果占粒 的英文怎麼說
中文拼音 [guǒzhānlì]
果占粒
英文
jam portion-
The results indicate that : 1. the main physical and chemical characteristics vary regularly : with rising of the altitude, there is a transition from silt > sand > clay to sand > silt > clay in the mechanical composition ; the argic horizon emerges below the altitude of 1600 meters ; the content of organic matter is enrichment, the content of organic carbon of epipedon is higher than 20 g / kg, while the content of organic carbon increases with increasing of altitude, and in the altitude of 3500 - 3700meters, the soils under the meadow have the maximum content organic carbon ; the soils appear acid - slightly acid reaction, the ph decreases appreciably and acid strengthen with increasing of altitude ; the soils higher than the altitude of 2500 meters are base unsaturated, indicating the soil leaching is strong, the ph and bs are distinct plus correlated ; the contents of sio2, al2o3, and fe2o3 of the soil body and clay are all relatively stabilization ; in the soil body, the content of sio2 is much high and cao is very little, the total contents of sio2, a12o3 and fe2o3 occupy 92 % of the mineral parts, the sequence of mineral elements is : sio2 > al2o3 > fe2o3 > k2o > mgo > cao > tio2 > mno
研究結果表明： 1太白山南坡土壤的主要理化性質隨海拔高度的上升呈有規律的變化：隨海拔高度上升，機械組成由粉粒砂粒粘粒逐漸過渡到砂粒粉粒粘粒，海拔1600m以下出現粘化層；土壤有機質豐富，表層有機碳含量一般在20g kg以上，有機碳含量隨海拔高度升高而相應增加，海拔3500 3700m的灌叢草甸植被下有機碳含量最高；土壤呈酸性或微酸性，並隨海拔上升， ph值略微降低，酸性增強，海拔2700m以上的土壤多呈鹽基不飽和狀態，表明土壤淋溶作用較強， ph值和鹽基飽和度呈極顯著正相關；土體與粘粒中的sio _ 2 、 al _ 2o _ 3 、 fe _ 2o _ 3含量相對比較穩定，土體中sio _ 2含量較高， cao含量較少， sio _ 2 、 al _ 2o _ 3和fe _ 2o _ 3含量之和約占土壤礦質部分的92 ，礦質元素含量的順序依次為： sio _ 2 al _ 2o _ 3 fe _ 2o _ 3 k _ 2o mgo cao tio _ 2 mno 。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。The chemical components of silkworm pupa crust were analyzed, and its microstructure was characterized by using scanning electron microscope. the existing realtion of among chitin 、 protein and inorganic salt in silkworm pupa crust has been observed. the results show that the major protion of silkworm pupa chitin is in pupa crust, and it accounts for about one forth of crust weight, the out surface of pupa crust is regular polygon net vein characteristics. chitin takes honeycomb shape in chitin - protein complex and conjugated with protein. the inner space of chitin - protein complex net was filled with inorganic salts. thus the theory basis was provided for working out the process route of isolation pupa chitin
對桑蠶蛹皮的成分、結構進行了化學及掃描電鏡分析，確定其含有的主要成分及含甲殼素的數量，並對其中的甲殼素、蛋白質和無機鹽三者之間的存在方式進行了觀察.研究結果表明，蛹體中的甲殼素與灰分主要含在蛹皮中，甲殼素占整個蛹體成分的2 . 71 % ，占蛹皮重量的25 . 5 % ，蛹皮外表面呈規整的多邊形網狀結構，蛹皮中蛋白質與蜂窩狀的甲殼素相結合，呈層狀分佈，顆粒狀的無機鹽填充在甲殼素/蛋白質復合物構成的蜂窩狀的空隙中.這為制定提取蛹甲殼素的工藝路線提供了理論依據A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so
以質粒ppgedna為模板， pcr擴增出1 . 7kb的ge基因完整片段，將擴增產物以ecori和bamhi雙酶切后，插入原核表達載體pbv220的p _ rp _ l啟動子下游的ecori和bamhi位點間，得到重組表達質粒pbvge ，轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后，用sds - page和western - blot ，以及瓊脂雙擴散來檢測，結果表明prvfa株ge基因在原核載體上得到高效表達，表達產物約占總蛋白的17 。The difference at low temperature and the concordance at high temperature still appear. analysis is made and all the results are shown in the form of graph. the result shows the tendency of the critical temperature ' s changing to the given particle number, when the quantum effect is major, is more slow than that got by the integral method
並利用所得能級找出了相互作用系統中粒子的狀態分佈、相互作用對激發態占據數的影響、不同作用下基態占據與溫度的關系以及相互作用對系統轉變溫度的影響，然後用圖形表現出有相互作用時和無相互作用時所得結果的區別。分享友人