柱上變電所 的英文怎麼說
中文拼音 [zhùshàngbiàndiànsuǒ]
柱上變電所
英文
polemounted substationn-
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質The solving steps are as follows : the field is divided into three regions by the cylindrical surface of the finite - length cylinder and the location of the delta - coils. because " the surface current source is placed at the interface, the non - homogenous restricted equation on the magnetic vector potential is transformed into the homogenous helmholtz equation. by using the method of separation of variables to solve equation and according to the interface conditions and the condition at infinity to determine the unknown constants, the expressions for the magnetic vector potential of the three regions are solved out
在求解過程中，假定放置式圓柱線圈是由無窮多個對稱圓環線圈密饒而成，首先求解含有有限長磁芯的通電對稱圓環線圈的電磁場：以有限長圓柱磁芯的側面和圓環線圈所在圓柱面為分界面將場域劃分為三個小區域，由於場源放置在內邊界面上，使得關于矢量磁位的非齊次約束方程轉化成齊次亥姆霍茲方程，利用分離變量法求解，根據分界面鄭州大學碩十研究生畢業論文摘要條件以及無限遠條件確定待定常數，從而得到各場區矢量磁位的表達式。
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