果隆多 的英文怎麼說
中文拼音 [guǒlōngduō]
果隆多
英文
golungdo-
In the experiment, in case of the proliferative capability of eg cells, the composition of 0. 25 % trypsin + 0. 04edta was optimal. the growth behavior of icr murine eg cells from pgc was observed
傳代過程中,以0 . 25胰酶與0 . 04 edta聯合作用效果較好,對eg細胞的綜合損傷最小,離散傳代后eg細胞活力比較強, eg克隆出現最多,最高傳至6代。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明:運用msha法檢測1型菌毛的檢出率為80 ( 36 45 ) , pcr法的檢出率為95 . 5 ( 43 45 ) , pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強;選擇5株優勢血清型雞源致病性大腸桿菌代表株( o _ ( 89 ) , o _ ( 119 ) , o _ ( 141 ) , o _ ( 127 ) )的1型菌毛pila基因的pcr擴增片段經純化后,分別定向克隆到puc18質粒的多克隆位點,構建了含有目的基因片段的克隆質粒,並轉化到dh5株大腸桿菌載體菌中,篩選獲得陽性克隆菌株。It adopts the au - labeled monoclonal antibody technology. it has high sensitivity, excel distinctiveness, accurate result and is easy to operate. it widely applies to clinical diagnosis in hospital, self - diagnosis in home, general survey of birth planning and so on
它運用單克隆抗體金標技術研製而成,直接檢測尿液,不需晨尿,具有靈敏度高特異性強操作方便結果清晰等諸多優點,廣泛適用於醫院臨床診斷計劃生育普查及家庭自診等。Scientists like ian wilmut at the roslin research institute near edinburgh, the man who cloned dolly the sheep, has warned that british expertise will go to america, where such work is legal, if the government does not give the go - ahead14
一些科學家,如在愛丁堡附近的羅斯林研究所里曾克隆多莉羊的伊恩?維爾姆特,警告說如果英國政府不批準繼續克隆研究,英國的這項專業技術就會流向美國,因為在美國克隆技術是合法的。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現,抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應;進而通過免疫金標結合電子顯微鏡觀察發現,大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上;統計結果表明,類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中,利用隨機12肽庫對抗豬瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的單抗a11進行表位篩選,經過四輪篩選以後,隨機挑取11個克隆作競爭- elisa檢測,結果表明,所挑11個克隆中,有9個克隆能對me2蛋白和a11反應產生抑制作用,抑制率最高可達64 ; dna測序以後經過dnastar軟體分析,發現它們的核心序列為anwralsl ,該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性;夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別;人工合成含核心序列的多肽經間接elisa試驗證實,也能被a11識別。6, we used gcn5 and rpd3 genes as probes to detect the homologous sequences in drosophila melanogaster by fluorescence in situ hybridization ( fish ). this work has provided useful information for the localization and cloning of related histone acetyltransferase and histone deacetylase genes in drosophila melanogaster
6 ,利用己獲得的酵母gcns和rpd3基因為探針,對果蠅多線染色體進行原位雜交實驗,試圖找出與gcns和rpd3基因同源的基因片段。為今後克隆和分離果蠅中與乙酞化和去乙酚化相關的基因奠定基礎。It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain
通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段,將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較,結果在參比的16個ndv毒株中在該區段中除了有多個點突變外,個別毒株有堿基插入和缺失,所有以lasota株為代表的弱毒株均無6堿基的插入,而以f48e9株為首的強毒株均有此6堿基的插入,但有一個中等毒力的毒株dp沒有6堿基的插入,不過它的基因序列和lasota的幾乎相同,對于所克隆到的基因的代表性還有待確定。Differential screening for the library revealed that, 167 of 237 clones randomly selected from the library are positive and represent genes that were exclusively or intensively expressed in bract, which contain inserts ranging from 100 to 2000 bp in length. moreover, except a few clones were expressed predominantly in bract, absolute majority of subtraclive clones were expressed at low level in all checked organs, i. e., bract, leaf and root
用地高辛標記的珙桐苞片、葉片和根的cdna做探針,與差減雜交所獲得克隆的pcr產物的雜交結果表明,有少數基因在這些器官中都是大量表達,而大多數在這些器官里的表達量均很少,除苞片外,一般均檢測不到,有部分基因在苞片中優勢表達。2. to demonstrate the importance of the consultation exercise, the government made an unprecedented move to set up a " survey office " for the collection and organization of public opinion. it also happened that the survey office also commissioned a commercial market research firm, agb mcnair hong kong ltd, to conduct two large - scale public opinion surveys
為了隆重其事,政府破天荒成立了一個民意匯集處,負責?集和整理市民的意見,及委託一個商業機構agb mcnair hong kong ltd進行兩項大規模的民意調查,最後共收到131 , 589份意見書,及多項民意調查報告和簽名運動結果。The growth speed of clone is different in various inoculating density. the experiment result indicates : lower is inoculating density, faster the speed of growth is
在不同接種密度下,克隆的生長速度不同,實驗結果表明:分瓶數量越多,克隆總重量增長越多。The results showed that the expressions of il - 1 receptor type 6 tt i in normal adult rat brain were very widely, and there were intense immuno - positive cells of il - 1 receptor type i in septal area, medial preoptic area, neocortex, hippocampus, paraventricular nucleus of the hypothalamus, supraoptic nucleus, hypothalamic ventromedial nucleus, arcuate nucleus and median eminence
結果顯示:型il - 1受體在正常成年大鼠腦內有廣泛的表達,隔區、視前內側區、新皮質、海馬、下丘腦室旁核、視上核、下丘腦腹內側核、弓狀核和正中隆起等部位有較多型il - 1受體陽性的細胞。But if engineers can figure out how to integrate millions of relativistic gates on a small silicon chip ? and awschalom is working with research groups at intel and hewlett - packard to do just that ? the result could be processors that run much faster than current models do, while consuming far less power and radiating far less heat
不過假如工程師有辦法把幾百萬個相對論性邏輯閘整合在一小塊矽晶片上,其成果可能就是執行速度比當今機種快很多,而功率消耗與熱輻射卻少得多的微處理器;奧沙隆目前正在和英特爾與惠普的研究群合作研發這種晶片。Beginning form relation of basin - mountain, the tectonic evolution and the petroleum system and pool - forming process have been studied with the integration of structural physics modeling, balance profile reversion and other new testing technology. the main innovative achievements of the dissertation can be summarized as following : 1. on the basis of comprehensive analysis of yanqi basin relationship to tianshan orogenic belts, it was suggested that kuluketage faulted - upheaval was an aulacogen in early paleozoic, which undergone multiple opening - closing along with tianshan orogenic belts, and suffered extensively compressing in late hercyhian cycle and formed a " v - type " thrust - fold belt
論文總的指導思想是以現代石油地質理論為基礎,以整體、動態、系統、綜合分析為原則,以成盆?成烴?成藏研究為主線,運用正、反演相結合的殘留盆地油氣成藏系統評價思路,採用構造物理模擬、平衡剖面復原和多種測試新技術,從盆山耦合關系切入,研究含油氣系統成藏要素及其相互作用過程,探討油氣成藏主控因素和油氣分佈的有序性,取得如下創新性成果和認識: 1 、系統分析了焉耆盆地形成演化與天山構造帶的關系,提出盆地南側的庫魯克塔斷隆在早古生代為一大型裂陷槽,之後隨著天山構造帶的演化,經歷了多次開合運動。Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing
通過序列分析結果表明, jev - ns1基因編碼區核苷酸序列長度為1056bp ,編碼352個氨基酸,序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致,表明盡管該疫苗株在我國應用多年,但ns1基因並未發生變異,提示該疫苗株遺傳性狀比較穩定,是一株優良的疫苗株。Located on neac district, link to the plateau of pomerol petrus, the 45 years vine plants produced a fine wine with natural aromas, long after - taste, very pure and round
此酒來自45年的老葡萄藤,和波爾多頂級寶美隆柏翠斯莊園遙遙想望,出產的酒香氣復雜,帶有紅色水果,蘑菇,麝香,皮革復雜的香味,口感圓潤飽滿,柔美又不失強勁。Cameroon forests contain some of the congo basin most biologically diverse and most threatened forests
其中喀麥隆森林包括了剛果盆地最多的生物物種以及最嚴重的瀕危森林。Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos, and embryonic germ ( eg ) cells from primordial germ cells ( pgc ). both es and eg cells were cultured to determine the factors affecting on isolation, cloning and passage of pluripotent stem cells from icr mouse embryos
試驗以icr小鼠胚胎內細胞團( icm )和原始生殖細胞( pgc )為材料,採用不同培養體系分離克隆胚胎多能幹細胞,探討icr品系小鼠胚胎多能幹細胞分離克隆的基本方法,以及一些因素對其分離、培養、克隆、傳代等效果的影響。Results there are wide applications for medicinal plant researches, such as identification, genetic relationship, origin and evolution, genetic diversity, gene mapping, gene cloning and genetic engineering of metabolic pathway
結果分子標記在藥用植物的鑒定、親緣關系、起源進化、遺傳多樣性、基因定位及克隆、代謝途徑的基因工程研究等方面有廣泛的應用空間。However, some of face recognition problems still require further development, this is the case for problems of recognition face images conveying changes in illumination, facial expression and changes due to the time delay between the acquistion of the reference and tested face images. our main work is to analysis methods of extraction face features and contraction of classifier. the work presented in this paper is to apply self - organizing feature map and minor component to extraction features from multi - view face images, then combine those features as a new combined feature set, in order to reduce redundancy data, we apply clone algorithms to reduce data through rotation in input space
我們改進了一種基於矩理論的識別方法,給出了計算公式和證明過程,可用於解決小規模人臉識別問題;我們將智能方法應用到人臉識別中,分別利用自組織特徵映射和次分量方法抽取人臉的整體特徵和局部特徵,依據特徵融合理論,重新組合為新的復合特徵,為壓縮特徵數據,我們首次引入克隆選擇演算法自動進行特徵優化選擇,最後,利用支持矢量機構造多分類器進行分類識別,在不同規模人臉識別庫上模擬結果表明,該系統自適應能力強,分類識別精度高,適用於大規模復雜人臉識別問題。分享友人