核刺激法 的英文怎麼說
中文拼音 [hécìjīfǎ]
核刺激法
英文
nuclear stimulation-
This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。The therapy also resulted in a decrease in the severity of rigidity and bradykinesia extreme slowness of movement but not tremors, reduction of depression, and improvement of motor function. other therapeutic modalities using of " light " include visible light therapy for the treatment of acute or semi - acute eczema, tumors, dermatitis, and infant jaundice ; ultra - violet therapy for disinfection and sterilization ; laser therapy used in operations, heat therapy, photon - pressure therapy and acupuncture ; photochemical therapy in the treatment of skin diseases ; etc.
其它與光有關的醫療保健方法還有：可見光療法visible light therapy ，可用於治療急性或亞急性濕疹疤痕疙瘩皮膚炎新生兒核黃疸等紫外線療法ultra - violet therapy可用於創面照射殺菌和空氣消毒激光療法laser therapy用於手術熱療光壓療法及針灸時用於穴位上可代替針刺光化學療法photochemical therapy可用於治療某些皮膚病等12 。Immunohistochemistry method was used to observe the temporal and spatial expression of nmdar2, signal molecules, skeleton proteins and connexins in son neurons and glias ( astrocytes and microglias ). radioimmunoassay was used to detect vasopressin ( vp ) content in plasma before and after hyperosmotic stimulation. ultrastructure between activated son astrocytes and neurons was observed by double immune - electron - microscopic staining method
應用免疫組織化學方法光鏡下觀察高滲刺激后，大鼠視上核膠質細胞（星形膠質細胞和小膠質細胞）受體（ nmdar2 ） 、信號分子、骨架蛋白及縫隙連接蛋白的表達的時空變化；應用放免測定檢測高滲刺激前後血漿中vp含量。These include inflammatory stimulation by interaction of alpha - synuclein with microglia and astrocytes and a suppressive action by nonsteroidal anti - inflammatory drugs on dopamine quinone formation
這包括阿爾法-突觸核蛋白與小膠質細胞和星形膠質細胞相互作用產生的炎癥性刺激，以及非甾體類抗炎藥對多巴醌形成的抑制作用。Design : viable mtb were detected in mesenteric lymph nodes, spleen and lungs at day 15, 30and 60 after intragastric administration of bcg to mice : the proliferation of t - lymphocyte with the stimulus of the purified protein derivative ( ppd ) was measured at day 60 and 90 after intragastric administration of bcg to mice ; the expression of interleukin - 2 ( il - 2 ) of t - lymphocyte in spleen was detected at day 60 and 90 after intragastric administration of bcg
方法：在小鼠胃內接種bcg的第15 ， 30 ， 60天，檢測結核桿菌在小鼠體內臟器的定植；接種bcg的第60 ， 90天，檢測小鼠脾淋巴細胞受到ppd刺激后轉化試驗小鼠脾淋巴細胞受到ppd刺激后il - 2表達量。A eukaryotic expression vector pcdna3. 1 - cptl was constructed by insert cp77 gene into the vector pcdna3. 1 which is used in nucleic acid immunization. the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal. immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body
本研究通過限制酶將cpti基因片段從載體pbluel3上切下，插入真核表達載體pcdna3 . 1 ，構建了用於核酸免疫的真核表達載體pcdna3 . 1 - cpti ；質粒大量提取和純化后，通過肌肉注射的方法免疫balb c小鼠，基因表達產物刺激小鼠機體產生免疫反應，從而獲得了抗cpti蛋白的抗體。分享友人