普通菌素 的英文怎麼說
中文拼音 [pǔtōngjūnsù]
普通菌素
英文
vulgarin-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted
因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明， arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝： （ 1 ） arg可能影響胞外蛋白酶的活性，進而促進含氮大分子物質的分解代謝，補充發酵過程中的氮素來源。In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated
將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。Ann arbor, mich. ? antibacterial soaps show no health benefits over plain soaps and, in fact, may render some common antibiotics less effective, says a university of michigan public health professor
密西根州，安拉伯據密西根大學一公共衛生學教授透露，抗菌藥皂非但不比普通香皂更有利於身體健康，還有可能使某些常用的抗生素療效降低。Streptomyces tenebrarius a04, the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper. after treatment of spore suspension and mycelia shivered by supersonic with mutagen, combined with the application of screening models, some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23, a2 - 30, asm6 and al - 16 were obtained
本文以尼拉黴素單一組分?安普黴素產生菌s . tenebrariusa04 （發酵單位為2847u ml ）為出發菌株，通過對單孢子懸液和超聲波破碎菌體的誘變處理並復合篩選模型，獲得了遺傳特性穩定的單組分高產菌株： a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ，搖瓶發酵單位達4800 - 5200u ml 。It is not affected by any antibiotics or common bacteriastatic agents.
它不受任何抗菌素或普通制菌劑的影響。To study the biosynthetics genes of apramycin in streptomyces tenebrarius, the apramycin resistant gene was isolated by gunshot cloning firstly
為了研究黑暗鏈黴菌安普黴素生物合成基因，首先通過鳥槍克隆的方法克隆其安普黴素抗性基因。The results from analysis of the ecological diversity in the field soils and polluted soil showed that the frequency of occurrence of the genus glomus was highest and acaulospora was the second most abundant genus
分析不同土壤條件下am真菌的多樣性特點及其影響因素發現，重金屬污染土壤和普通農田土壤中均以glomus屬的頻度最高。分享友人