence, different 5 "

    為了驗證這三個基因與dnd表型的關系，構建了一系列衍生於整合型載體pset152的質粒： phz2150 ， phz2151 ， phz2152和phz2153 。
  17. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  18. Integrated plasmids containing phage lambda promoter pr - directed and epitope - tagged 2. 7 kb pks gene were constructed for tagging the natural fr - 008 pks with specific immunodeterminant ( epitope ). these constructs were transferred into streptomyces sp

    構建了帶有噬菌體啟動子和特異性抗原決定簇（表位）及2 . 7kbpks基因的整合型質粒，用於標記天然的fr - 008pks 。
  19. Totally 99 transgenic rice plants from 125 resistant calli of 191 calli were obtained and pcr assay showed that 80. 3 % of them were positive. the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2. 5 copy of t - dna

    對抗性植株進行pcr擴增檢測，結果表明有80 . 3的抗性植株為陽性植株； southern雜交結果進一步證明了質粒的t - dna已經整合到水稻的基因組中，拷貝數為1 4個，平均每棵轉化植株有2 . 5個拷貝。
  20. It is widely used in genetic research and genetic engineering as a repository for fragment of dna incorporated into plasmids in its cytoplasm

    大腸桿菌細胞質中的dna片段可與質粒融合，並整合在細菌基因組中，所以被廣泛的用於基因研究和基因工程。
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