桑椹胚 的英文怎麼說
中文拼音 [sāngshènpēi]
桑椹胚
英文
morula-
The fresh mouse morulae were bisected following decompaction ( experiment 3 ) and other morulae without decompaction ( experiment 4 ) also splitted
新鮮桑椹胚經去緻密化處理後分割,其分割成功率、半胚培養后的囊胚發育率都顯著高於未處理組。Two stage embryos were observed their effect on production of chimera. the result shows that there is a significant different ( 0. 01 < p < 0. 05 ) between blastocysts ( including early blastocysts and morulae ) and expanded blastocysts
結果表明,囊胚、早囊及桑椹胚同擴張囊胚兩種注射胚胎製作嵌合體所產生的結果差異顯著( 0 . 01 p 0 . 05 ) 。Experiment i study on the embryonic development by injecting different developmental stage embryos. this experiment collected 109 embryos to observe their developmental ability by es cells microinjection into 8 - 16 cell stage morula or blastocyst
本試驗先後將109枚胚胎用於此項研究,通過將小鼠es細胞注入8 - 16細胞桑椹胚和囊胚,觀察兩個不同時期注射胚胎的發育能力。Ppar5 expression in the uterus of ovariectomized mice was significantly stimulated by estrogen or a combination of estrogen and progesterone. ppar8 mrna was detected in oocytes and preimplantation embryos at the stages of zygote, 2 - cell, 4 - cell, 8 - cell, morula and blastocyst
在小鼠卵母細胞、受精卵、 2細胞、桑椹胚和胚泡中,可檢測到ppar表達,但在4細胞和8細胞中沒有檢測到ppar基因的表達。The demi - embryos derived from vitrified morulae in ops ( experiment 7 ) and fresh morulae ( experiment 8 ) after cultivation for 24h were transferred to day 2. 5 to day 3 pseudo - pregnant female mice. in experiment 7, 15 fetuses were obtained from 5 of 11 recipients which had received 191 demi - embryos
對ops法冷凍桑椹胚和新鮮桑椹胚分割后移植,將各自分割所獲得的半胚191枚和182枚,分別移植給11隻和10隻假妊娠2 . 5 3d的受體母鼠。Recovery of microtubule organizing centers in enucleated oocytes and condensation of chromosome and organization of spindle in reconstructed embryos were analysed. a non - invasive method of enucleation mouse oocytes has been developed. firstly, the progression of mouse oocytes in meitosis i was ascertained by immunofluoresence technique
另外,本試驗通過在m16基礎上添加eaa 、 non - eaa和葡萄糖顯著提高了胚胎的桑椹率、囊胚率和孵化率;單獨添加葡萄糖的培養液,其桑椹率顯著升高,但囊胚率和孵化率的升高並不顯著,所以胚胎培養液中氨基酸和葡萄糖能協同促進胚胎發育。2 ) the rat - mouse reconstructed embryos activated were been cultured in the maturation mediums of tcm199, ml6, czb and czbm ( czb modified ) for 48h
直接注射法和融合法構建的重組胚桑椹胚發育率分別可達9 . 84 、 9 . 48 。There was no significant difference between the fusion rates of reconstructed embryos. the cleavage rate of embryos reconstructed with c57bl / 6j oocytes was significant high than that of kunming albino, but the former only developed to 4 - cell stage ; the later could develop to blastocyts. it is implied that the oocytes of inbred mouse cannot support the long - term development of kunming es cell
兩種重構胚的融合率之間沒有顯著差異( p 0 . 05 ) , c57bl 6j重構胚的卵裂率( 60 . 5 )明顯高於昆明白小鼠( 33 . 7 ) ,但是前者只發育到4細胞期,而後者可以發育到桑椹期和囊胚期( 2 . 3 ) 。Mouse blastocysts and morulas were cultured in rat heart conditioned medium ( rh - cm ). as far as the following indexes attaching ratio, icm growth ratio and es clone formation ratio are concerned, the choice of blastocysts is much better than morulas ( p < 0. 05 ), therefore mouse blastocysts were more suitable than morulas to get es cells
源於icr小鼠icm的es細胞分離克隆研究試驗以rh - cm為培養基培養處于不同發育階段的小鼠胚胎,發現由囊胚分離的es細胞傳代后es集落的出現率顯著高於桑椹胚( p 0 . 05 ) ,故囊胚更適合作為es細胞分離克隆的材料。164 microinjected embryos and 121 noninjected embryos were transferred to 14 recipient gilts. of this, 159 microinjected and noninjected embryos ( blastocyst, early blastocyst and morula ) was transferred to 6 recipient gilts, three pigs are pregnant ( one gilt delivered 8 piglets, two are skin pigment chimera ), and pregnancy and chimera rate is 17 % ( l / 6 ). 126 microinjected and nonmicroinjected expanded blastocysts oestrused on time, pregnancy and chimera rate is 0 ( 0 / 5 )
其中, 159枚囊胚、早囊及桑椹胚混合胚移植到6頭受體, 3頭妊娠(其中一頭母豬產仔8頭,兩頭為皮膚色素嵌合體) ,妊娠嵌合率為17 % ( 1 / 6 ) ; 126枚擴張混合囊胚移植到5頭受體均按時返情,妊娠嵌合率為0 ( 0 / 5 ) 。分享友人