核酸與基因組 的英文怎麼說

中文拼音 [hésuānyǔjīyīnzǔ]
核酸與基因組 英文
nucleic acid and genomics
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  1. Based on recent molecular phylogenetic analyses using nucleotide sequences of the encoding the large subunit of ribulose 1, 5 - bisphyosphate carboxylase / oxygenase ( rbcl ), hypodematium should be not included in the athyriaceae, it has closely related to dryopteridaceae. on the other hand, athyriaceae, thelypteridaceae, blechnaceae, onocleaceae and woodisaceae form a large clade, so it may explain that tryon & tryon ( 1982 ) and kramer & kato ( 1990 ) putting it forward as dryopteriaceae s. 1

    運用cpdna基因組編碼的磷酸核酮糖羧化酶大亞基（ rbcl ）的基因序列測定而構建的系統樹，顯示蹄蓋蕨科、金星蕨科、烏毛蕨科以及其他科構成一條與鱗毛蕨科平行的分支，因此可以說明kramer & kato （ 1990 ）把蹄蓋蕨科放入廣義的鱗毛蕨科是不合理的。
  2. Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin

    這些基因中有和肝再生有直接關系的如：胎球蛋白、組織蛋白酶；和肝臟功能密切相關的如：胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶；與物質能量代謝有關的如： atp合成酶、核糖體蛋白；以及與急相反應有關的如：觸珠蛋白、轉鐵蛋白。
  3. Tsarg2 with 1 233 bp length was composed of 6 exons and spaned about 115 kb of genomic dna, the putative protein encoded by this gene was 305 amino acid with a theoretical mass of 34 751 and with no significant homology with any known protein in databases. a kind of nucleoprotein was the most impossible

    Tsarg2基因的cdna全長為1233bp ，包含6個外顯子，基因組跨越115kb ，編碼由305個氨基酸組成的、分子量為34751的蛋白質，與已知蛋白質無明顯同源性，其最大可能是一種核蛋白。
  4. When using the nuclear acid sequence and the predicted amino acid sequence of scghr to blast the fugu genome, we found that there were two contigs which shared the most similarity with scghr. therefore we realized there maybe were two ghrs encoded by different genes in teleosts. then, by means of nuclear acid sequence joining, orf of fghr1 and fghr2 were predicted from fugu genomic sequences using cohoghr isf1, cohoghr isf2, chghr and other related ghr amino acid sequence available at ncbi database

    當利用南方鯰ghr的核苷酸、氨基酸序列blast東方?的基因組時，發現有兩個contig與其氨基酸序列非常相似，因此意識到魚類可能存在兩種不同基因編碼的ghr 。隨後主要利用已經克隆得到的cohoghrisf1 、 cohoghrisf2 、 chghr以及其他魚類ghr核苷酸和氨基酸序列blast東方純的基因組，採用序列拼接的方法從東方?基因組中分離出兩種不同基因編碼的fghr1和fghr2 （本研究把和傳統ghr相似的叫做ghr1 ，把另外一種叫做ghr2 ） 。
  5. Determination and analysis of complete nucleotide sequence of swine vesicular disease virus

    豬水泡病病毒全基因組核苷酸序列的測定與分析
  6. Rt - pcr was used to amplify the cdna of the genome. these cdna fragments were cloned into the plasmid pucm - t. the result indicated that the seguence of the genome was obtained. the genome of aev - nh937 composed of 7055 nucleotides, potentially encodes a polyprotein of 2134 amino acids. the genome of aev - nh937 has 94. 3 % nucleotides identity with the calnek vaccine strain of aev

    把它們分段克隆在pucm - t載體上，經序列分析，獲得了aev - nh937毒株的基因組全序列及推導的氨基酸序列，基因組全長為7055個核苷酸，與calnek疫苗株具有94 . 3的同源性，編碼一個含2134個氨基酸的多聚蛋白。
  7. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  8. ( 3 ) the data support that roe deer ( capreolus capreolus ) moved into hydropotinae ( presently in which there is only one species : hydropotes intermix ) and stemed its own independent genus ; ( 4 ) the results are object to the usual standpoint that dusky musk deer and alpine musk deer are two different species ; ( 5 ) it is more reasonable that moschidae ( musk deer ) is placed at the level of super family ; ( 6 ) the number of transition within 12s rrna is within the range of 2 to 20 times bigger than that of transversion ; ( 7 ) as to nucleotide composition, adenine ( a ) is much more abundant than any of other bases ( averagely 36. 88 % ) and guanine ( g ) is the least ( averagely 17. 65 % ) in the complete sequence, which is similar with the loop region

    （ 4 ）黑麝與馬麝的12srrna基因序列完全相同， 12srrna基因數據不支持黑麝與馬麝為兩個種。 （ 5 ）本實驗結果支持麝作為上科的分類階元存在。 （ 6 ） 12srrna基因的堿基代換中轉換數遠遠大於顛換數（ 2 - 20倍） （ 7 ） 12srrna基因二級結構中，全序列核苷酸的堿基組成腺嘌呤（ a ）最多（平均為36 . 88 ） ，鳥嘌呤最低（平均17 . 65 ） ，與環區的堿基組成相類似。
  9. The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53. 484 kda and an isoelectric point of 6. 75. at the primary sequence level, it shared high homology with clr, so was named clr - like serine protease, clsp. the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 )

    該蛋白在核酸和氨基酸水平上與人補體組分cl :表現高度同源，故將其命名為補體cl :樣絲氨酸蛋白酶（旦lr一like旦erineprotease ， clsp ） ，該基因已經在ge心ank登錄（登錄號為af17s985 ） 。
  10. All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains, and there were many difference between velogenic strains and lentogenic strains. so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication, pathogenicity of ndv and the interaction between ndv and host cells, and the infectious cdna of velogenic strains ( eg. f48e9 ) was required to explain the relationships between structure and function

    本研究成功地獲得了ndvf48e9 t因組的核昔酸序列，並構建了表達ndvf48e9基因組cdna的低拷貝表達載體休f48e9 ，為構建新城疫病毒強毒株f48e9株的感染性cdna奠定了物質基礎，進一步研究ndv的生物學特性、結構與功能的關系；進一步探討影響ndv毒力的因素、以及研製新型疫苗載體提供了可靠保證。
  11. The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous, but lowly homologous with other reference strains. the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains, and is identical with the facts in the field cases. all the guangxi isolates are classified into genotype vii of apmv - 1, the same genotype dominated in china and other areas in recent years

    結果發現，廣西分離株之間在信號肚的核旮酸和氨基酸同源性很高，而與其它參考株差異較大；廣西分離株在裂解位點的氨基酸組成和排列均符合強毒株的特徵，並與毒株在臨床上的致病情況相符；根據apmvlf基因第47位第420位核苦酸序列所繪制的系譜樹吵ylogenetictree ）來看，廠西雞和鵝分離株都歸屬于基因型vll 。
  12. Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained

    再次，根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物，與atp結合蛋白的上游簡並引物組合，經pcr擴增獲得2 . 1kb的條帶，測序后通過blast比較，結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。
  13. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  14. Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected

    Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。
  15. An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene, fnr gene ) in vero cells. interestingly, we found that the nontargeted mutation frequency induced by mnng was increased significantly, implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation

    利用反義核酸技術構建含反向插入9號片段的真核細胞表達重組體並轉染細胞，以獲得反義rna阻斷vero細胞中相應基因的表達，發現mn 』 ng誘發的非定標性突變頻率顯著增高，提示被阻斷的相關基因的表達產物可能參與抑制非定標性突變的發生。
  16. The gene was cloned into puc19 vector and identified with pcr and eco ri + hind iii digestion. then, one of the positive recombinant clone was sequenced and analysed. the result showed that its sequence was 35 % and 32 % identical to equine ifn - and human ifn - respectively, but it only shared 15 % homology with chicken type i ifn

    序列分析表明，該基因閱讀開放框架由492個核苷酸組成，與馬和人ifn -基因序列的同源性分別為35和32 ，與雞i型干擾素基因的同源性僅為15 ，與國外報道的雞ifn -基因序列完全一致。
  17. However, we found a reigon of 30 amino acids in l protein ( aa 1287 - 1318 of the respective l protein ) of f48e9 which is different from the la sota, zj1, b1 and beaudette - c and having a higher homology with v4 and clone30 strain

    東北農業大學農學碩士學位論文一對六株ndv分離株全長基因組核音酸同源性進行比較發現， f48e9株與lasota 、 clone30 、 zji 、 v4和bi的核昔酸同源性分別為： 87 3 、 87 7 、 85
  18. Using random primed gene walking pcr ( rpgw - pcr ), ctsox2 gene was cloned and sequenced from the digested genomic dna of trionyx sinensis. compared with the mouse and chicken sox2, ctsox2 shared 86 % and 91 % nucleotide homology respectively, and 93 % and 97 % amino acid identity respectively

    天i消化過的中華鱉（ trio矛移優sinensis ）基因組dna中克隆了c眨治xz基因（ 72obp ） ，它與鼠、鳥的及戲2基因分別有85 %和91 %的核昔酸同源性， 93 %和97 %的氨基酸同源性。
  19. And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8

    試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。
  20. In this research, the gpv hl isolate was propagated with 13 day ' s duck embryos. a pair of primers gflgr used to arnplify vp3 gene was designed using oligo4. i software according to the whole nucleotide sequence of gpv b isolate published by zadori. the major structural protein vp3 gene was amplified from the dna of gpv hl isoiate by polymerase chain reaction ( pcr ), and then cloned into pmdl8 - t vecter

    根據zadori等發表的gpvb株全基因核苷酸序列，藉助oligo4 . 1軟體設計了1對用以擴增主要結構蛋白vp3基因的引物gf / gr ，通過pcr技術，從病毒基因組dna中擴增出病毒主要結構蛋白vp3完整基因片段，經酶切鑒定后直接與pmd18 - t質粒載體連接。
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