木聚糖酶 的英文怎麼說
中文拼音 [mùjùtáng]
木聚糖酶
英文
xylanase-
Studies on the xylanase producer aspergillus niger
黑麴黴產木聚糖酶的研究Xiaoxia wang, baishan fang, juxiang luo, wenjun li, liaoyuan zhang. heterologous expression, purification, and characterization of xylose reductase from candida shehatae
王曉霞，鄭晨娜，王飛飛，方柏山.共表達木聚糖酶和木糖還原酶生產木糖醇的研究Xylanases can hydrolyze xylans into xylooligosaccharides and d - xylose
此菌株能胞外分泌木聚糖酶。The metabolism of these extreme microbes during the production of maotai liquor would further produce multiple enzymes of thermal stability such as amylase, protease, saccharifying enzyme, cellulose, glucase, xylanase, and each kind of dehydrase involved in redox reaction, and dna polyase etc
茅臺酒釀造過程中極端釀酒微生物代謝產生多種熱穩定性的酶，如澱粉酶、蛋白酶、糖化酶、纖維素酶、葡萄糖甘酶、木聚糖酶、參與氧化還原反應的各種脫氮酶、磷酸烯醇丙酮酸激酶及dna聚合酶等。Study on the production of xylanase from streptomyces microflavus
細黃鏈黴菌產木聚糖酶的研究Application research on kraft wood pulp bleaching with xylanase pretreatment
木聚糖酶在硫酸鹽木漿輔助漂白中的應用研究The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase
尤其是在木聚糖酶譜帶檢測中發現，突變株發酵液中有三種類型的木聚糖酶，而出發菌株中只有兩種類型的木聚糖酶，並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現，突變株中第三種類型的木聚糖酶不僅表達量很大，活力也很高。The xylanase activity of the mutant kept stable after 10 generations. after orthogonal designing experiment, the optimum fermentation conditions of a. niger j 506 were obtained, which is as followed : concentration of the major carbon resource 4 %, ratio between bran and corncob 5 : 5, concentration of glucose 0. 1 %, concentration of ammonium oxalate as supplemental nitrogen resource 2. 0 %, the initial ph of liquid medium 5. 0, 100ml / 250ml flask
經過正交試驗設計，得出突變株a . nigerj506產木聚糖酶最佳的工藝條件為：主碳源濃度4 、麩皮與玉米芯的比例為5 ： 5 、輔加碳源葡萄糖的濃度是0 . 1 、輔加氮源草酸銨的濃度是2 . 0 ，培養基初始ph為5 . 0 ， 250ml三角瓶的裝液量為100ml 。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。Isolation and identification of endoxylanase from bacillus subtilis
枯草芽孢桿菌內切木聚糖酶的分離純化與鑒定Study on h - bleaching of pretreatment with xylanase
木聚糖酶預處理結合次氯酸鈣漂白的研究The spores of a. niger m1, the initial strain, was treated by u. v. at morality of 90 %. a mutant with high xylanase activity was screened, naming a. niger j 506
用紫外線照射的方法對出發菌株aspergillusnigerm1進行處理后，從中篩選到了一株木聚糖酶活力比較高的突變株a . nigerj506 ，傳代10次后產酶能力仍然穩定性良好。Study on measuring methods of xylanse activity
木聚糖酶酶活測定方法的探討Application of shearzyme 500l in wheat beer brewing
真菌木聚糖酶在小麥啤酒釀造中的應用Properties of xylanase immobilized on polymer eudragit l
100固定球毛殼黴菌木聚糖酶酶學性質的研究The reduced level of cell - free rumen fluid had no significant effect on xylanase production, but had significant effect on the cmcase activity. without cell - free rumen fluid, the high concentration level of yeast extract could improve xylanase and cmcase production. in the third section, crude enzymes produced by anaerobic fungus a4 was extracted, and their characteristics of the crude enzyme was also investigated
與基礎產酶培養基相比，降低培養基中無細胞瘤胃液濃度對厭氧真菌所產木聚糖酶的酶活及比活力無顯著影響（ p 0 . 05 ） ，但對其所產的羧甲基纖維素酶的酶活及比活力有顯著影響（ p 0 . 05 ） 。Effect of medium components on enzyme production and characterization of anaerobic fungal crude enzymes were also investigated. this thesis was described in the following three sections. in the first section, twelve anaerobic fungal strains isolated from rumen and faeces of ruminants were screened for xylanase and cmcase production
本研究從黑白花種公牛、水牛、山羊糞樣及山羊瘤胃內容物中分離到12株厭氧真菌，並對其進行了產高活性羧甲基纖維素酶和木聚糖酶菌株的篩選，同時還就培養基主要組分對厭氧真菌產酶的影響和厭氧真菌的粗酶性質進行了研究。Condition optimization on complex xylanase by trichoderma viride in solid - state fermentation
綠色木霉復合木聚糖酶的固態發酵條件優化Effect of xylanase on growth and feed digestibility in grass carp ctenopharyngoden idella
木聚糖酶對草魚生長性能和消化率的影響Xylanase refers to a type of enzyme which can hydrolyze xylans into xylooligosaccharides and d - xylose. it broadly exists in microorganism and has wide commerical application in industrial processes, such as feed, paper, foodstuff, medicine and energy industries. the xylanase gene xynb encoding the native protein of xylanase from streptomyces olivaceoviridis a1 was cloned into pichia pastoris expression vector ppic9 a
我們將來源於橄欖綠鏈黴菌a1 （ streptomycesolivaceoviridisa1 ）的木聚糖酶基因xynb的成熟蛋白編碼序列克隆到畢赤酵母表達載體ppic9中，轉化pichiapastorisgs115得到重組子，實現了木聚糖酶基因xynb的高效表達，表達產物能有效分泌且具有正常的生物學活性。分享友人