最低培養基 的英文怎麼說
中文拼音 [zuìdīpéiyǎngjī]
最低培養基
英文
minimal medium-
Rat cumulus - enclosed oocyte ( ceo ) and denuded oocyte ( do ) both spontaneously resume meiotic maturation in vitro, when cultured for 2h, the germinal vesical breakdown percentage of do reaches up to 88 %, and ceos get 53 %. oocytes begin to extrude first polar body 1 ( fb1 ) when cultured for 8h. mouse oocyte spontaneous maturation in vitro can be inhibited by 4mm hypoxanthine ( hx ) or 25um 3 - isobutyl - methylxanthine ( ibmx ). while, 4mm hx can not inhibit the spontaneous maturation of rat oocyte, and the lowest effective concentration of ibmx to inhibit rat oocyle spontaneous maturation in vitro is 50um. treated with the same concentration of ibmx, gvbd percentage of rat ceo is lower than that of do, the results indicat that cumulus cells delay rat oocyte spontaneous maturation and potentate the inhibition effect of ibmx, but it ' s not the case on mouse
Ceo和do培養8h時都開始排出pb1 ；在卵母細胞成熟抑制模型中， 4mm次黃嘌呤（ hx ）和25 m的異丁基甲基黃嘌呤（ ibmx ）是小鼠卵母細胞成熟的有效抑制劑，但4mm的hx抑制大鼠卵母細胞成熟效果不理想，用hx - m199和hx - mem培養24h ， gvbd率分別為61 ， 81 ，而ibmx有效抑制大鼠卵母細胞自發成熟的最小濃度為50 m ， ibmx可以降低大鼠ceo和do的gvbd率，但ceo中的卵母細胞gvbd率要顯著低於do的gvbd率， 100 m的jbmx處理24h后，大鼠ceo和do的gvbd率分別為24 ， 84 ，提示卵丘細胞的存在可以延遲大鼠卵母細胞的自發成熟，有助於抑制卵母細胞成熟，但在小鼠上ceo和do的自發成熟差異不明顯。The manufacture of thiamine from the simple substances present in the minimal medium does not take place in a single chemical reaction.
從最低培養基中的簡單物質製造硫胺素，不是通過單一的化學反應。Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased
腦內gfap陽性結構也明顯增多，其分佈與fos陽性細胞分佈基本一致，表現為胞體肥大、突起粗長； ast緊密包繞在神經元周圍形成神經元- ast復合體（ n - asc ） ；在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ； （ 2 ）免疫電鏡下son內星型膠質細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區（ edas ） ，高滲刺激后數量明顯增多： （ 3 ）將們個mp注入大鼠一側n卜、卜卜或孤束核（ ws ） ，分別在延髓內臟帶（ mvz ） 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ； （ 4 ）高滲刺激使培養神經元和ast內鈣水平先升高后降低，最後維持在比高滲刺激前稍高的靜息鈣水平上。Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0The results showed that high concentration of 2, 4 - d was required for callus induction from mature seeds of tall fescue, and combination of 8mg / l 2, 4 - d with 2mg / l aba gave best induction effects. by slicing sterilized seeds longitudinally or cutting embryos, callus induction frequency was profoundly increased over intact seeds from one and half to eight times. adoption of ms basal medium and supplementation of 0. 5g / l casamino acids and 0. 5g / l glutamine in medium were found to help to facilitate callus induction
研究表明，高羊茅成熟種子愈傷組織誘導需要較高濃度的2 ， 4 - d ，以8mg l2 ， 4 - d與2mg laba配合能獲得最佳的誘導效果；種子滅菌后縱切或切胚，可使出愈率成倍提高；採用ms基本培養基和在培養基中添加0 . 5g l的水解酪蛋白與谷氨酰胺也有助於提高出愈率；低劑量（ 10gy ）射線輻照處理對成熟種子愈傷組織尤其是胚性愈傷組織形成有一定的刺激效應。By using inverted microscope, it was observed that dunaliella salina of different growth stages after the high osmotic shocks can live in the medium with nacl concentration between 0. 1m and 5. 0m, but its growth status and period showed differently. the optimal concentration for the growth of dunaliella salina was 0. 5 - 1. 5m, and this organism could stand a variety range of osmotic shock. enolase gene, the anti - adversity gene of d. salina, was cloned by modified degenerate pcr technique
通過倒置顯微鏡觀察生長在不同鹽濃度，不同生長時期，以及經不同滲透壓震動的鹽藻，四川大學博士學位論文發現其在o . im一5 . omnaci培養基中均能正常生長，但其生活狀態及生長周期有所不同，其最適生長naci濃度為0 . 5一1 . 5m ，還能適應各種高滲及低滲震動。At last, we have some advises about how to bellowing human capital gini coefficient : region a should let the high rank human capital to flow out, cultivate the middle rank human capital, support and ensure the low rank human capital and make the structure of college education more rational. region b should reduce human capital gini coefficient basing on improving the level of people been educated. region c should strengthen the support of nine years duty education, develop the career technique education and whole life education, reduce the human capital being
最後，本文對不同區域分別提出了減小其人力資本基尼系數的建議:區域a應該積極引導高層次人力資本的向外流動，繼續堅實中等人力資本的培養，對低層次人力資本應給予有力的支持與保障，同時注意高校安排教育結構的合理性;區域b應該在普遍提高全民受教育水平的基礎上逐步縮小人力資本基尼系數;區域c應加強政府對普九工作的支持力度，注意發展職業技術教育和終生教育的教育系統以及減緩該區域的人才流失現象。And then, thesis explicates the reasons of the difference from five aspects including natural resource, area conditions, labor conditions, technical conditions and industry structure. furthermore, the thesis points out that the sustainable utilization of natural resource, the adjustment of labor structure, improvement of labor qualities, the employment of advanced science and technology and reasonable industry structure are critical to the improvement of rural economy ' s integral, power and comprehensive competition. at last, this thesis puts forwards a series of policies and countermeasures, such as accelerating urbanization of villages of villages to develop increasing pole, enchanting agricultural, industrialization to strengthen the industry foundation, improving the help and development policies to the poor to strengthen the district economic cooperation and the coordination effect by decreasing the limits of the poor, and so on, which can encourage each area display its advantage and supply what the other needs and make the rural economy coordinated and commonly flourishing
根據分析結果將11個市劃分為省內發達區域、中等發達區域和不發達區域三種類型，將138個縣（市）劃分為由高到低七個等級；對差異產生的原因從自然資源、區位條件、勞動力條件、技術條件、產業結構等五個方面進行了系統探討；並從更深層次上指出，土地等自然資源的可持續利用，勞動力構成優化、素質提高，先進科學技術的適用，產業結構的合理布局等對農村經濟的整體實力和綜合競爭力的提高起決定性的作用；最後，在河北省農村經濟協調發展的角度，提出了「因地制宜，揚長避短；優勢互補，共同發展」的指導思想和「鼓勵先進，鞭策后勁；區域聯動，全面協調」的基本原則，並進一步提出了加大小城建設? ?培養增長極，推進農業產業化經營? ?強化產業基礎，改善扶貧開發政策? ?降低后勁制約以及加強區域經濟聯合? ?增強協調效應等綜合措施。Part two : organizing strategy of cooperative learning in implementation of new mathematical curriculum this part analyzes the problem in the process of implementing of cooperative learning in new mathematical curriculum by observing in classroom and studying case. six problems is : ( 1 ) the group activity stay on the formal, and lack the material cooperation ( 2 ) individual duty diffuse, " take a ride " phenomenon have the occurrence ; ( 3 ) student social intercourse technical lack. ( 4 ) no change in teacher ' s role, lacking guidance to student ; ? ividing group difficultly for big class formation, fulling of too much noisy in classroom ; ? valuation system is not well, group cooperation exist in name only, provides six suggestions for these problems : ( 1 ) dividing group scientificly and vividly ; ( 2 ) leading student active cooperative learning ; ( 3 ) increasing the individual duty ; ( 4 ) cultivating students " cooperative method and social intercours technical ; ( 5 ) enhancing management in classroom ; ( 6 ) handling a few relations rightly ; finally, puts forward the principle and program of orgnizing cooperative learning in new mathematical curriculum
二、新數學課程實施中合作學習的組織策略本部分在對實驗區數學課堂觀察和個案研究的基礎上，分析得出了新數學課程實施中合作學習存在的6個問題：小組活動留于形式，缺乏實質性合作；個體責任擴散， 「搭車」現象時有發生；學生社交技能欠缺，合作效率低下；教師對自己角色轉變認識不清，指導作用沒有跟上；大班額分組困難，課堂過于喧嘩混亂；評價體系沒有跟上，小組合作名存實亡。針對這些問題，對新數學課程實施中開展合作學習提出了6方面建議：科學靈活地分組；引導學生主動合作學習；提高個體責任：重視對學生合作方法、社交技能的培養；加強合作學習中課堂管理；正確處理幾對關系。最後提出了新數學課程實施中合作學習的組織的原則和組織序列。The author, after years " practical teaching and research, draws a conclusion that : successful contemporary prose teaching can foster the students " faithful attitude to life, and can teach students how to use the language basically and proficiently, can also enrich students " humanism and it plays an important role in forming students " better individuality and perfect personality
筆者通過教學實踐和研究后認為：成功的現代散文教學既培養學生真誠面對人生的態度，又教會學生熟悉運用最基本的語言。在豐富學生的人文內涵，促使學生形成良好個性和健全人格方面起著不可低估的作用。High sucrose and agar is apt to keep the tight close structure of callus. ( 4 ) the optional inducing medium of adventitious buds is ms basal medium with 2mg / l 6 - ba, 30g / l sucrose and 9g / l agar. all the tested genotypes have high inducing rate on this medium
供試的4個基因型在此培養基上不定芽誘導效率均最高，由高到低依次為：松93 - 8為962 . 9 ；東農v10為679 . 7 ；東農419為542 . 5 ；富士光為482 . 6 ，與其它處理差異達到顯著。According to morphologic characteristics. when cultured in liquid medium, cadmium with initial concentration below 4. 61mg / l did n ' t inhibited the growth of f2, 163. 8mg / l of cadmium affected it obviously but growth continued to a great extent. cadmium removal from medium by f2 under liquid culture was not so stable with low cadmium concentration below 4. 61mg / l, but removal ratio reached 96 % with initial cadmium concentration of 163. 8mg / l and cadmium content of biomass reached 28 %, which showed high capacity of cadmium accumulation by f2
F2在液體培養時，培養基初始鎘濃度在4 . 61mg l以下時對其生長無抑制作用，鎘濃度為163 . 8mg l時有較大影響，但仍有明顯生長； f2對液體培養基中低濃度鎘（ 4 . 61mg l以下）的去除效果不太穩定，但初始鎘濃度為163 . 8mg l時，其去除率為96 ，菌體最大鎘含量可達28 ，顯示了較大的富集容量。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。No callus occurred from hairy roots on medium lacking 2, 4 - d. high concentration ( l. 0mg / 1 ) of 2, 4 - d could induce much more calli, but it obstructed the growth of calli and followed buds differentiation. the result of callus induction showed that the optimum medium for callus induction was ms + 0. 5mg / l 2, 4 - d + 0. 5mg / l 6 - ba and ms + 0
愈傷組織誘導最佳培養基為： ms 05mg l個d 05mg l ba ，愈傷組織生長的最佳培養基為ms 0ling 12 ， 4 d cling 16ea ；愈傷組織能在不含激素的培養基上自發地分化出不定芽，但分化頻率較低，而6ea對不定芽的分化作用較明顯，配合使用6習a和naa對芽的誘導及得芽率較好，不定芽誘導的最佳培養基為ms 10mgilnaa 05mg lia培養基。分享友人