根瘤菌菌株 的英文怎麼說

中文拼音 [gēnliújūnjūnzhū]
根瘤菌菌株 英文
rhizobium strains
  • 根 : Ⅰ名詞1 （植物的營養器官） root （of a plant） 2 （比喻子孫後代） descendants; posterity 3 [數學] ...
  • 瘤 : 名詞（瘤子） tumour
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 株 : Ⅰ名詞1. （露在地面上樹木的根和莖） root and stem of a tree above the ground 2. （植株） individual plant; plant Ⅱ量詞（棵）
  • 菌株 : bacterial strain
  1. Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant

    本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。
  2. Compared to 042bm, the noea deletion mutant 042bma - km showed different degree of increase in nodule number, weight of nodule nodule and plant top dry weight on cultivars of putong zihua, baoding, ningxia, baifa and aohan, but decrease on milu. however, this mutant has no significant change in ability to nodulate cultivars of huanghou and zahua. hence, noea is involved in cultivar - specific nodulation

    與苜蓿中華根瘤菌042bm相比，敲除noea的突變株042bma - km在普通紫花、保定、寧夏、百發和傲漢苜蓿品種上的結瘤數、根瘤鮮重和植株地上部分的干重都有不同程度的增加，而在秘魯苜蓿品種上的結瘤數和植株地上部分的干重明顯下降，在皇后和美國雜花苜蓿品種上則沒有明顯的變化。
  3. Results of host - plant range test shown that all slow - growing isolates could effectively form nodules on arachis hypogaea and phaseolns vulgaris. they could not nodulate on visia sativa, and part of them could nodulate glycin soja and glycin max

    宿主范圍研究表明供試花根瘤菌均能在花生上進行有效的結瘤並固氮；部分菌株能夠在野大豆和栽培大豆矮腳早上結瘤；所有供試菌株均不能在野豌豆上結瘤，但均能能在菜豆上結瘤且單株瘤數較多。
  4. This paper concerns studies on rhizobia in symbiosis with important legume soybean and peanut crops in china, following : 1 ) summarized bio - diversity of rhizobia, including indigeneous population, isolation frequency, strain type, distribution, and strain - host promiscuity and compatibility from large and small samples. 2 ) reviewed symbiont improvement in both sides of rhizobia and hosts, involving selection of effective rhizobial strains, evoluation and utilization of the host germplasm for symbiotic properties, and selection of the both sides for special characters. 3 ) discussed the subject on the rich symbiont germplasm resource in china and on further research being necessary to exploitation and utilization of the benificial germplasm

    針對我國主要豆科作物大豆和花生的研究成果，並結合本身研究工作內容， （ 1 ）總結了與宿主共生的根瘤菌生物學多樣性，包括大、小樣本的土著根瘤菌群體數量、分離頻率、類型與分佈及其菌株宿主共生混交性與親和性； （ 2 ）評述了根瘤菌宿主植物共生體雙邊固氮改良，包括優良菌株的選育、宿主品種資源共生特性的評價與利用及特異性狀的選擇； （ 3 ）討論了我國這一類共生體資源的豐富性以及有必要進一步加大力度研究有益資源的開發和利用的問題。
  5. Abstract : this paper concerns studies on rhizobia in symbiosis with important legume soybean and peanut crops in china, following : 1 ) summarized bio - diversity of rhizobia, including indigeneous population, isolation frequency, strain type, distribution, and strain - host promiscuity and compatibility from large and small samples. 2 ) reviewed symbiont improvement in both sides of rhizobia and hosts, involving selection of effective rhizobial strains, evoluation and utilization of the host germplasm for symbiotic properties, and selection of the both sides for special characters. 3 ) discussed the subject on the rich symbiont germplasm resource in china and on further research being necessary to exploitation and utilization of the benificial germplasm

    文摘:針對我國主要豆科作物大豆和花生的研究成果，並結合本身研究工作內容， （ 1 ）總結了與宿主共生的根瘤菌生物學多樣性，包括大、小樣本的土著根瘤菌群體數量、分離頻率、類型與分佈及其菌株宿主共生混交性與親和性； （ 2 ）評述了根瘤菌宿主植物共生體雙邊固氮改良，包括優良菌株的選育、宿主品種資源共生特性的評價與利用及特異性狀的選擇； （ 3 ）討論了我國這一類共生體資源的豐富性以及有必要進一步加大力度研究有益資源的開發和利用的問題。
  6. Identification of functional bacteria showed predominant ammonifiers were shewanella, variovorax, chryseobacterium, bacillus or aeromonas ; among 4 selected nitrogen fixers, one ( azorhizobium caulinodans ) belonged to. a - proteobacteria, the other three ( serratia marcescens, klebsiella pneumoniae and citrobacter freundii ) were enterobacteriace, which belongs to - proteobacteria ; 2 nitrate reducers were aeromonas sp. and citrobacter sp.,

    對各功能菌群中的優勢菌的鑒定表明，優勢的氨化細菌為希瓦氏菌屬，產堿菌屬，黃桿菌屬，芽孢桿菌屬或氣單胞菌屬；分離到的4個優勢固氮細菌菌株中，一株為基瘤固氮根瘤菌，屬于-變形菌亞門，而另外3株都屬于腸桿菌科，歸于-變形菌亞門。
  7. At relatively higher similarities, strains tested from different area were further clustered which reflected the effect of geographical factor on rhizobia genetic diversity

    在較高的相似性水平上來自不同地域的菌株可進一步細分為不同的小群，並表明了地理因素對根瘤菌遺傳多樣性的影響。
  8. The suitable concentration of hyg to screen for resistant shoots was 50 mg / l. the kind of antibiotics to inhibit the growth of agrobacterium eha105 was cef, of which concentration was 250 mg / l

    以洋桔梗葉盤為外植體，通過根瘤農桿菌菌株eha105介導，用構建於質粒pcambia1301的npr1基因轉化洋桔梗。
  9. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。
  10. Genetic group 3 included 2 new isolated strains and 3 representative strains ( usda6t, b15, usda110 ) of b. japonicum

    Elkanii的參比菌株和9株待測葛藤根瘤菌組成，遺傳群3由3株b
  11. Phenon 5 included 4 new isolated strains and 3 representative strains ( usda6t, b15, usda110 ) of b. japonicum., the new isolated strains in this phenon probably are b. japonicum

    Japonicum的參比菌株聚在一起，群內相似性為83 ，初步判定這群葛藤根瘤菌為b japonicum 。
  12. In numerical taxonomy, phenon 1 included 9 new isolated strains and 1 representative strain ( usda761 ) of b. elkanii, the new isolated strains in this phenon probably are b. elkanii

    Elkanii的模式菌株（ usda76 ~ t ）聚在一起，群內相似性為80 ，初步判定群1供試葛藤根瘤菌為且belkanii 。
  13. Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy, including numerical taxonomy, rep - pcr fingerprinting, 16s rdna pcr - rflp. the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp. showed great diversity. ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains, the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid. the other strains were slow - growing strains, their single colony size was less than 1 mm after 7 days incubated on yma medium at 28. they can produce alkali

    本研究以從我國四川、河南、安徽和湖南等地分離的32株葛藤根瘤菌為研究對象，以20株已知種的根瘤菌為參比菌株，採用數值分類、 rep - pcr指紋分析、 16srdnapcr - rflp指紋分析等現代根瘤菌分類技術，初步研究了葛藤根瘤菌的生物多樣性和分類地位，結果表明：葛藤根瘤菌在生長速率上表現出多樣性，菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生長較快， yma培養基上28培養2 - 3天後，單個菌落直徑大於1mm ，具有產酸能力，是快生型葛藤根瘤菌；其餘待測葛藤根瘤菌生長較慢， yma培養基上28培養7天後，單個菌落直徑小於1mm ，具有產堿能力，是慢生型葛藤根瘤菌。
  14. ( 19 98 ) got after the nif - rflp analysis of coriaria strain 030. it is also accordant with the result that nick et al. ( 1992 ) got with the anaylsis of partial sequence of some coriaria isolates

    這一結果與胡傳炯等（ 1998 ）對馬桑菌株cs030進行nif ? rflp分析所得的結論以及nick等（ 1992 ）在檢測馬桑根瘤內生菌的部分序列后所得的結論都是相一致的。
  15. Results of g + c mol % test shown that all slow - growing isolates were belong to the same species. more than 70 % dna - dna homologies were determined among 4 representative strains usda6, and usda110 ( type strains of b. japonicum ). low dna homologies were detected with usda76 ( type strain of b. elkanii )

    中l習花生根瘤菌遺傳多樣性和系統發育研究g + cmol %和dna一dna同源性分析結果表明，供試花生根瘤菌代表菌株的tm和g + cmol %均小於種內變異l隔度，表明供試花生根瘤菌均屬j二一個種。
  16. The result of rflp analysis of pcr - amplified 16s rdna revealed that the rhizobia isolated from the root nodules of pueraria spp. had diversity at the level of species. the slow - growing rhizobia were divided into 7 groups on the similarity level of 90 %. group 1 included 4 new isolated strains and 2 representative strains of b. japonicum. group 2 included 1 type strain of b. elkanii and 3 new isolated strains

    16srdnapcr rflp分析結果也表明葛藤根瘤菌具有種水平多樣性，在90的相似性水平上，聚類圖把慢生根瘤菌分成7群，群1與bjponicum的模式菌株聚在一起，群二與belhanii的模式菌株聚在一起，而其它群是獨立的系統發育分支。
  17. The sds - page electropheresis of whole - cell proteins was applied in classification of 71 strains isolated from astragalus spp. it was showed that the technique is a simple and rapid method in classification of rhizobia. the similarity of strains in the same group is 78 %, and dna homology is above 70 %

    採用sds page技術對71株黃芪根瘤菌進行了全細胞蛋白的聚類分析.結果表明，這是進行根瘤菌分類時一種簡便快速的分群方法，分群的菌株相似性水平為78 ，群內菌株的dna同源性70
  18. Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073. marker exchange was achieved by selection on yma containing sm, km, gm, spc et al four kinds of antibiotics. mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively

    隨后，利用突變質粒pgxn217誘變慢生型大豆根瘤菌菌株gx201 ，獲得了gx201的突變體菌株gx217 ；將pgxn201的3 . 4kbecori片段與plarf3連接獲得亞克隆pgxn201cl ，然後將pgxn201cl導入突變體菌株gx217 ，構建了gx217的功能互補菌株rgx217 。
  19. Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1

    通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。
  20. Combining the analysis of the conservative region of 16s rrna gene of prokaryote, such as e. coli, rhizobia and several frankia strains, we designed several sets of primers to amplify the 16s rrna gene of the frankia strains tested. through tentative experiments with these primers, we screened out primers uf / ur and ec27f / frl717r

    通過比較已發表的原核生物，如大腸桿菌、根瘤菌和弗蘭克氏菌的16srrna基因全序列的保守區，設計了8對引物並篩選出可適用於擴增13株供試菌株16srrna基因接近全長序列的引物： uf ur和ec27f fr1717r （產物大小約1500bp ） 。
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