標記染色體 的英文怎麼說
中文拼音 [biāojìrǎnshǎitǐ]
標記染色體
英文
marker chromosomes- 標 : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
- 記 : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
- 染 : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
- 色 : 色名詞[口語] (顏色) colour
- 體 : 體構詞成分。
- 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
- 染色體 : [生物學] chromosome染色體疾病 chromosomal disorders; 染色體異常 chromosome abnormality
- 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
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Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。Y chromosome is transmitted in the form of hap - loid, leading to extreme disequilibrium of y chromosome genetic markers distribution in different population. the prerequisite of str application in forensic medicine is establishment of a database of population y - str loci haplotype distribution. therefore we need to form haplotypes by using the known highly polymorphic str loci and detect more local population
由於y染色體呈單倍體遺傳,導致y染色體遺傳標記在不同人群中的分佈極不平衡,群體差異比常染色體str位點更加顯著,在法醫學應用的前提條件是:建立含有多個y - str位點的單倍型的群體分佈數據庫。Conclusion chromosome abnormalities may be one of the important causes of abortion, stillbirth, monster and malformation
其中常染色體異常16例,性染色體異常3例,染色體形態異常7例,標記染色體3例。Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased
腦內gfap陽性結構也明顯增多,其分佈與fos陽性細胞分佈基本一致,表現為胞體肥大、突起粗長; ast緊密包繞在神經元周圍形成神經元- ast復合體( n - asc ) ;在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ; ( 2 )免疫電鏡下son內星型膠質細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區( edas ) ,高滲刺激后數量明顯增多: ( 3 )將們個mp注入大鼠一側n卜、卜卜或孤束核( ws ) ,分別在延髓內臟帶( mvz ) 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ; ( 4 )高滲刺激使培養神經元和ast內鈣水平先升高后降低,最後維持在比高滲刺激前稍高的靜息鈣水平上。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。The ms188 gene was finely mapped. a total of 8 new indel markers were designed to map msl88 using a segregating population with a total of 2135 male sterile progenies. ms188 was finally mapped to a region of 95. 8kb between the molecular marker mda7 and k24c1
在與ms188連鎖的分子標記mc015附近設計了8個indel分子標記,對遺傳群體中2135株不育植株進行基因型分析,最後將目的基因定位於第五條染色體分子標記mda7和k24c1之間95 . 8kb的區間內。Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ,分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集,其餘胚胎的染色體則處于原核期;而只在融合后3h , 27 . 9 %重構胚被標記出組裝的紡錘體,且其中的同源染色體己經分離。The objective of the present paper was to investigate the genome constitution of three species of hystrix and their taxonomic status from cytological and molecular data. by rapd and issr assay, the present study was to evaluate phylogenetic relationships among hystrix, leymus and psathyrostachys, and to compare the use of two molecular markers in the genus and species of triticeae
本研究( 1 )通過形態學特徵比較、細胞學和繁育學資料,探討三個hystrix物種的染色體組組成及親緣關系; ( 2 )通過rapd分析和issr分析,探討hystrix與leymus 、新麥草屬psathyrostachys的屬間親緣關系; ( 3 )比較rapd和issr兩種dna分子標記在hystrix及近緣屬系統分類研究中的應用。Z which involve in melanin, tyrosinase related protein 1 gene ( tyrpi ) and dermal melanin inhibitor gene ( id ). the genomic structure of tyrp1 was determined and its correlation between melanin accumulation and tyrp1 expression was studied. moreover, we constructed a bac contig near id locus which was on a region short of dna marker in chr. z
利用構建的bac文庫對z染色體上黑色素相關基因酪氨酸酶相關蛋白1基因( tyrp1 )的基因結構以及該基因的表達與黑色素沉積的關系進行了研究,同時構建了位於z染色體上缺乏標記區段的表皮黑色素抑制因子基因( id )的bac重疊群。Using these ssr markers and hmw - gs markers, we detected the polymorphism of 111 lines selected randomly from 131 lines of ril - 8. a marker genetic linkage map with 18 chromosomes and 51 polymorphic sites covered 1296. 7 cm of wheat genome was carried out using mapmarker / exp 3. 0
利用上述標記,對隨機選擇的ril - 8群體的111個系進行了分析,採用mapmarker exp分析軟體,繪制了一張共包括18條染色體(不含1a 、 3a 、 7d ) 、 51個位點的分子標記連鎖遺傳圖譜,總長1296 . 7cm 。Short tandem repeat ( str ) is one of the genetic polymorphic markers within the nry region in chromosome y, which is also called microsatellite
短串聯重復( str )是y染色體nry區的多態性遺傳標記的一種,又稱微衛星。Y - str has many advantages over other genetic markers and it has become a hot topic of both application and research in forensic medicine
與其他遺傳標記相比, y - str具有許多優點,正成為y染色體遺傳標記中法醫學應用和研究的熱點。Objective in order to improve the discrimination power and exclusion chance of polymorphic markers on chromosome y, it is necessary to cast about for novel y - str loci and to develop a new method to genotype y - snp for forensic science
目的為了提高y染色體遺傳標記的個人識別能力和非父排除能力,尋找新的y - str和建立新一代遺傳標記y - snp的檢測方法十分必要。They were suitable for improving identification power of polymorphic markers on chromosome y. our approach of hplc can separate the oligonucleotides with different size and same size with different sequences, and provide a method to quantity products of snupe
單倍型變異度表明這8個丫str具有較好的個人識別能力和非父排除率,能夠較好地提高y染色體遺傳標記的個人識別和非父排除能力。Linkage analysis plays an important role in gene mapping. the foundation : the two gene locuses which locate on the same chromosomal ( eg. disease gene and marker gene ) happen to cross over and recombine. the farther the distance between two locuses is, the higher the probability happening to cross over is, the lower the probability that the two locuses are inherited to offspring together is, that is, the degree of linkage is not strong. so we can estimate the distance and the degree of linkage by the recombination fraction between the two locuses to locate gene
連鎖分析是基因定位主要策略之一,其基本原理是位於同一染色體上兩個基因位點(例致病基因與標記基因)在減數分裂的過程中會發生交換與重組,染色體上的兩個位點間距離越遠,發生重組的概率就越大,兩個位點在一起傳給後代的機會就越少,即連鎖程度弱,這樣由標記位點與疾病位點間的重組率可估算出兩者間的距離以及連鎖程度,達到基因定位的目的。Kenyon cells are not labelled. the calyces and pedunculus of mushroom body and the a - lobe and 3 - lobe show gaba - like immunoreactivity. the staining of a - lobe are deeper than p - lobe
Kenyon細胞未被標記,蕈形體冠、柄、葉和葉卻顯示gaba免疫活性,葉染色比葉深。The mutation will still be embedded in a very long section of the founder ' s version of dna after only one recombination, just as a marked card would still be accompanied by many of the same cards that were around it in its original deck after only one rough cut - and - mix
經過一次染色體重組后,突變就會位在很長一段和創始者dna相同的區域內,就像在一次粗略洗牌后,帶有標記的那張撲克牌前後仍有很多來自同一副的牌。They are currently developing an expression system containing the antibody gene, devoid of antibiotic selection markers, and integrating the system into the chromosome of lactic - acid bacteria, with the goal of achieving passive immunization at mucosal surfaces
他們正在開發一種含有抗體基因表達系統,無抗性選擇標記而系統整合到乳酸菌染色體的體系,以實現粘膜表面的被動免疫。A mapping analysis revealed that ast gene was located on chromosome i, and located between sslp molecular marker nga280 and caps molecular marker pab5
初步作圖分析表明, ast基因定位於擬南芥第在號染色體上,並且位於sslp分子標記nga280和caps分子標記pab5之間。Using the test of single environmen, 38 qtls of additive effects distributing on 16 chromosomes were obtained. the range of contribution rate in different single qtl is 5. 08 - 19. 89 % ; 52 interaction effects distributing on 17 chromosomes were obtained. the range of contribution rate in different single interaction effect is 4. 51 - 57. 14 %, with contribution rate of 57. 14 % of interaction effect between locus 3d - 1 and locus 7b - 1 in environment 4
在不同環境分別進行分析下,檢測到9個性狀的38個次加性效應qtls ,分別位於16條染色體,單個qtls貢獻率變化范圍為5 . 08 19 . 89 ;檢測到9個性狀的52對次qtls互作效應,位於17條染色體,單個互作效應的貢獻率變化范圍為2李斯深:小麥產量性狀qth的分子標記定位4分享友人