次生培養細胞 的英文怎麼說

中文拼音 [cìshēngpéiyǎngxìbāo]
次生培養細胞 英文
secondary culture cell
  • 次 : Ⅰ名詞1 （次序; 等第） order; sequence 2 [書面語] （出外遠行時停留的處所） stopping place on a jou...
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  2. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  3. Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed

    摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。
  4. Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall, and agrobacterium / " / z / zogerae. s ' - transformed cell line of medicago saliva l. using protoplast fusion by peg method

    通過peg誘導原生質體融合和雜種細胞的篩選培養，首次得到沙打旺（ + ）紫花苜蓿的屬間體細胞雜種。
  5. In this report, we mainly covered the following aspects of " tissue organ regeneration and replication in situ " : 1 ) procedures of tissue organd regeneration and replication and replication in clnical practice ; 2 ) the discover and existence of potentiald regenerative cell ( prc ) ; 3 ) the proliferation, differentiation and regeneration law of potential law of potential regenerative cells ; 4 ) study procedure on tissue organ regeneration and replication from prcs in vitro based on the model of full skin organ regeneration in situ after extensive in vitro, set up the method and technology of searching life regenerative substance required in tissue organ regeneration and replication in situ. in this study, first, the whole human body is divided into 206 function units, which are the " tissue organ " in regeneration study. then the histology foundation of tissue organ regeneration and replication in situ is set up. in ordre to prove the existence of the potential regenerative cells and their potential baility and function, we established clinical tracking rechnique of skin organ regeneration in situ ; meanwhile, several tissue organ regeneration and replication in vitro models which represent different kinds of runctions were sucessfully set up, with all these techniques and models, we confirmed : 1 ) the existence, function and ability of pptemtoa regenerative cells ; 2 ) the importance of life regenerative substance ; 3 ) the feasibility of tissue organ regeneration and replication in situ ; 4 ) the big value of tissue organ regeneration and replication in situ in life science and medicine progerss. we also showed the possible foreground of capture cancer with this method and technologh. in this report, nearly 200 photographs of several tissue organ regeneration and replication in situ or in vitro demonstrated the whole process of tissue organ and big organ entities regeneration and replication from cells. the results of tissue organ regeneration and replication in situ mainly include : 1 ) whole skin organ regeneration and replication in situ ; 2 ) gastrointestinal mucosa tissue organ regeneration in vitro ; 3 ) hair follicle tissue organ regeneration in situ or in vitro ; 4 ) never tissue organ regeneration in situ ; 5 ) pancreas tissue organ regeneration and replication in vitro ; 5 ) marrow tissue regeneration in vitro ; 6 ) renal glomerulus and tubule tissue organ tugeneraation in vitro ; 7 ) heart muscle regeneration in vitro, etcl. in order to let more and more people know and understand this technology of tissue organd regeneration and replication in situ, herein, for the first time, we publicize the key points of actualizing this technology. also, we publicized the technology procedures and the frame constitute of life substances. we bilieve this is a big contribution to human science

    本研究報告，重點報道了組織器官的原位再生復制的臨床程序，報道了組織潛能再生細胞的發現和存在，以及該細胞的增殖分化和形成組織器官的變化規律.以燒傷后皮膚組織器官的原位再生復制為模型，研究出了體外組織潛能再生細胞復制組織器官的培養方法;以體外組織器官的復制為模型，建立了尋找原位組織器官再生復制所需生命物質的方法和技術.本研究，首先按人體的器官功能，分解為206個功能單位，確立了所復制的人體器官中的組織功能單位為組織器官，從而建立了原位組織器官再生復制的組織學基礎.為了驗證組織潛能再生細胞的再生潛能，建立了皮膚器官原位再生的實體臨床跟蹤技術，同時又建立了能代表有關器官功能類別的代表組織器官的原位和體外復制模型，以多組織器官的成功復制確定潛能再生細胞的作用，確定生命研究再生物質的重要性，確定組織器官原位再生復制的可行性，確定了組織器官原位再生復制的生命科學研究和醫學進步的重大應用價值，同時展示了用此方法和技術攻克癌癥的前景.本研究報告，以近二百幅多個組織器官原位和體外再生復制的實體圖片，展示了潛能再生細胞復制的組織器官和大器官司實體;展示了細胞再生復制器官的全過程.真實的報告了組織器官原位再生復制的成果.所公布的主要成果為:皮膚器官的原位再生復制;胃腸黏膜組織器官的原位和體外再生復制;毛囊組織器官的原位和體外再生復制;神經組織器官的原位復制;胰腺組織器官的體外復制;骨髓組織的體外復制;腎小球小管組織器官的體外復制;心肌的體外復制等.為了讓更多的人學會和掌握組織器官原位再生復制技術，本報告首次公布實施技術的重要環節和技術流程;首次公布了生命再生物質的框架和組成.作者自費研究成果對人類生命科學的一大貢獻
  6. After inoculation, all strains were examined by microscopy, hyphae or cells of all strains were observed, but none was seen in negative comparison. in the same time, originally fungi were isolated again in sabourud which showed that these fungi could grow and reproduce in these animals, but if they can cause infection or not will be make sure with impressionable animals

    回接后所有實驗菌種在顯微鏡下均可見有菌絲或菌細胞生長，而陰性對照組則未見生長，同時可以從沙堡氏培養基中再次分離得到該菌，說明這些菌種均可以在動物體內生長繁殖，但是否能真正引起感染，還需要進一步使用易感動物進行確認。
  7. The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin

    從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞，經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上，培養基中添加了lif ， bfgef ， foskolin等細胞因子，此後大約每7天左右傳代一次。
  8. 3 in the light and dark, the contents of soluble protein, the activities of peroxidase ( pod ), superoxide dismutase ( sod ) and esterase ( est ) were studied, and they all showed their changes respectively. 4 the alkaloids were accumulated primarily in the stationary phase

    4 、培養細胞中次生代謝物質的積累：光照和黑暗條件下生物堿的含量變化不盡相同，但第12d均出現一個峰值，並且從第18d起生物堿的含量呈上升趨勢。
  9. Abstract : the diferrences in cell growth, composition and levels of anthocyanins and catechins, activity of phenylalanine ammonia - lyase and dynamics of production between anthocyanin accumulating and non - accumulating subclones of tea cultured cells were examined

    文摘:從細胞生長、色素和兒茶素次生產物組成與含量、苯丙氨酸解氨酶活性以及產物合成動態分析幾方面，研究比較了積累色素與不積累色素的兩類茶樹培養細胞系之間的差異。
  10. Preparation requires isolating the patient ' s white blood cells and then separating out a mix of dendritic cells and precursor cells still undergoing maturation ( both types may produce an immune reaction )

    其準備功夫包括先分離病人的白血球，然後從中分離出樹突細胞以及未成熟前驅細胞的混合物（這兩種細胞都可能產生免疫反應） ，再與融合蛋白一起培養40個小時，之後才注射回病人身上；這個步驟一共要重復三次。
  11. The growth circle and some physiology - biochemical indexes were illustrated. as the same time, the tendencies of flavonids, saponins and tea - polyphenols during growth circle are analysed. the paper provides scientific and experimental basis for cell culture and secondary metabolism matters yield

    分析了在愈傷組織生長周期中山茶總黃酮、總皂甙和茶多酚的含量變化，為山茶的進一步開發，細胞培養和次生物質的生產提供理論和實驗依據。
  12. Development system in some streptomyces spp. is characterized by an unique multiphasic growth kinetics in liquid medium. after spore germination, first logarithmic growth is followed by a transient cessation of cell growth, which potentially serves as a key developmental switch in regulatory system, leading to initiation of second logarithmic growth

    某些鏈黴菌在液體培養基中表現獨特的多相生長動力學特徵：第一次對數生長之後，細胞生長過渡性地停止，這個過渡期可能起著一個重要的發育調控開關作用，啟動細胞第二次對數生長。
  13. 2. the plasimd encoding human carcinoembryonic antigen was transfected with lipofectine into human dcs and human tumor cells such as mgc803, ls174t and eel09. the culture supernatants were collected for assaying carcinoembryonic antigen by. 3

    1640培養液中，在37ac ， 5 co ，的培養箱內培養， 』每3心傳代一次，取對數生長期的細胞進行實驗。
  14. It was observed that micro - organisms in soil treated with the herbicide at a range of 2 - 50 mg / kg grown greatly different from those in control. the results showed that bacteria, such as bacillus and pseudomonas were grown most fast ; and then staphylococcus, vibrio and escherichia were increased obviously ; but that actinomyce and fungi were seemed to be inhibited by quizalofop - p - ethyl

    土壤中的細菌（ bacteria ）種群數量在70d培養期內與對照組相比均有不同程度增加，其中芽孢桿菌（ bacillus ） 、假單胞桿菌（ pseudomonas ）的生長最快，弧菌屬（ staphylococcus ） 、埃希氏菌屬（ hibrio ）和葡萄球菌屬（ escherichia ）的生長速度次之。
  15. We determine the growth process and mutual relationship of three bacteria, i. e. photosynthetic bacteria, e. coli ki2 and etec 987p. relationship between pathogenic bacteria in water, aeromonas hydrophija, and phoiosynthetic bacteria was studied emphatically. the result showed that when inoculating quantity of phoiosynthetic bacteria was one fifth of that of aeromonas hydrophila, the growth of latter bacteria could be inhibited distinctly

    其次，研究了混合生長體系的細菌色譜行為，實時對混合培養體系中每一種細菌的生長情況作出定量和定性的分析，確定了光合細菌、 e . colik12和etec987p三種細菌混合生長的過程及相互競爭與抑制的作用關系；針對水體中病原菌? ?嗜水氣單胞菌，重點考察了它與光合細菌的作用關系，結果表明光合細菌的接種量為氣單胞菌的1 5時，就能夠對氣單胞菌有明顯的抑制效果。
  16. The studies on cell immobilization culture of ginkgo biloba l, as well as the production of their secondary metabolite - ginkgolides were reported in this paper

    本文對銀杏細胞固定化培養及其次生代謝產物銀杏內酯的產生進行了較為系統的研究。
  17. Research advances on synthesis of secondary metabolities by plant cell culture

    植物細胞培養技術合成次生代謝物質研究進展
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