核蛋白微粒 的英文怎麼說
中文拼音 [hédànbáiwéilì]
核蛋白微粒
英文
ribonucleoprotein particle-
Histologically, diffuse distribution of eosinopholic cardiocytes with pyknosis was noted. immunohistochemical staining for myoglobin discovered wide and diffuse loss of myoglobin throughout the ventricular myocardium. ultrastructurally, the myocardial cell with myoglobin loss were characterized by the presence of swollen mitochondria and packed cellular constituents
組織學檢查注意到彌散性分佈伴有核碎裂的嗜酸性變性，肌紅蛋白組化染色發現整個心室肌廣泛性脫失，超微結構研究表明脫失肌紅蛋白的心肌細胞存在線粒體腫脹和細胞內成分的堆積。Global or filament actin was visualized in cytoplasm and nucleus of tobacco pollen mother cells, under immunoelectron microscope
免疫電鏡觀察發現，花粉母細胞中的細胞質及核中存在線狀和粒狀的肌動蛋白微絲。This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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