核原生質體 的英文怎麼說
中文拼音 [héyuánshēngzhítǐ]
核原生質體
英文
karyoplast-
Based on the frequent occurrence of international aerial crimes, the rampancy and cruelty of criminals and the reality of brutal consequence, the essay analyses the features of the crime of aircraft hijacking, the crime of imperiling international civil aerial security and the crime of obstructing international aerial navigation with the component terms from three important international conventions on punishing aerial crimes in order to help people to appreciate the appearance and the essence of typical international aerial crimes deeply, to regard the motion of the crimes and to overwhelm the offence forcefully. based on the core of the legislative status of international civil aerial security, the jurisdiction of the aerial crimes, one emphasis of the article is to clarify concrete clauses on jurisdiction in the three conventions and analyses the important principle of " aut reddere, aut punire ". based on the problems on the jurisdiction of crimes in international society, it quests the implementation of t he principles of jurisdiction, pleading and extradition in the three conventions deeply
基於目前國際社會航空犯罪時有發生，犯罪分子猖獗狠毒，危害後果十分殘重的現實，本文從國際社會關于懲治航空犯罪的三個重要國際公約（東京、海牙、蒙特利爾公約）中規定的各種航空犯罪的定義入手，深入展開了對劫持航空器罪、危害航空器飛行安全罪、危害國際民用航空機場安全罪的犯罪特徵及其構成條件的分析，以期幫助人們更深刻認識這些典型的國際航空犯罪的表現及其實質，嚴密注視這些犯罪的動向，對其進行及時有力的打擊；鑒于國際民用航空安全法律地位問題的核心是解決航空犯罪的管轄權問題，本文重點闡釋了三個公約關于管轄的具體規定，並側重分析了「或引渡或起訴」的重要原則；鑒于目前國際社會打擊國際航空犯罪管轄方面存在諸多實際問題，本文對公約規定的管轄、起訴、引渡原則的實現方式作了深入探討；鑒于各國在國內立法上對于空中犯罪的懲治、對違約國的制裁、對這類犯罪的預防措施等方面存在一些漏洞，本文根據目前掌握的一些資料，在獨立思考的基礎上，提出了一些不成熟的看法及建議，以期對國際社會懲治航空犯罪貢獻自己微薄之力。The result shows that the life cycle of all the species comprises a unicellular amoeboid or swarm cell stage, a multinucleate plasmodium stage and a sporulation stage
結果表明，生活循環均包括單核的黏變形體或游動胞、多核的營養體原質團以及形成孢子階段。Prokaryotes lack organelles such as nucleoli, mitochondria, plastids, golgi apparatus, and do not exhibit cyclosis
原核生物無細胞器，如核仁、線粒體、質體、高爾基體等，不出現胞質環流。The primary cell propagated 8 times and were small fusiform or triangle and smaller than hepatocytes with larger nucleus and less plasma ; it was 88. 2 % in the growing period of 4 ( superscript th ) generation cells and the stains of alb and ck19 were positive in immunocytochemical
所獲得的原代細胞共傳8代，細胞呈小梭形或三角形，體積較小，胞核較大，而胞漿較少；第4代細胞中進入生長期的細胞約佔88 . 2 % ；免疫細胞化學染色顯示細胞胞質中alb 、 ck19染色陽性。After entry of wto, there are still many maladjustments in guangxi ' s seed industry, such as the weak foundation of sees industry development ; the unformed market of the seed industry for fair competition ; small - scale seed enterprises ; no systematic connection among the cultivation, breeding and marketing ; lower qualification of staff who work in the seed industry and with weak sense of laws, and lack of the knowledge in operating experiences in the international market and trade etc. yet the un - efficiency system, unclear property right in enterprises, the lack of an effective mechanism to promote the rational use of resources in the seed industry and the lack of such concept as " the government creates environment and enterprises create fortune " are the deep - seated causes of the problems in guangxi ' s seed industry. therefore, the key points for promoting development of guangxi ' s seed industry under the wto framework are to focus on the promotion of the developing capability of seeds " integrated products, constantly deepen reforms, to adjust various relevant factors in the system of the seed industry which is inconsistent with each other, and to establish a new - pattern system with evident characteristics of the time spirit in order to meet the requirements of the market economy. hereinto, the specific strategies and measures for promotion of guangxi ' s seed industry development under the wto framework include kee ping up reform and innovation of the system of the seed industry, executing of non - nationalization reform in state - owned seed enterprises, formulating and executing relevant supporting policies, the improving the legal system in the seed industry, increasing public financial support on the seed industry, promoting the integration of cultivation, breeding and marketing, strengthening human resource development, developing the main body of the seed industry ' s market and making proper conditions for the functions of seed associations in the seed industry development etc
研究結果認為：發展種子產業應該把著眼點放在促進種子整體產品的開發上；種子產業的發展依賴于能充分發揮整體功能的新型種業體系的構建，而目前廣西種業體系中的品種選育、種子生產加工、種子經營以及政府管理、公共支持和社會服務六個主要組成部分都存在明顯的缺陷與不足；廣西種子企業綜合競爭力總體處于較弱水平；面對wto ，廣西種子產業仍有諸多的不適應，突出表現在產業發展基礎薄弱、尚未形成可以公平競爭的種業市場、種子企業規模小、育繁銷脫節、種業人才素質不高、種子企業法律意識淡薄、國際市場運作經驗和國際貿易知識不足等多個方面，而體制不順、企業產權不明晰、缺乏促進種業資源合理流動的有效機制以及「政府創造環境，企業創造財富」的正確理念正是導致目前廣西種子產業不能適應入世需要的深層次原因；因此，以提升種子整體產品開發能力為核心，不斷深化改革，調整種業體系中不相協調的各有關因素，構建起符合市場經濟體制要求的具有鮮明時代特徵的新型種業體系，是wto框架下加快廣西種子產業發展必須堅持的指導思想；其中，加快種業體制改革和創新、實施對國有種子企業的非國有化改造、制訂落實有關扶持政策、完善種子法律法規體系、加大公共財政對種子產業的支持力度、推進育繁銷一體化的形成、加強人才培養與引進、壯大種業市場主體、實行重點突破戰略、發揮種子行業協會作用等等，都是wto框架下加快廣西種子產業發展應該採取的具體對策措施。Protoplasted monokapyon ( pm ) were prepared from lentinus edodes strains jingxuan and wuxiang, respectively, intercross process were put in practice
摘要以香菇菌株精選和武香作為親本制備單核原生質體，將兩個親本的單核原生質體進行一一雜交。On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection
本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。A specific cis - hairpin ribozyme facilitates the infection in tobacco protoplasts
一個特殊的順式發夾核酶對感染煙草原生質體的影響Such examples are given as the blood filtering principal of hemoglobin, catalysis of enzymes, immune recoglization, prion, glycoprotein and the relationship of structure and function of membrane protein, et al, as well as the applications to medicine
在簡要介紹結構生物學的研究方法的基礎上，主要從分子水平闡述蛋白質和核酸的結構原理、相互作用、結構與功能的關系，通過具體實例闡述血紅蛋白的輸氧機制、酶的催化機制、免疫分子識別、朊病毒、糖蛋白、生物膜的結構功能關系等，以及結構生物學在醫學上的應用。Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system
人骨形成蛋白3 （ hbmp - 3 ）屬于tgf -超家族的一員，可以誘導間充質細胞分化為軟骨和骨，在胚胎時期骨骼發育和骨再生修復中起著重要的作用，而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用，因而hbmp - 3有廣闊的市場前景。它在人體內含量極微，盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達，但是由於原核表達系統缺乏翻譯后的加工修飾，真核表達系統存在成本高、產量低等特點，限制了其在臨床上的應用。Accounting for contingencies is one important part of uncertainty accounting ， contingencies widespread exist in enterprises ， as a special economic event ， with developments of economic ， financial relations is complex ， the contents of contingencies unceasing extend ， contingencies plays a more and more important role in financial accounting information and operation policy because theoretical and practical research of contingencies is late ， special data is little ， few pay a ~ ention to treatment of contingencies new accounting law points out that enterprises must explain contingencies arising from guarantees provided for the debit of other enterprises and pending litigation this is the first time that the law requests accounting treatment of contingencies accounting standard for business enterprises ： contingencies prescribes the recognition and measurement of contingencies and the disclosure of relevant information ， the standard is the first standard of contingencies ， improves the accounting treatment for contingencies and the disclosure of relevant information the standard will help us improve the quality of information disclosure the article is divided into five parts to discuss the first part is a general introduction of contingencies ， including the history, the classification ， the framework and so on ， all of which would help readers understand the basic concepts in the article the second part introduces recognition of contingencies ， including recognition condition and accounting of some contingencies etc the third part introduces measurement of contingencies ， including measurement principle and selection of measurement attributes the forth part introduces disclosure of contingencies ， including disclosure of contingent asset ， contingent liability etc the fifth part selects some enterprises financial report to analyse ， points out some problems of contingent disclosure ， gives some suggestions about disclosure of contingencies
對于規范我國企業或有事項的會計核算和相關信息的披露問題，提高會計信息披露質量，保護投資者利益，具有重要的現實意義。本文試就或有事項的確認、計量以及信息披露略談一下自己的認識，對準則可能存在的不足之處提出意見和建議，並採用實證方法對上市公司或有事項的披露狀況進行了分析。本文共分五章進行論述，第一章是對或有事項的概括性介紹，包括或有事項的涵義、特徵、或有事項會計的產生、或有事項的分類以及或有事項會計的基本框架等，有助於讀者了解本文的一些基本概念；第二部分介紹了或有事項的確認，介紹了或有事項的確認條件和幾種具體或有事項的會計處理；第三部分介紹了或有事項的計量，包括或有事項的計量原則和計量屬性的選擇等問題；第四部分介紹了或有事項的披露，包括或有資產、或有負債、預計負債等的披露；第五部分選取了部分上市公司的年報進行實證分析，指出了我國上市公司在或有事項披露方面存在的問題，並提出了完善我國上市公司或有事項信息披露的建議。Therefore the emergence and development of the culture of science and technology can be traced back to the initial conditions of geography and climate, viewed as the factor of internal fluctuation from the sensitiveness of the random choices of the cognitive subject to the initial conditions. since a culture has a pivotal component as its core, once the culture of science and technology comes into being under nonlinear interactions of the social subsystems, the core component functions in the same manner as order parameter in the self - organization system, immensely attracting, regulating and controlling others under the slaving principle. and thus the analysis provides a nonlinear approach to the needham ' s problem
對于任何思想文化傳統而言，其形成和延續都有其現實的物質生活的根源，因而，對于科技文化的產生和發展，我們能夠返回到其發生的原點? ?即地理氣候等初始條件，從認知主體的隨機性選擇對初始狀態的敏感性方面，視其為科技文化產生的內漲落因素，考察其對科技文化形成的影響；而一種文化之所以稱其為文化，必有其全局控制力的核心因素，科技文化是在社會各子系統非線性的相互作用下形成，其核心因素所發揮的作用就如同序參量在自組織系統中的作用那樣，在支配原理的作用下起著規范、引導和支配等效用。Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully
本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。Using the microwave selective heating property for materials, by setup equivalent equation, and first time inducing the electromagnetic field perturbation theory to the design of heating materials for substrate in mpcvd, three temperature distribution modes were established, including temperature distribution comprehensive mode of inhomogeneous plasma, temperature distribution composite mode of composite substrate materials, temperature distribution perturbation mode of composite materials, which ii provided an whole new technology route to the design of substrate heating system in mpcvd and guided the preparation of heating materials for substrate. and then the heating materials for substrate were designed and optimized to obtain large area homogeneous temperature distribution even larger than substrate holder ' s diameter. as an important part, this thesis researched the nucleation and growth of diamond films in mpcvd, systematically researched the effects of substrate pretreatment, methane concentration, deposition pressure and substrate temperature etc experimental technologic parameters on diamond films " quality on ( 100 ) single crystal silicon substrate in the process of mpcvd, characterized the films qualities in laser raman spectra ( raman ), x - ray diffraction ( xrd ), scanning electron microscopy ( sem ), infrared transmission spectra ( ir ), atomic force microscopy ( afm ), determined the optimum parameters for mpcvd high quality diamond in the mpcvd - 4 mode system
該系統可通過沉積參數的精確控制，以控制沉積過程，減少金剛石膜生長過程中的缺陷，並採用光纖光譜儀檢測分析等離子體的可見光光譜以監測微波等離體化學氣相沉積過程；利用微波對材料的選擇加熱特性，通過構造等效方程，並首次將電磁場攝動理論引入到mpcvd的基片加熱材料的設計中，建立了非均勻等離子體溫度場綜合模型、復合介質基片材料的復合溫度場模型及復合介質材料溫度場攝動模型，為mpcvd的基片加熱系統設計提供了一條全新的技術路線以指導基片加熱材料的制備，並對基片加熱材料進行了設計和優選，以獲取大面積均勻的溫度場區，甚至獲得大於基片臺尺寸的均勻溫度區；作為研究重點之一，開展了微波等離體化學氣相沉積金剛石的成核與生長研究，系統地研究了在（ 100 ）單晶硅基片上mpcvd沉積金剛石膜的實驗過程中，基片預處理、甲烷濃度、沉積氣壓、基體溫度等不同實驗工藝參數對金剛石薄膜質量的影響，分別用raman光譜、 x射線衍射（ xrd ） 、掃描電鏡（ sem ） 、紅外透射光譜（ ir ） 、原子力顯微鏡（ afm ）對薄膜進行了表徵，確立了該系統上mpcvd金剛石膜的最佳的實驗工藝參數。The most popular idea proposes that solid cores amass from protoplanetary disks and behave like seeds, attracting gas to grow into giant planets
最流行的學說倡議：固態的行星核心由原生行星盤獲取質量，就像一顆種子，能吸引氣體長成為巨行星。Applications of animal ' s growth hormone are mostly studied by means of protein type, up to now, no other reports about gh gene directly used in animals have been found except one paper about the transfection of human gh gene into mice. in this research, we studied the changes in the bullfrogs after they are separately injected with the recombinant bullfrog gh protein ( re - bfgh ), bullfrog gh plasmid ( vb / gh ), grass carp gh plasmid ( vgcgh ) and expression vector vr1020 while the 0. 85 % salt - water as the control, for the purpose to determine the possibility of that the eukaryotic expression plasmid vbfgh and vgcgh are expressed in adult bullfrogs and affect their growth rate and plasma gh. we hope the results will help developing a new approach to promote the animal growth
本研究首次以兩棲動物牛蛙為研究對象，進行了其生長激素基因的克隆以及原核表達質粒與真核表達重組質粒的構建、重組牛蛙gh蛋白的生物活性和免疫活性檢測以及重組蛋白制劑和核酸制劑的制備及其體內促長作用等表達效應研究，研究目的在於求證重組真核表達質粒是否能在牛蛙中表達、其促長效應是否強于重組bfgh蛋白，為探索重組生長激素真核表達質粒能否替代重組gh蛋白作為動物促長基因制劑等研究奠定理論基礎。In the first of 1990s, a new kind of vaccine - dna vaccine gives a new way to solve the immunogenicity of cea. it is defined as plasmids encoding antigen protein which can elicit immune responses against the antigen through direct injecting into body and taken into by cells, then, it stimulates the body generate specific immune response through different routes
核酸疫苗是指將含有編碼腫瘤相關抗原基因的真核表達質粒，直接接種到動物體內，然後被動物細胞攝取並表達出相應的抗原，通過不同途徑刺激機體產生針對該抗原的免疫應答，從而消除或抑制腫瘤細胞的生長。3. characteristics to point out the essence of opportunity cost is return rate of factors, so use the opportunity cost of factors to do the benefit - cost account of agriculture products is in line with the principle of market - economy. to suggest improving the ways of the benefit - cost account of agriculture products, and put forward a new system of he benefit - cost account of agriculture products ; to calculate the shadow prices of agriculture production factors by using stochastic frontier production function and marginal productivity theory, especially acquire the shadow prices of the agriculture land ; to do the principle analysis on the supply - demand mechanism, and suggest the supply and demand curves of china agriculture production factors ; to prove the applicability of using stochastic frontier production function to research the product ion cost of agriculture products
本文研究的創新和特色之處為：指出要素機會成本的實質是要素的投資報酬率，從而認為以要素機會成本作為農產品成本收益核算的基礎概念符合市場經濟原則；提出以生產要素的機會成本概念為計價原則，以改進中國農產品的成本收益核算方式，據此提出符合市場經濟原則、體現完全生產成本的中國農產品成本收益核算項目體系；以隨機前沿生產函數方法和要素邊際生產力理論為邏輯框架直接測算中國主要農業生產要素的價格，特別時土地要素的價格；對中國農業生產要素的供給和需求機制進行理論分析，給出中國農業生產要素的供給、需求曲線；在理論上論證用隨機前沿生產函數技術研究農產品生產成本問題的適用性。分享友人