The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap
基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap
基因導入
普通煙草中，經過
卡那黴素抗性篩選，最後獲得了轉pap
基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉
基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap
基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
The archaeological sites at sa caleta ( settlement ) and puig des molins ( necropolis ) testify to the important role played by the island in the mediterranean economy in protohistory, particularly during the phoenician - carthaginian period
薩?
卡萊塔聚居地考古遺址和
普伊格?德斯?墨林斯墓地遺址證實了一點：在史前，特別是腓尼
基-迦太
基時期，伊維薩島對于地中海經濟發展起到了非常重要的作用。
The roster of composers and conductors who have led the philharmonic includes such historic figures as theodore thomas, pyotr ilyich tchaikovsky, anton n dvo ? k, gustav mahler ( music director, 1909 ? 11 ), otto klemperer, richard strauss, willem mengelberg ( music director, 1922 ? 30 ), wilhelm furtw ? ngler, arturo toscanini ( music director, 1928 ? 36 ), igor stravinsky, aaron copland, bruno walter ( music advisor, 1947 ? 49 ), dimitri mitropoulos ( music director, 1949 ? 58 ), klaus tennstedt, george szell ( music advisor, 1969 ? 70 ), and erich leinsdorf
曾與樂團合作過的史上著名作曲家和指揮包括：西奧多?托馬斯、柴科夫斯
基、德沃夏克、馬勒、奧托?克倫佩勒、理查?施特勞斯、威廉?孟高貝格、富爾特文格勒、托斯
卡尼尼、斯特拉文斯
基、科
普蘭、布魯諾?瓦爾特、迪米特里?米特羅
普洛斯、克勞斯?騰斯泰德、喬治?塞爾、埃里克?雷恩斯朵夫。
At present, is included by the united nations the world culture and the natural heritage scenic spot historical site and the natural landscrape has 14, namely the london tower, ( the congress building ), the cloth roentgen heym palace ( nearby oxford ), the kanter uncle thunder host church, the buss city, salisbury suburb megalith and so on the vestige, the iron bridge canyon ( west of wool fertile hampton ), fang tingsi the monastery and the botanical garden ( york by north ), dallam ' s cathedral and the ancient castle, the harder good great wall ( area newcastle ), gwynedd ' s castle group ( area the welsh carnarvon ), scotland ' s saint kiel reaches the archipelago, north blue likes " road of the giant " being with pacific ocean ' s on england enjoys the german woods coral island
目前，被聯合國列入世界文化和自然遺產的名勝古跡和天然景觀就有14處，即倫敦塔、威斯敏斯特宮（國會大廈） 、布倫海姆宮（牛津附近） ，坎特伯雷主教堂、巴斯城、索爾茲伯里郊區的巨石陣等遺跡，鐵橋峽（伍爾沃漢
普頓以西） ，方廷斯修道院及園林（約克以北） 、達勒姆的大教堂和古城堡、哈德良長城（紐
卡斯爾一帶） ，圭內斯的城堡群（威爾士
卡那封一帶） ，蘇格蘭的聖
基爾達群島，北愛蘭的「巨人之路」和太平洋上的英國屬地享德森珊瑚島。
Application of combining urethane with procaine in experimental anaesthesia of rabbits
氨
基甲酸乙酯與
普魯
卡因在實驗兔麻醉中的聯合應用
The chapter on literature attempts to describe the essential features of numerous major writers ? joyce, proust, tolstoy, kafka, etc
本書關于文學的一章試圖描述眾多重要作家的
基本特點，如喬伊斯、
普魯斯特、托爾斯泰、
卡夫
卡等。
Based on an analysis of uhf ( ultra - high - frequency ) characteristic of pd ( partial discharge ) in power transformer, using mixing technique, a signal disposal cell has been devised for the sake of facilitating common data sampling card
摘要在分析了電力變壓器局部放電的超高頻特性的
基礎上，利用混頻技術，設計了局部放電超高頻信號的信號調理單元，方便
普通數字採集
卡採集。
9 colonel mahadou diop, the maharishi unified field based system of rehabilitation in senegalese prisons, annual seminar, dakar, senegal, feb. 1998. chris and janet attwood
9馬哈杜第爾
普：塞內西亞監獄以馬哈利西為
基礎更生課程，塞內西亞德
卡爾市年度研討會， 1988年2月。
By solving these problems, williams clarified the inevitability, the rationality, and the future development of mc. william ' s thought on mc is theoretically originated from the traditions of british empiricism and marxism. combining these two, he carried on a historical materialistic amelioration upon the empiricism, tried to explore possibility and approach of the general people to obtain enlightenments and to accomplish liberation from the reality, and presented a rather objective and reasonable evaluation on mc, which is totally different from the unsatisfaction and arrogance of lukas, adomo, horkheimer and marcuse to the reality and the mass
從理論來源上說，威廉斯的大眾文化思想是對英國經驗主義傳統和馬克思主義傳統的繼承，他在把兩個傳統相結合的
基礎上，對經驗主義進行了歷史唯物主義的改造，從個體經驗和實踐的角度探尋
普通大眾在現實生活中獲得啟蒙和實現解放的可能性和途徑，對大眾文化作出了較為客觀合理的評價，這與從盧
卡契到阿多諾、霍克海默到馬爾庫塞等人對現實和一般大眾表現出的不滿和傲慢態度是截然不同的。
Backed up by the soplat theory based on particle kinematics, the second chapter of this paper presents with analysis and simulation of several single observer passive measurement models, which uses such relative movement parameters as bearings changing rates and centrifugal acceleration information on the basis of bearings measurements. in the third chapter, the observability of location respectively using bearings and its changing rates information and centrifugal acceleration information is analyzed, and its observable condition is got. the fourth chapter puts forward the modified covariance extended kalman filtering ( mvekf ) against the defect of traditional extended kalman filtering ( ekf ), whose performance is simultaneously compared in the chapter with the performance of ususal tracking algorithm such as ekf, mgekf, iekf by computer simulation
在近年來提出的
基於質點運動學原理的單站無源定位理論
基礎上，本文第二章提出了幾種在角度測量的
基礎上增加角度變化率及相對運動的離心加速度等運動學參數的單站無源測量模型，並對它們進行了分析和模擬；第三章分別對利用角度及其變化率信息定位和利用離心加速度信息定位的可觀測性進行分析並得到了相應的可觀測條件；第四章針對傳統擴展
卡爾曼（ ekf ）方法的缺點，提出了一種修正協方差的擴展
卡爾曼濾波（ mvefk ）方法，並將其和ekf 、 mgekf 、 iekf等常用的單站無源定位濾波方法進行了性能模擬比較；第五章通過引入雷達機動目標跟蹤方法和模型，提出了利用角度及其變化率對機動輻射源跟蹤的多級噪聲自適應方法和imm方法；第六章主要對角度變化率和離心加速度參數的獲取技術進行了研究，提出了幾種高精度測量脈沖序列多
普勒頻率變化率的方法。
Marburger set about defraying tensions between the laboratory and local residents. “ he built a culture that involved the community, and that just hadn ' t happened at that facility before, ” remembers scott cullen of the standing for truth about radiation ( star ) foundation, an easthampton, n. y., advocacy group that had fought the laboratory
紐約東漢
普頓的尋找輻射真相
基金會，曾是對抗該實驗室的團體，團員
卡倫回憶說：他建立了讓社區也共同參與的一種文化，那是該機構前所未有的做法。
Several carrier frequency estimation algorithms including ekf and rls with opened loop are analyzed, which aim to settle the carrier phase estimation problem preferably under high dynamic circumstances. an improved phase and frequency esti - mation algorithm is proposed in the paper. simulation results show that the algorithm is faster and more precision than other algorithms
為更好地解決高動態環境中的載波同步問題，本文進一步研究了存在動態多
普勒頻移情況下的開環相干載波同步問題，包括
基於擴展
卡爾曼濾波、最小二乘演算法等的頻差、相差聯合估計方法。
From the whole perspective, the research into of economics methodology is currently transforming from the positivism to historism and intergralism. in 1980, lakatos ’ s refinement falsificationism starts up the upsurge of hot debate and research since it is based on the combination of popper ’ s thought of with kuhn ’ s insights into historical methodology. his theory is adapted more to be applied in economics methodology than that of popper ’ s
20世紀80年代，科學哲學方法論中的拉
卡托斯的精緻證偽主義掀起了經濟學界關于經濟學方法論的大討論和研究熱潮，因為拉
卡托斯的思想是在融合了波
普爾的樸素證偽主義與庫恩的歷史主義的方法論
基礎上形成的，他的理論比波
普爾樸素證偽主義更適合在經濟學方法論中應用。
Los angeles ap jessica simpson and nick lachey who turned mid - level music careers into a hollywood star - spangled marriage have separated following months of persistent breakup rumors
洛杉磯在持續幾個月的分手謠言后，傑希
卡。辛
普森和尼克。拉
基正式分居。
Jessica simpson has reportedly hired a high - powered divorce attorney to handle her split with husband nick lachey just three weeks after they announced their separation. jessica simpson has reportedly hired a high - powered divorce attorney to handle her split with husband nick lachey just three weeks after they announced their separation
日前，有消息稱傑西
卡辛
普森已雇傭了詹妮弗安妮斯頓離婚時的辯護律師來解決她與丈夫尼克拉
基的離婚問題，因為她要向安妮斯頓那樣「優雅」地離婚。
The card is based on the flat roof of pc machine, at first, we are used of the source of program data to leave in ide care, then binary file is come by the send out card, which is transmitted the standard ' s transmission stream. according to the european the standard of dvb - c, variable transmission current velocity rate is 9. 6 - 38. 4 mbits / s
此
卡基於pc機平臺，首先將節目源數據以二進制文件的格式存放在
普通ide硬盤上，然後通過計算機的pci總線將二進制文件經過發送
卡轉換成符合數字電視asi （異步串列介面）介面標準的平穩碼流。根據歐洲dvb - c標準，可變的傳送流速率為9 . 6 ~ 38 . 4mbit s 。
Ng was a jefferson fellow of the east west center in hawaii where he studied political and economic developments of the pacific rim countries ( 1985 ). he was also trained in bbc, london ( 1974 ) and in 1976 he was awarded a scholarship to study financial journalism at the thomson foundation in cardiff
吳先生於一九八五年在夏威夷東西文化中心修讀太平洋沿岸地區的政治和經濟發展，在一九七四年遠赴倫敦的英國廣播公司受訓，並於一九七六年獲頒發獎學金，前往威爾斯
卡迪夫的湯
普遜
基金會修讀財經新聞學。
When i came to this post three years ago, i had conflicting reports concerning china s population practices. some said that there was no coercion that would trigger the kemp - kasten prohibition of u. s. funding to the un population fund, unfpa. others said that there was. so the state department dispatched a blue ribbon team in may 2002 to get the facts
杜威指出： 「聯合國人口
基金對中國計劃生育活動的支持和參與使中國政府能加緊推行強制墮胎的政策，從而觸及了肯
普-
卡斯滕修正案中禁止向任何支持或參與實施強迫墮胎或強制絕育項目的組織提供幫助的條款。 」
The team will be gathering information to assist the administration in determining whether or not the united nations population fund s china program is in violation of u. s. law ( the kemp - kasten amendment ) and whether the fund is therefore eligible to receive u. s. government funding as appropriated
該小組將收集有關信息，以幫助布希政府確定聯合國人口
基金的中國項目是否違反美國有關法律（即《肯
普-
卡斯滕修正案》 ） 、進而確定該
基金是否能夠獲得美國政府的撥款資金。
The port director, matagi ray mcmoore, said the new zealanders and many of the other 100 people stuck on the island had requested assistance to be ferried to samoa so that they can catch flights home
管理部負責人馬塔基雷麥克摩爾透露包括停留在普卡普卡島上的一些紐西蘭人在內的總共100多人向他們請求支援，幫助他們及時乘船到達薩摩亞以便能及時趕上回家的班機。
