核定位序列 的英文怎麼說
中文拼音 [hédìngwèixùliè]
核定位序列
英文
nuclear localization sequence nls-
G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc
當分別共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時，都觀察到gfp和hochest33342標記的轉染后hela細胞表現多核和染色質橋現象。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Meanwhile, these six coat protein genes were sequenced and compared with other homologous sequences in genbank. the strain designation of the six isolates of tumv was finally determined. the results are as the following : 1. six isolates of turnip mosaic virus named tumv - sd1, tumv - sd2, tumv - sd3, tumv - sd4, tumv - sd5 and tumv - sd6 were respectively acquired from infected chinese cabbages and radishes in 3 cities ( taian, yantai and zaozhuang ) of shandong province
利用rt - pcr方法克隆了tumv山東分離物的外殼蛋白（ cp ）基因，測定了它們的核苷酸序列、並將其與已報道的序列進行同源性比較和分析，最終確定了其歸屬地位，具體研究結果如下： 1 .從山東省3地市感病的白菜和蘿卜上分離到蕪菁花葉病毒的6個分離物，分別命名為tumv - sd1 、 tumv - sd2 、 tumv - sd3 、 tumv - sd4 、 tumv - sd5和tumv - sd6 。( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
（ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。Identification of znf219 nuclear localization signals
219基因核定位信號序列的確定In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。At the same time, the c - terminal expressing plasmids of hcap - e and hcap - c that attached the kozac sequence and the nuclear localization signal were also constructed
同時也構建了附加kozac序列和核定位信號的hcap - e和hcap - c的c -末端真核表達質粒。To identify the transfected cells, we also constructed the gfp expressing plasmids which also attached the kozac sequence and nuclear localization signal to the gfp gene and the plasmids served as a selective marker for the transfected cells
為了區分被轉染的細胞，以附加kozac序列和核定位信號的gfp （ pcdna3 . 1 + kg ）作為被轉染的細胞的標記。In our studies, we have used the nuclear localization signal department of medical genetics and development biology 4 ( nls ) selection system to isolate a novel gene from a mouse embryonic cdna library. the full - length cdna is about 1802bp and contains an open reading frame of 1296 nucleotides. it encodes 431 amino acid
本研究工作，利用本室構建的核定位信號篩選系統從小鼠胚胎cdna文庫中克隆到一個新的全長cdna片段，分析表明在其1802bp育回軍區大學刃士學位論文的序列中含有一個長1293hp的開放閱讀框，編碼431個氨基酸。This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv
為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。The shrna based on telomerase htert gene 1573 - 1591 sequence can silence hela cell telomerase gene expression in a detectable extent
以端粒酶tert基因1573 ? 1591位的核酸序列構建的shrna可對hela細胞端粒酶基因表達產生一定的沉寂作用。On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction
本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。At the same time, vp4 gene was mutated in a certain point by pcr. the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly. a. tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l. guad according to the leaf disc procedure
為便於基因操作，對外殼抗原蛋白vp4基因進行適當的修飾和改造：通過引物設計，利用pcr反應，在基因的起始編碼前引入有助於真核生物表達的kozak序列和限制性內切酶位點；使用套疊pcr對vp4基因進行定點突變，以便於將改造后的基因插入pbi121構建植物表達載體，通過直接轉化法，把pbi121 vp4轉入農桿菌eha105 ，構建了農桿菌工程菌。By rt - pcr method. we first selected a pair of primers named 2bp and 4bm which was designed in highly conserved 922 nucleotide region in open reading fram ( orf ) ib from coronavirus. this primer could amplify 11 kinds of coronaviruses. after synthesizing this pair of primers, we amplified the target fragement of 251bp from the panda ' s liver - tissue materials and other different passages of culture of this virus. however, the control cell showed negative result
第一對引物為外層引物，可擴增出1086bp的核酸片段；第二對引物為內層引物，可擴增出515bp的核酸片段。該引物以k378為參考株，含有多個兼并堿基，可擴增出包括k378 、 insavc - 1 、 ccv1 - 71 、 nvsl吉林農業大學碩士學位論文大熊貓犬冠狀病毒的分離鑒定及s墓因部分序列的測定和分析等多種犬冠狀病毒。A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。They are as follows : job analyse and post manual ; location, organization, implement procedures ; assess criteria, result exertion of performance appraisal ; principles, mode and structure of the compensation system ; relative standard of wages and salaries ; concrete scheme of the wages and salaries for all position series ; and the long - term incentive payment plan for senior executives and key backbones
設計方案涉及了工作分析與崗位說明書的編制；績效考核的定位、管理組織、實施程序、考核標準及考核結果的處置；薪酬體系的指導思想與原則、模式與結構；薪資的相對標準；各系列崗位薪酬的具體方案；高管人員與核心骨幹的長期激勵計劃。And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。In order to construct the recombinant plasmid pcdna3. 1 / ts87, first, the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i, and kozak sequence. then the rebuilt ts87 fragment was cloned into t - vector and transformed into e. coli dh5 a
通過重新設計引物在ts87兩端加上用於構建重組質粒的酶切位點hind和bamh和用於真核表達的kozak序列。將改造后的基因片段克隆入t - vector ，轉化大腸桿菌dh5 ，通過藍白斑篩選獲得陽性克隆后測序鑒定。To explore the feasibility of ib edible vaccine, we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product. the findings are as follows : 1. a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121
為了探索ibv可食性疫苗的可行性，我們進行了轉基因馬鈴薯表達ibv免疫原基因及其表達產物免疫原性的研究，取得以下結果： 1根據周繼勇等報道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表達載體的多克隆位點，設計併合成引物，以含s1pbs質粒為模板擴增s1基因，將擴增片段定向克隆到pbi121質粒的35s啟動子下游。分享友人