有毒菌株 的英文怎麼說

中文拼音 [yǒudújūnzhū]
有毒菌株 英文
virulent strain
  • 有 : 有副詞[書面語] （表示整數之外再加零數）: 30 有 5 thirty-five; 10 有 5年 fifteen years
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 株 : Ⅰ名詞1. （露在地面上樹木的根和莖） root and stem of a tree above the ground 2. （植株） individual plant; plant Ⅱ量詞（棵）
  • 有毒 : poisonous; venomous; deleterious
  • 菌株 : bacterial strain
  1. The virulent strains had a polysaccharide capsule and formed smooth colonies on agar medium.

    有毒菌株具有多糖莢膜它在瓊脂培養基上形成光滑的菌落。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  5. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  6. If etec vaccine contains colonization factor antigen such as cfa / i and cs1 - cs6, associating with enterotoxin ( lt ), it can, theoretically, protect the user from diarrhea by percent 80 etec attacking

    如果一個疫苗包含有定居因子抗原和lt腸毒素，則理論上可使服食者免受80的etec菌株的侵害。
  7. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  8. Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them. the homological cry3aa gene was expressed in escherichia coli. and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page

    生物活性測定結果表明該菌株對榆藍葉甲（ pyrrhaltaaenescens （ fairmaire ） ）和光肩星天牛等鞘翅目昆蟲具有較高的毒力，因此初步確認該菌株屬于cry3類； （ 2 ）發現該菌株中編碼毒蛋白的基因位於質粒上，並且已經成功地克隆到該基因。
  9. Summarizing the effects of pbmblc on the three strains, it is known that genetically engineered strain construction using this method can not get sati sfactory results, though the potency of ybt - 1535 ' s transformants was higher than ybt - 1535. because of the low toxicity level of ybt - 1535, the transformants with higher potency cannot be utilized commercially

    綜合這三個菌株的實驗結果，雖然菌株ybt - 1535轉化子的綜合毒力有所提高，但那是建立在ybt - 1535較低毒力水平之上的，轉化子的毒效離實際應用相差甚遠，工程菌構建的基本思路可能需作大的改變。
  10. This thesis studied on bacillus thuringiensis strain bt886 which was separated and selected by researchers of our laboratory. according to the observation of crystal shape and the bioassay of motschulsky and fairmaire, bacillus thuringiensis strain bt886 was identified as cry3 type, and the conclusion was assured by the further study on molecular level. the 1956 base pairs full lengrh homological cry3aa gene which was toxic to motschulsky was cloned and sequenced

    以該菌株為材料，克隆出了對光肩星天牛（ anoplophralabripennis （ motsch . ） ）具有毒殺作用的cry3aa同源基因，並且對該基因進行了人工改造、人工合成、大腸桿菌表達、生物活性測定、雙元表達載體的構建以及對楊樹的轉化等一系列研究，主要結果如下： （ 1 ）顯微觀察該菌株所形成的伴孢晶體為方形。
  11. But does not harm other organisms. the toxin gene in different strains of b. thuringiensis can affect different mixes of insects so seed makers can select the version that seems best suited to a particular crop

    不同的蘇力菌菌株，各有不同的毒基因，影響的昆蟲也不同，所以種籽生產商可以針對特定的作物，選用最適合的抗蟲基因。
  12. The chromosome walking adapter consisting of 40nt and 44nt oligonucleotides was designed and synthesized

    結果顯示， b8和b8f具有弱的一過性毒力，而b8b菌株無毒力。
  13. New minimally heated refrigerated foods are raising concerns about the hazard, he will tell delegates. other topics include how the complete genetic blueprints or genomes of food - poisoning bacteria such as campylobacter are transforming the science of food safety, and the latest research into potentially deadly strains of the e. coli gut bacterium

    本次研討會的其它議題還包括：諸如彎曲桿菌這類食源性有毒細菌的完整基因圖譜或基因組圖譜是如何轉化成為食品安全科技的，以及對大腸桿菌這種腸內細菌有可能轉變成致命菌株的最新研究成果。
  14. London, march 5 ( reuters ) - an experimental bird flu vaccine for humans provides substantial cross - immunity against " drifted " strains of the virus, suggesting it may provide protection in the event of a pandemic, its maker said on monday

    倫敦（路透社） 3月5日-一種人用禽流感疫苗經過實驗證實具有免疫能力能對抗「漂浮」病毒菌株，它的發明者星期一發表報道說此疫苗可以提供保護以預防禽流感的流行。
  15. Britain ' s glaxosmithkline plc ( gsk. l : quote, profile, research ) said clinical data from two studies showed its pre - pandemic shot was able to recognise and kill an indonesia h5n1 strain that was genetically quite different from the vietnam one included in the vaccine

    倫敦（路透社） 3月5日-一種人用禽流感疫苗經過實驗證實具有免疫能力能對抗「漂浮」病毒菌株，它的發明者星期一發表報道說此疫苗可以提供保護以預防禽流感的流行。
  16. The transformants of strain ybt - 1535 displayed significantly higher toxicity against s. exigua than strain ybt - 1535 ( at least 8 times ), but the toxicity against heliothis armigera and plutella xylostella changed little. for the strain ybt - 1520, the effect was not the same as strain ybt - 1535

    結果菌株ybt - 1535的轉化子對甜菜夜蛾的毒力有非常顯著的提高（ 8倍以上） ，對棉鈴蟲和小菜蛾的毒力影響不大，轉化子的綜合毒力高於ybt - 1535 。
  17. Based on genetic diversity, natural strains of a. implicatum, that contained plant fitness - enhanced characters but toxin - free to livestock, would be able to selected for brachiaria breeding. based on rapd analysis, a common product about 500 - bp to all isolates of a. implicatum that amplified with primer opak10 was cloned and named as p ai ak10 - 500

    Implicatum的自然株系中篩選有利旗草生長、但卻不產生家畜毒性物質的優良菌株提供了可能，為旗草的抗性育種和高產育種提供了新的資源和方法。
  18. Current drug therapies are not practical for global control due to the high cost, problems with patients " compliance and the emergence antibiotic resistant strains. therefore, vaccination against h. pylori has been considered to control h. pylori infection

    藥物治療hp存在較大的局限性（藥物毒副作用、費用昂貴、患者依從性差及耐藥菌株不斷增多等） ，而疫苗接種可使人群獲得持久、有效的免疫力，是從人群中消除hp的有效措施之一。
  19. During antiretroiral therapy that does not fully suppress the irus, a strain that deelops drug resistance will grow more quickly than strains lacking such resistance, and the resistant strain will replicate to become the most prominent irus in the person ' s body

    在沒有完全抑制病毒的抗逆轉錄病毒的治療中，發展成耐藥菌株將比其他缺少這樣抗藥性的菌株生長更快。
  20. The fact that influenza viruses change from year to year implies that the immunity built up from having the influenza caused by one virus strain does not always provide protection when a new strain is circulating. besides, immunity to the disease declines over time and may be too low to provide protection after one year

    因為流感病毒的類型每年都會轉變，患上某種病毒菌株所致流感而產生的免疫力，未必足以抵禦當時流行的新菌株而且，流感免疫力會隨時間降低，一年後可能降至沒有保護作用的水平。
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