柱層析 的英文怎麼說

中文拼音 [zhùcéngxī]
柱層析 英文
column chromatography
  • 層 : i 量詞1 （用於重疊、積累的東西 如樓層、階層、地層） storey; tier; stratum 2 （用於可以分項分步的...
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  1. Studies on community structure of ciliates from activated sludge in yanerwan sewage plant in spring

    採用常壓柱層析及薄層層析從大花翠雀
  2. Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method. at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds - page

    將粗酶液過deae -瓊脂糖柱層析，得三個活力峰，分別收集、透析、乾燥濃縮后；再上sephacryls - 200凝膠柱層析，按與deae -瓊脂糖柱層析后同樣的方法收集處理。
  3. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  4. This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column

    使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。
  5. According to the polarity, the residue was isolated with petroleum ether, chloroform, ethyl acetate, and n - buoh, respectively. the n - buoh fraction, confirmed as neuroactive component, was subjected to sephadex lh - 20 column chromatography to provide an extract fraction, as a buff powder, which could induce neurite outgrowth in rat pheochromocytoma pc12 cells in a dose - dependent manner up to 50 mg / l

    將菟絲子乾粉用75乙醇浸泡后，減壓蒸干后得到褐色漿狀物，經石油醚、氯仿、乙酸乙酯、正丁醇萃取，經柱層析后，再用葡聚糖凝膠對有效成份進一步純化，獲得了菟絲子中能誘導pc12細胞分化的活性組分。
  6. The active agent was confirmed as oligosaccharide, mostly composed of glucose by pc, vc and acidhydrolysis. the active agent is stable between ph2 - 12 and below 90c

    通過紙層析（ pc ）和柱層析（ vc ）分析，活性組分酸水解分析初步證實了其組分為葡萄糖為主要成分組成的寡糖類物質。
  7. Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others

    通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。
  8. Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase

    3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。
  9. Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography

    摘要通過兩次離子交換柱層析，從牛腦中制備鈣調素依賴性的環核苷酸磷酸二酯酶。
  10. Mm ). mg2 +, mn2 +, zn2 +, fe2 +, fe3 +, cu2 + have enabled effect on enzyme activation and edta produce a strong inhibitory effect on the enzyme. embranch amino acid have no effect on the enzyme

    在離子交換柱層析中，採用不同的ph值及不同類型的緩沖液對純化條件進行優化，最終選擇了ph6 . 0mes緩沖液，並得到了酶蛋白洗脫點為0 . 24 0 . 32mol lnacl 。
  11. Methylbenzyl amine as chiral auxiliaries to react with racimic ketorolac. then authors got optical amides. the diastereoisomeric mixture were separated by column - chroatography on silica gel. the results of separation depend on the structure of r

    苯乙胺作為手性輔基，與外消旋酮咯酸生成一對光學活性的酰胺衍生物，再用簡單易行的柱層析方法分離其非對映異構體。
  12. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  13. When being incubated at 60, 70, 80, chlase could lose its half activities in 21 minutes, 22 minutes, 18 minutes. with different chl, chlase showed different kinetic properties. different ions were found having different effects on chlase activities

    以菠菜為材料，分離了pao活力檢測所需的還原性劑fd ，經丙酮沉澱、透析脫鹽、 deae離子交換柱層析等純化步驟進行了純化， fd純化了6 . 6倍。
  14. - acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation, 50, 2min heat treatment and deae - sepharose fast flow column chromatography, which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6

    對其酶學性質進行了研究。 -乙酰乳酸脫羧酶經50 80硫酸銨分級沉澱、 50 ， 2min熱處理、 deae - sepharosefastflow離子交換柱層析方法分離純化。
  15. - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography

    本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。
  16. Results galangin was obtained by on - step column chromatography of polyamide only once

    結果運用聚酰胺柱層析次分離，獲得有效成分高良姜素。
  17. A white needle crystal was isolated and purified on systematic extraction of the alkaloid by silica gel column and gradient sublimation from oxytropis glabra

    摘要採用生物?系統提取、矽膠柱層析分離及梯度升華技術從小花棘豆乾草粉中分離出一種白色針狀結晶。
  18. The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane

    雙胸蚓組織中dna酶的分離純化雙胸蚓組織粗提取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素（ de52 ）柱層析、超濾膜分級分離后得到一個電泳純的dna酶。
  19. Many kinds of chromatograph including silica gel, d101 resin, sephadex l - 20 gel, rp - 8 and hplc are used in extracting and separating chemical constituents form these plants. certainly, they need to select the best eluate by tlc. some technique including ms ( ei - ms, fab - ms ), id and 2d nmr ( cosy, roesy, hmbc, hmqc ) been used in identifying these chemical structures

    通過各種硅膠柱層析，吸附樹脂， sephadex系列凝膠樹脂以及rp - 8類反向柱等層析材料以及高效液相色譜等技術，利用不同的洗脫體系對這兩種植物的化學成分進行分離得到純的單體化合物，然後利用ms （ ei - ms ， fab - ms ） 、一維nmr 、二維nmr （ cosy ， roesy ， hmbc ， hmqc ）等技術對這些化合物進行結構鑒定，利用ir和uv對這些化合物進行了表徵。
  20. The bioactive strain ' s fermentation product was isolated and purified primarily using methods of solvent extraction, acid - alkali extraction, ab - 8 macropore absorption chromatography, 1 x 007 positive ion exchange chromatography, the result showed the purification product has prominent bioactivity inhibit staphalococcus aureus

    中國熱帶農業科學院、華南熱帶農業大學2003屆碩士研究生採用有機劑抽提法、酸堿抽提法、大孔吸附樹脂柱層析和陽離子交換樹脂柱層析等方法對hsl 306菌株發酵產物進行了初步分離純化。
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