斑點序 的英文怎麼說
中文拼音 [bāndiǎnxù]
斑點序
英文
maculation-
Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization
用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing
豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。All the materials are traditional big groundnut which is proper in apearance, full in granule, mottle free, scar free, crack free and dilicious in taste
用榮成三鎮傳統大花生為原料，經嚴格挑選后加工，外型端正，顆粒飽滿，無斑點，無傷痕，無裂口，烘烤程序適中，食之香脆可口，略帶甜味。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。Now, in your favorite paint program, use a soft brush to make a popcorn shaped blob that looks something like this. make sure it ' s white on black background
現在，在你最喜歡的繪圖程序中，用一個軟點的筆刷來造一個斑點狀的爆米花，如下圖所示。注意，要用黑色的背景，白色的筆觸。In this paper, we analyzed the modulation of the interference intensity of each pixel on the time sequence speckle patterns in the time domain and proposed a new phase retrieve method, time sequence phase method ( tspm ), in which the time sequence speckle interferograms are used to obtain the whole field deformation of the object
本論文通過對序列散斑圖上各點在時間軸上散斑強度調制函數的變化進行分析，提出了一種基於時間序列的散斑干涉場的相位解調方法，進而獲得物體全場變形信息。Therefore, a - amylase has been used widely in many industrial fields, such as glucose production, beer brewing, fermentation trade, textile industry, and so on. the study on a - amylase is one of the most active fields in enzyme industrial fields. with the development of biotechnology, more and more scientists and researchers attempt to use dna shuffling technology to breed, screen and even " create " new a - amylase genes with higher activity and other new characters
設計引物時，上下游引物5 』端分別添加了kpn和bamh酶切位點，克隆得到的基因片段和質粒載體都用kpn和bamh進行雙酶切后，進行酶連、轉化、篩選得到陽性重組子，經過藍白斑驗證、單酶切驗證、雙酶切驗證、 pcr驗證等一系列的驗證后進行測序。In order to construct the recombinant plasmid pcdna3. 1 / ts87, first, the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i, and kozak sequence. then the rebuilt ts87 fragment was cloned into t - vector and transformed into e. coli dh5 a
通過重新設計引物在ts87兩端加上用於構建重組質粒的酶切位點hind和bamh和用於真核表達的kozak序列。將改造后的基因片段克隆入t - vector ，轉化大腸桿菌dh5 ，通過藍白斑篩選獲得陽性克隆后測序鑒定。The deduced amino acid based on cdna is very similar to channel catfish ( ictaluruspunctatus ), which is as high as. 83 %
推導的氨基酸序列與斑點叉尾刪腦型垮化酶的同源性最高（ 83 ） 。分享友人