有絲分裂期 的英文怎麼說
中文拼音 [yǒusīfēnlièqī]
有絲分裂期
英文
m phase-
During mitosis, sister chromatids remain joined by their centromere until anaphase
在有絲分裂期間,姐妹染色單體在分裂後期之前都是通過著絲點連接的。Anaphase the stage in mitosis or meiosis when chromatids are pulled toward opposite poles of the nuclear spindle
後期:在有絲分裂或者減數分裂過程中染色單體分別朝相反的兩極運動。Chromomere a small beadlike deeply staining structure seen in chromosomes during prophase of mitosis and meiosis
染色粒:在有絲分裂及減數分裂的前期存在於染色體上的小念珠狀的著色深的結構。140min, chromosomes of the daughter cells are situated at metaphase of the second karyokinesis, while the daughter cells do n ' t separate
120min ,染色體處于有絲分裂末期,分裂溝形成,受精卵徑裂為二細胞。受精140min ,再徑裂為4細胞。( 3 ) the chromosomes obtained by air - drying and chromosomes from the silkworm genital gland are similar in shape. but the numbers and the length of the chromosomes are obviously different. the chromosomes obtained from the genital gland have little difference in length, while the chromosomes of bmn cell are different in length very much
但是,在染色體的數目上有顯著的差異。另外,染色體間的長短差別也非常明顯。由家蠶生殖腺得到的減數分裂中期染色體和胚胎得到有絲分裂的中期染色體,其染色體長短差異不明顯,而binn細胞的中期染色體長短非常不一致,差異非常顯著。Study on function of mapk and mpf in mitosis of mouse fertilized eggs
對小鼠受精卵有絲分裂期作用At the onset of mitosis, prophase, the nucleolus breaks down.
有絲分裂的開始稱為前期,核仁消失。Cell plate a structure that appears in late anaphase in dividing plant cells and is involved in formation of a new cell wall at the telophase stage of mitosis
細胞板:植物細胞分裂晚後期出現的一種結構,它和有絲分裂末期新細胞壁的形成有關。Sds - page and western blotting of plasmodia of physarum polycephalum showed that cyclin b was present in the plasmodia of s phase, g2 phase, prophase, and metaphase. the molecular weight of cyclin b in physarum polycephalum was around 62kda. cyclin b was gradually accumulated from s phase to metaphase, and reached the maximal level at metaphase, however disappeared in the plasmodia of anaphase and telophase
Westernblotting分析結果表明, cyclinb存在於s期、 g _ 2期、前期和中期的原質團中,分子量約為62kda , cyclinb的含量從s期到中期逐漸增加,特別是有絲分裂前期增加明顯,中期原質團cyclinb含量最高,后末期原質團中沒有cyclinb 。The reasults are summed up as following : ( 1 ) the more metaphase chromosomes of bmn cells and moderately contraction can be obtained by air - drying after cultured at the colchicine concentration 1. 0 u g / ml, 28 " c, for 6 - 9h
用空氣乾燥法和培養細胞貼片法對bmn細胞的染色體和有絲分裂進行研究發現: ( 1 )採用空氣乾燥法制備bmn細胞的染色體,在終濃度0 . 1ug / ml的秋水仙素28培養6 9h條件下可得到較多中期分裂相,染色體的凝集也比較適中。Cytoskeleton of a cultured epithelial cell. microtubules are shown in green and dna is shown in blue
處于有絲分裂間期的動物上皮細胞。綠色為微絲,紅色為肌動蛋白,藍色為染色質,中心點復製成兩個。The results demonstrated that the activities of pod isoenzymes increased under the sound. compared with control group, there was no new enzyme band in pod electrophoretogram for stimulation groups
結果表明刺激組與未經聲波處理的對照組相比, g0 / g1期細胞減少, s期細胞增多,說明聲波刺激促進了細胞的生長,有助於細胞有絲分裂。W7 was added when the cells entered anaphase, we found that although some cells had elongated, the distribution patternof cam at central spindle was normal, and it seemed that a cleavage furrow would possibly form soon, however, these cells stopped cytokinesis at this stage, no furrow ingression followed
在細胞進入有絲分裂後期時,加入w7 ,我們發現有的細胞雖已拉伸變長,中區紡錘體處也有gfp cam的分佈,存在形成分裂溝的跡象,但分裂溝卻不繼續縊裂加深。On the other hand, the activity and function of p27 kip1 was affected by many physiological agents such as camp, il - 2, rampamycin. the expression of p27kip1 increased after - irradiationin in the fibroblastoma cells, leading to arrest in gl phase, but its expression markedly decreased after y - irradiation in the nbcc cells, and leaded to arrest in g2 / m phase. several studies have reported that p27 kip1 involved in a much wider range of cellar processes, such as differentiation, apoptosis and migration, the expression level of p27kip1 is key for its function in dna damage responses
另一方面, p27kip 』的活性水平及功能作用,因細胞類型不同而各異,又受多種因素的影響,如一些有絲分裂原camp 、雷帕黴素可以上調p27kip ,的表達,而il一2等則與p27ki 」 『的表達下降有關,受電離輻射( ir )照射的成纖維細胞瘤及用1 , 25一二經基d3處理的hela細胞系在gl期均有游離的p27腳『的積累,細胞周期阻滯在gl期;而在痣癌伽bccs )細胞中, y一射線能誘導p27吻『表達水平的顯著下調,細胞周期阻滯在g2艦期。Their flowers and fruits were removed in this experiment to enhance them to take branch roots on the stems, and then the branch roots were used as materials. five mitosis metaphase cells, with their chromosomes well scattered, were counted. chromosome number of hup is 2x = 24, and its karyotype formula is 2n = 2x = 24 = 8m + 12sm + 4st, being to " 3b " of stebbins
本實驗採取去其花、果實,促其莖節上長側根的辦法,以其側根為實驗材料,統計了5個分散較好的有絲分裂中期細胞,其染色體數目為2x = 24 ,核型公式為2n = 2x = 24 = 8m + 12sm + 4st ,核型類型為3b型。At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage
在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化,結果表明,分裂期細胞總體熒光強度強于間期細胞;而對同一細胞內熒光強度的分析說明,間期細胞的熒光主要分佈於胞質中,細胞核內則分佈較少;而正在進行有絲分裂的細胞內熒光主要集中於赤道板處;剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1
本實驗以多頭絨泡菌原質團為材料,採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法,研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。Rt - pcr kit was from takara technology co. ltd. the sequence of the primer of pkb are shown below, together with all the sodn and saodn oligodeoxynucleotide were synthesized in takara technology co. ltd. pkb primers ; forward primer 5 ' cga gaa gcc gcg acc caa cac3 ' and reverse primer 5atg cac tac cgc cgg a3 "
有絲分裂前, cdcz / cy山b 、組蛋白h ;激酶的活性通過被weel和myt激酶磷酸化cdcz的1卜巧和thr14殘基而抑制。在有絲分裂期mpf可以被cdc25活化,這兩個位點被去磷酸化, cdc25是蛋白水解酶,可以使cdcz的t打巧脫離磷酸化。Polyclonal anti - cdc2 antibodies, anti - cdc25c monoclonal antibodies and peroxidase - conjugated horse anti goat igg were purchased from sigma chemical co. ( stlouis, mo ) reagents [ a - 32p ] dctp and [ r - 32p ] atp were purchased from yahui biotech - nology co. ltd, china
在真核細胞中,進入分裂其是依賴于mpf ( mphasepromotingfaetor ) , mpf由edez激酶和eyelinb組成,通過磷酸化在有絲分裂期起作用的底物而發揮作用。B - actin primers : forward 5tcc tat cgg cct cct cct ac 3 " and reverse 5aga atg tag tcc gcc agg tc 3 ", designed for 200bps. saodn oligodeoxynucleotide of pkb : stac agc ctg aga gga cct acc tg3 "
在有絲分裂期, edc25經歷216位ser磷酸化同時其水解酶活性增強,活化的mpf磷酸化並活化edc25 ,形成正反饋,結果進一步活化mpf前體,活化的mpf促進細胞周期g2 / m期轉換。分享友人