核染質粒體 的英文怎麼說
中文拼音 [hérǎnzhílìtǐ]
核染質粒體
英文
chromatin granule-
At low concentration of zn, the changes in ultrastructure were nuclei deformation, chloroplast swelling and disorder of thylakoid arrangement ; serious damages in ultrastructure caused by greater zn stress were indicated by scattered nucleoli, condensed chromatin, almost empty nuclei with nuclear membrane disrupted and nucleoplasm flowing into cytoplasm, swollen and partly dissolved cristae of mitochondria, disrupted and collapsed chloroplast envelopes, and some dissolved thylakoids that flew into cytoplasm
超微結構的變化也呈現加重趨勢，低濃度處理的變化為細胞核變形、葉綠體膨脹、類囊體排列紊亂；嚴重的超微結構的損傷是核仁散開、染色質凝集，細胞核幾乎成為空核和核膜破裂，核質散出；線粒體脊突膨脹和部分溶解；葉綠體膜斷裂、消失和部分類囊體溶解和散到細胞質中。During the stages of primary and secondary spermatogonium, components of the nucleolus, called chromatoid bodies ( cb ), are excluded from the nucleus
精原細胞階段，部分核仁物質外排，成為擬染色質小體，其上聚集一群線粒體，構成「線粒體區」 。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization
端粒酶（ telomerase ）是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物（ rnp ） 。端粒酶含有引物特異識別位點，能以自身rna為模板，逆轉錄合成端粒dna並加到染色體末端，使端粒延長，從而延長細胞的壽命甚至使其永生化。Plasmids are exta-chromosomal doublestranded, circular dna molecules found in prokaryotic and eukaryotic cells.
質粒是在原核和真核細胞中發現的染色體外的雙鏈環狀DNA分子。Four different types of connective tissue cells are found out of the basal lamina, hi the first type of connective tissue cells, most of the nuclei are occupied by normal chromatins, but in other three types of connective tissue cells, abnormal chromatins are rich in nuclei
4 、結締組織細胞與基膜相連，主要有4類。第1類細胞：細胞核中常染色質居多，而其它3類細胞核內異染色質較多。第2類細胞：細胞質內含有許多體積較小的電子緻密顆粒。The obtained recombinant - 5 - htr plasmid was tranfected into human liver cancer smmc - 7721 cells. all data suggested the expression of plncx - atr could condense cell nucleus and increase nuclear fluorescence intensity, effectively repress the telomerase activity, cell growth and cell proliferation, and induce cell apoptosis
反義重組質粒plncx - atr轉染人肝癌smmc - 7721細胞，結果發現plncx - atr的表達有效地封閉或抑制肝癌細胞的端粒酶活性，使細胞的生長和增殖受到抑制，細胞體積變小、核緻密、核熒光強度增強，且促進其凋亡。Results showed : ( 1 ) cbt cell death in low tempratures is accompanied by characteristic changes, such as, reduced cell size, distorted nucleus, chromatin condensation and margination and cell ( cytoplasmic ) vacuolization ; cell mortality and ca2 * concentration increase along with time passed in low temperature. mitochondrial membrane potential and 02 increased at first, and then decreased. activities of sod decreased at first, followed by significant increasing and finally depressed
結果表明： （ 1 ） cbt在低溫協迫下，細胞圓縮，細胞核變形，染色質濃縮且邊位，細胞質空泡狀；細胞死亡率隨處理時間的增加而增加；細胞內鈣離子濃度隨處理時間延長而遞增；線粒體膜電位差在低溫處理早期急速上升，隨后一直下降；細胞內超氧陰離子（ o _ 2 ~ - ）在低溫處理前期出現高峰，接著呈下降趨勢；細胞內sod活性在低溫處理前期減弱，接著上升，然後持續下降。2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。Section four : effects of copper and cadmium on ultrastructure of myocardial cell in sinopotamon yangtsekiense the effect of copper and cadmium on ultrastructure of myocardial cells of sinopotamon yangtsekiense was studied by us
銅、鎘聯合作用30d后，細胞核形態進一步改變，內外膜分離程度加大，異染色質疑集加重。線粒體膜破裂，內容物外流，嵴斷裂消失。Plasmids are exta - chromosomal doublestranded, circular dna molecules found in prokaryotic and eukaryotic cells
質粒是在原核和真核細胞中發現的染色體外的雙鏈環狀dna分子。Another 561 bp fragment of the orf of p22 gene was amplified by pcr, and digested by pst1 and xba 1. the amplified fragment was cloned into pbudcfal to construct the recombinant plasmid pbudce4. 1 / p22. then transfected it into the murine macrophages by using lipofectamine, the expression of p22 - mrna in the transfected macrophages was identified by rt - pcr
同時擴增p22編碼基因orf的全長561bp片段，經pst 、 xba雙酶切后，定向克隆于質粒pbudce4 . 1 ，構建真核重組重組表達質粒pbudce4 . 1 / p22 ，通過脂質體介導轉染入小鼠巨噬細胞raw264 . 7 ，以rt - pcr方法鑒定目的基因在巨噬細胞中的表達。Most ones were these disorganized partly, mainly, till all, mitochondria fused by itself, circled, mitochondria membrane broke and disintegrated
處理20d后，細胞核與線粒體受損情況加重，主要表現在，細胞核膜間距加大，異染色質疑聚，常染色質電子密度降低。This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。Sds - page and westen - blotting validated the expression of pp24. the expressed specific band was excised from the gel and injected into mice once two weeks for 5 times, then we collected the antiserum from the mice and used it for ifa with chicken embryonic fibroblasts ( cef ) infected by mdv mdll, cvi988 and ga strains respectively. the positive straining was found in the mdv plaques, which shows the in vitro expressed protein of pp24 has some epitopes of mdv
將型mdvmd11株的pp38基因和pp24基因的完整orf分別克隆入真核表達載體pcdna3 . 1 / zeo （ + ）中，重組質粒pcdna3 . 1 - pp38和pcdna3 . 1 - pp24在脂質體作用下分別轉染cef ，在轉染后24 、 48 、 72小時，用單抗h19和pgex - pp24原核表達產物制備的抗血清分別對pcdna3 . 1 - pp38和pcdna3 . 1 - pp24轉染的cef進行ifa檢測，結果檢測到了pp38和pp24在cef中的表達，該試驗也使pp24基因在原核和真核系統中的表達得到了相互驗證。分享友人