止桿 的英文怎麼說
中文拼音 [zhǐgǎn]
止桿
英文
stopping lever-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The glass insulator prevents any charge from leaking off the support and gold leaves.
玻璃絕緣體防止任何電荷從金箔及其支持桿漏跑。Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g酰化酶( afpga )基因構建表達載體pkkfpga和psmlfpga , pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子,其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子;而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。Step down the bottom rocker rail and move the stop lever rightwards. the stop lever will lock rocker and you can easily lift the wringer
踏下擺臂橫桿並向右撥動止動桿,止動桿會自動將擺臂鎖在兩滾筒合攏位置,此時可用手握住提手提起擠水器。To prevent magnetic leak, improve the abrasiveness and the anti - eroding ability, the author have spent lots of time in researching the hot spraying method and acquired many practical useful data and experience
為了防止泄漏,增加活塞桿的耐磨性和抗腐蝕性,本文還對熱噴塗工藝進行了研究,並摸索出了大量實際有用的數據及經驗。A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon
在其起始密碼子上游- 65nt發現桿狀病毒晚期啟動子轉錄起始信號ataag ,在其終止密碼子下游- 14nt發現polya加尾信號aataaa 。Ensure the torsion bar is fitted in the correct position to prevent contact with the suspension frame
確保扭力桿安裝在正確的位置以防止與懸架摩擦。Structural reinforcing rods protect cable from excessive bending strain and compressive stresses, and the current transfer tab is designed for economical grounding by providing direct contact with opgw, accommodating a 1 2 " bolt and nut
光纜固定採用與光纜直徑相匹配的鋁管夾緊,防止夾傷光纜,根據需要,可安裝在桿塔的不同部位,安裝操作極為方便。Stem : the tee - head disc - stem connection prevents lateral strain on the stem for smooth, easy operation. accurately cut threads engage the yoke sleeve for positive control of disc position
閥桿: t形閥瓣-閥桿連接防止施加在閥桿側面的應力,確保操作平穩順暢容易。軛架套管準確的螺紋連接有助於閥瓣位置的調整控制。Compared hasnpv helicase with the helicases of autographa colifornica mnpv ( acmnpv ), ( bombyx bori npv ( bmnpv ), lymantria dispar mnpv ( ldmnpv ) spodoptera exigue mnpv ( semnpv ), orgyia pseudotsugata mnpv ( opmnpv ), and xestia c - nigrum granuloviurs ( xcgv ), only 5 motifs ( i, la, ii, iii, iv ) were found conserved in baculovirus
其在終止密碼子下游第12位有? polya終止信號aataaa 。 hasnpv解螺旋酶與其它5種桿狀病毒解螺旋酶相比,有5個基元序列( 、 a 、 、 、 )保守,另外兩個(和)完全不保守。When the torque was being increased, this equipment would quickly measure the data of torque, making electromagnetic clutch opened and feed stopped, so that the driller and drilling bar would be protected
當扭矩增大時,可以快速檢測扭矩參數,並控制離合器迅速脫開,停止進給,保護鉆頭鉆桿。The company is specialized in the production of small diameter valve suitable for electrical station as well as sampling device and automatic feeding device for firepower electric plant, stop valve for high temperature and high pressure condition, throttle valve for high temperature and high pressure condition, counter - pressure high efficiency cooler, bi - screw reducer, fine adjustment valve together with pipe break, stop valve, diaphragm valve, plug - in speedy connector, ball valve and various special valves in addition to meter connector
公司主要專業生產小口徑電站閥門及火力發電廠的爐內化學檢測集中取樣裝置、爐內自動加藥裝置,高溫高壓截止閥,高溫高壓限流閥,反壓式高效冷卻器,雙螺桿減壓閥、微調閥等取樣裝置件和石油化工行業使用的管道閘閥,截止閥,隔膜閥,承插式快速接頭,球閥及各種特殊閥門,儀表接頭等。Add railings at jockey club road and man tung road to prevent jaywalking
在馬會道及文東路加設欄桿,以防止行人亂過馬路。Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay
由於vgb基因啟動子不能在枯草桿菌中啟動表達,因此,根據已發表的果聚糖蔗糖酶基因( sacb )序列設計引物,從枯草桿菌wb600總dna中擴增出該基因的啟動子片段,然後將其與vgb基因編碼區及終止子序列相連,成功地組建了sacvgb融合基因。Note : on pump discharge, the preferred check valves are an in - line spring loaded, swing design with lever and weight or lever and spring
注意:在泵的排泄口處,首選的止回閥是管內彈簧負載,旋啟式,配置杠桿和重量或杠桿和彈簧。Annular balance whip
環狀擺輪止動桿A catch or lever that locks the movement of one part of a mechanism
制動裝置阻止機械裝置的某一部分的運動的擒縱裝置或控制桿Automatic computation and modification of the dimension, the number of total heat tubes and rows, the body line of layout areas and other results involved. 4. the programming of various functional models and their communication, coded with visual lisp and vba and based on the opening structure of autocad and its entity database
標示最大布管邊界,自動標注尺寸,自動修改並輸出布管參數及結果,包括:自動計算並輸出每一管程的管根數、起始排數、終止排數,自動計算並輸出每排熱管根數與拉桿根數。Locking the rotation of the laminar coupling ( 2 ) with the reaction lever 99370317 ( 1 ) and using a suitable wrench unscrew the retaining screws ( 3 ), remove the laminar coupling and the flange
利用止動桿99370317 ( 1 )鎖定迭片式聯軸節( 2 )不讓其轉動,並且使用適用的扳手鬆開固定螺釘( 3 ) ,將迭片式聯軸節和法蘭卸下。Brake lever stop
閘杠桿止動器分享友人