核酸肽 的英文怎麼說

中文拼音 [hésuāntài]
核酸肽 英文
peptide nucleic acid
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 肽 : 名詞[化學] （有機化合物） peptide
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  1. Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin

    這些基因中有和肝再生有直接關系的如：胎球蛋白、組織蛋白酶；和肝臟功能密切相關的如：胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶；與物質能量代謝有關的如： atp合成酶、核糖體蛋白；以及與急相反應有關的如：觸珠蛋白、轉鐵蛋白。
  2. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  3. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  4. Background : ghrelin is a 28 - amino acid endogenous peptide recently identified in the secretory granules of x / a - like cells in the rat stomach, which can act on the growth hormone secretagogue receptor to accelerate the secretion of growth hormone. it was identified that ghrelin - immunoreactive cell and its receptor localize in areas of brain, such as hypothalamus ( pvn, arc ), hippocampus, adenohypophysis and so on. motilin is a 22 - amino acid peptide secreted from the upper part of the small intestine, which regulates the interdigestive motility of gastric

    背景資料： ghrelin是新發現的由28個氨基酸組成的內源性腦腸肽，由胃部泌酸腺x / a樣內分泌細胞分泌， ghrelin免疫活性神經元及其受體在中樞神經系統中如下丘腦室旁核（ paraventricularneucleus ， pvn ） 、弓狀核（ arcuatenucleus ， arc ） 、海馬區及腺垂體等均有不同濃度的分佈，可與生長素促泌物受體（ growthhormonesecretagoguereceptor ， ghsr ）結合促進生長素（ growthhormone ， gh ）分泌。
  5. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  6. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  7. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  8. Hovever, the gene encoding the mature papain peptide was amplified using pcr from genomic dna extracted mid - development carica papaya fruit. about 98. 8 % of cdna nucleotide sequence reported in literature were homologous to the responding sequences of our study. there are three introns in the gene, in which the content of a and t is 86. 0 %, 79. 5 % and 90. 6 % respectively

    同時本研究以木瓜基因組dna為模板，通過pcr反應獲得了編碼木瓜蛋白酶成熟多肽部分的核苷酸序列，序列分析表明該基因含有三個內含子，其長度分別為157bp 、 266bp 、 88bp ， at含量分別為86 . 0 ， 79 . 5 、 90 . 6 。
  9. The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification

    本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。
  10. Moreover, human ghrelin receptor exhibits a remarkable 50 % overall identity with human motilin receptor, therefore it was named as " raotilin - related peptide ". anatomical evidence showed that there are ascending and descending neuronal projections between pvn, bma and lower brain stem ( nts, dmx ), which participate in the regulation of gastric acid secretion and gastrointestinal motility. moreover, pvn is the center of appetite regulatory web

    Pvn與低位腦干（延髓迷走復合體）及邊緣系統之間有密切的纖維聯系，且含有多種神經肽，參與胃酸分泌，胃腸活動等多種生理機能的調節，是影響消化道功能與攝食行為的重要中樞部分， pvn內胃動素可促進胃排空和攝食，而新發現的ghrelin /胃動素相關肽是否參與此核區對胃運動的調節機制及其可能的神經通路尚不明了。
  11. Appliance of peptide nucleic acid in molecular biological technology and disease therapy a review

    肽核酸在分子生物學技術和疾病治療方面的應用綜述
  12. A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs

    本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽（ transporter ， trs ）基因核苷酸序列，藉助oligo4 . 1設計了一對寡核苷酸引物，以引物3末端的短互補序列退火形成小段雙鏈，從而互為模板和引物，通過引導合成長達90bp的gnrh trs序列gnrh trs 。
  13. In this study, it has been put forward that taking reactive nanometer magnetic fe304 particles as magnetic nucleus, and the copolymer of styrene ( st ) ? crylic acid ( aa ) as macromolecular shell, we could synthesize, magnetic polymer composite microspheres containing carboxyl groups on their surface, then microspheres are activated by thionylchloride, the surface of such magnetic composite microspheres thus produced had reactive acid chloride groups which then react with the free amino groups of the free soluble enzymes to give peptide bonds ( ? o ? h ?,

    本研究首次提出了以納米級磁性fe _ 3o _ 4粒子為核心，苯乙烯（ st ） ?丙烯酸（ aa ）共聚物為高分子殼層，合成了表面帶羧基的磁性高分子復合微球，然後將這種微球用二氯亞碸進行活化處理，在其表面形成了反應性酰氯基團，該基團可以與游離酶的氨基形成肽鍵，從而將游離酶固定化。
  14. After sequencing of 84 cdna clones and removing redundant cdnas, we obtained 36 cold - regulated unique cdna clones. 12 cdna clones were expected to be novel genes, because no sequence homology with any known sequences was found in genbank databases

    全長基因ej175共有603bp ，對其可讀閱讀框架進行分析，從57 - 515位核苷酸的一段序列，包含了起始密碼子和終止密碼子，編碼152個氨基酸的多肽。
  15. The cdna of subunit b was 1470 nucleotides long coding for 489 amino acids with a conservative atp binding site " 324 - sgsit - 328 " and a predicted molecular mass of 54. 29 kda. amino acid sequence alignment analysis suggested that the v - h + - atpase b subunit of s. salsa had high homology with other reported v - h + - atpase subunit b. the h + - atpase subunit h from s. salsa was a hydrophilic protein with 465 amino acids and a predicted molecular mass of 52. 8 kda, which was encoded by a cdna with 1398 nucleotides in orf. blast analysis indicated that the h + - atpase subunit h from s. salsa had a high amino acid sequence identity with those coming from plants, but had relatively low sequence identity with those coming from other species

    鹽地堿蓬液泡膜h ~ + - atpaseb亞基是由1470個核苷酸編碼的長達489個氨基酸的多肽，分子量約54 . 29kda ，存在保守的atp結合序列「 324 - sgsit - 328 」 ，與其他物種來源的v - h ~ + - atpaseb亞基具有較高的氨基酸序列相似性； h亞基為親水多肽，開放閱讀框長達1398 - bp ，編碼465個氨基酸殘基，分子量約52 . 8kda ，與植物來源的液泡膜h ~ + - atpaseh亞基具有較高的氨基酸序列相似性，而與其他物種來源的v - h ~ + - atpaseh亞基同源性較低； c亞基開放閱讀框為495 - bp ，編碼164個氨基酸殘基，分子量約16 . 6kda ，為一跨膜多肽，存在四個可能的跨膜區。
  16. Due to high homology of nucleotide between ba - dfe and subtilisin bpn, primers were designed and synthesized. the intact ba - dfe gene was amplified by pcr and cloned. the sequence analysis indicated that the ba - dfe gene has an open reading frame with 1146bp, which encodes 382 amino acid residues containing signal peptide, pro - peptide and mature peptide

    序列分析顯示，該片段的核昔酸序列中含有1146hp的開放閱讀框，可編碼382個氨基酸殘基的ba dfe前體蛋白，包括30個氨基酸殘基組成的信號肽、 77個氨基酸組成的前導肽和275個氨基酸殘基組成的成熟肽。
  17. The open reading frame consists of a series of tri - nucleotide codons which are translated into specific amino acids in the lengthening polypeptide

    由一系列三核苷酸密碼子組成，多肽延伸時翻譯成特定氨基酸。
  18. Research to date suggests that sense and antisense peptides coded for by mutually complementary nucleic acid sequences are capable of interacting specifically, thereby suggesting the existence of a second, two - dimensional genetic code ( i. e., proteomic code )

    研究表明由互補的核酸序列編碼的有義肽和反義肽之間存在特殊的相互作用，由此就提出了有第二套二維的遺傳密碼即蛋白密碼的存在。
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