核殼體蛋白 的英文怎麼說
中文拼音 [hékétǐdànbái]
核殼體蛋白
英文
nucleocapsid protein-
Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法,並試圖在預防上提供有效的免疫制劑,本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化;並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。It was thought earlier that multiple copies of no more than a few different proteins make up the protein "coat" of the ribosome.
人們以前認為組成核蛋白體蛋白質「外殼」的只不過是幾個不同蛋白質的重復拷貝。The 21kda protein was specifically located in the nucleocapsid of occlusion - derived and not in that of budded virions
進一步的研究顯示, ha122蛋白特異的存在於多角體病毒核衣殼中。Meanwhile, these six coat protein genes were sequenced and compared with other homologous sequences in genbank. the strain designation of the six isolates of tumv was finally determined. the results are as the following : 1. six isolates of turnip mosaic virus named tumv - sd1, tumv - sd2, tumv - sd3, tumv - sd4, tumv - sd5 and tumv - sd6 were respectively acquired from infected chinese cabbages and radishes in 3 cities ( taian, yantai and zaozhuang ) of shandong province
利用rt - pcr方法克隆了tumv山東分離物的外殼蛋白( cp )基因,測定了它們的核苷酸序列、並將其與已報道的序列進行同源性比較和分析,最終確定了其歸屬地位,具體研究結果如下: 1 .從山東省3地市感病的白菜和蘿卜上分離到蕪菁花葉病毒的6個分離物,分別命名為tumv - sd1 、 tumv - sd2 、 tumv - sd3 、 tumv - sd4 、 tumv - sd5和tumv - sd6 。In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera, the ecori - n fragment located at 54. 8 - 59. 3 kbp of the viral genome was sequenced. the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda
對棉鈴蟲單核衣殼核多角體病毒( helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus , hasnpv )基因組中ecori ? n片段進行序列分析,獲得了完整的解螺旋酶基因( hel ) ,其開放閱讀框大小為3762bp ,編碼一個分子量為146kda的蛋白質。The surface - modified magnetic nanoparticles have been widely used in biological and biomedical areas such as immobilization of biomolecules ; separation of metal ions, cells, proteins, dna ; immunoassay, biochemical analysis and magnetic resonance imaging ( mri ) probes
其中的殼結構既具有生物適應性,又具有可鍵合生物分子如細胞、蛋白質、酶、抗體和核酸的活性基團,而核具有磁性特性。At the same time, vp4 gene was mutated in a certain point by pcr. the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly. a. tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l. guad according to the leaf disc procedure
為便於基因操作,對外殼抗原蛋白vp4基因進行適當的修飾和改造:通過引物設計,利用pcr反應,在基因的起始編碼前引入有助於真核生物表達的kozak序列和限制性內切酶位點;使用套疊pcr對vp4基因進行定點突變,以便於將改造后的基因插入pbi121構建植物表達載體,通過直接轉化法,把pbi121 vp4轉入農桿菌eha105 ,構建了農桿菌工程菌。分享友人