母體化合物 的英文怎麼說
中文拼音 [mǔtǐhuàgěwù]
母體化合物
英文
index compound- 母 : Ⅰ名詞1 (母親) mother 2 (泛指女性長輩) one s elderly female relatives 3 (配套的兩件東西里的凹...
- 體 : 體構詞成分。
- 合 : 合量詞(容量單位) ge, a unit of dry measure for grain (=1 decilitre)
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
- 母體 : 1 (孕育幼兒的人或雌性動物的身體) the mother s body; the (female) parent; parent body 2 [原子...
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In the course, the condition and the process of forming and changing of minerals, the typomorphic features of minerals, the characteristics of the distribution and the evolution of minerals and their paragenetic associations in the period and the space, the methods of mineral analyses and research, the geological thermometers and the geologicial barometers of minerals, mineral paragenetic associations and paragenesis analyses will be introduced, and the magnificent and the microscopic genetic characteristics of pyrite, quartz, garnet, pyroxene, amphibole, mica, feldspar, and other minerals will be dealt with particularly
本課程主要講述礦物及其共生組合的形成和變化的條件和過程,不同物理化學條件下礦物的成分、結構、形態、物性等標型特徵以及它們之間的內在聯系,礦物及其共生組合在時間上和空間上的分佈和演化規律,礦物成分、結構、形貌、物性等的分析測試方法,礦物地質溫度計和地質壓力計對其形成環境的表徵,不同體系(封閉體系、開放體系)條件下,礦物的穩定范圍和彼此替代順序的共生分析,重點介紹黃鐵礦、石英、石榴石、輝石、角閃石、雲母、長石等礦物(族)在成因上的宏觀標志和微觀信息,以及其成因信息在成巖作用和成礦作用中的地質意義。Yeast elongator was isolated as a complex that associates with the chromatin fraction and interacts with the elongating phosphorylated form of rnap ii. the functional entity of elongator complex has recently been shown to be an unstable six - subunit complex, termed holo - elongator, which can dissociate into two discrete three - subunit subcomplexes upon treatment with high salt and / or monoq chromatography. one of these subcompexes is the elp3 - containing core complex, and the other is a complex of the elp4, elp5 and elp6 proteins
酵母elongator是與染色質組分結合,並與延伸中的磷酸化形式的rnap相互作用的復合物,是個六亞基的功能整體,在高鹽處理或monoq層析時易於分解為兩個三亞基亞復合物,其中一個是含elp3的核心復合物,另一個含elp4 5 6亞基。The actual compound is considered to be a derivative or the parent compound.
把實際化合物看作母體化合物的衍生物。9 grinding and cleaning of alloy steels, stainless steels and nonferrous metals shall be done only with tools that will not leave detrimental deposits on the base metal ; aluminum oxide or silicon carbide grinding wheels and austenitic stainless steel wire brushes shall be used
合金鋼、不銹鋼和非鐵金屬的研磨、清理所用工具不能在母材金屬表面上留下有害殘留物,可為鋁氧化物或金砂鋼磨輪、奧氏體不銹鋼絲刷。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程,在分子水平上它包括對外界鹽信號的感應和傳遞,特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中,本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來,通過對各種鹽生植物或鹽敏感突變株的研究,闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑,克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面,在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因,這些都有助於闡明植物鹽脅迫應答的分子機制。The recombination expression vector, ppic9 - e2, was linearized by sal i and electroporated into p. pastoris gs115. recombinant p. pastoris gs115, designated gs115 - ppic9 - e2, was identified by pcr and the product of the pcr was analyzed by sequencing before its expression for csfv e2 protein
重組表達載體ppic9 - e2經sal酶切線性化后,電轉化整合到畢赤酵母gs115基因組上,經pcr鑒定和pcr產物測序分析,陽性轉化子命名為gs115 - ppic9 - e2 。This measures are, namely, to set up parent - subsidiary company system in chinese tobacco group head office, to unify technique development, to unify the arrangement of the cigarette productivity, to increase the brand concentration, to unify the build of the cigarette marketing network and the logistic sending system, to unify the fund management, to unify the strategy measures to reasonably arranging tobacco resources, so as to promote the overall ability of chinese tobacco head office to compete, all in a bid to build china tobacco ' s core competency
然後,本文從戰略與環境及資源能力相匹配的角度,規劃了中國煙草總公司集團化經營的基本運作模式和組織結構調整方案,提出了增強中國煙草總公司競爭能力的戰略措施,即:組建母子公司體制的中國煙草集團總公司,統一進行技術研發,統一進行卷煙生產力布局,提高品牌集中度,統一進行卷煙營銷網路和物流配送體系建設,統一進行資金管理,統一進行煙葉資源合理配置等戰略實施措施,進而全面提升中國煙草總公司的核心競爭能力。Experiment shows that tpsl gene can endow organism the ability of synthesis trehalose, the dephosphorylation of the trehalose - 6 - phosphate is not special, and it can be replaced by other phosphatases. the tpsl gene from saccharomyces cerevisiae was cloned by pcr amplification
實驗證實tps1基因就可以使生物體獲得產生海藻糖的能力,酵母的海藻糖合成酶復合體中6 -磷酸-海藻糖的脫磷酸化作用是非特異性的,它可由生物體內的其它酯酶所代替。The soil organo - mineral complexes, formed by intimate association of organic matters or humic substances and mineral, is one of the fundamental features that distinguishes soils from their geological parent materials, and effects on the nature of soil aggregate, and the amounts and characters of soil particle aggregation is closely related to soil fertility. by way of collecting soil samples outdoors and analysis indoors and using the dry land soils developed from purple soils as research materials, this paper investigated status of organo - mineral complexion, characters of aggregation and soil fertility for discussing the relationship among them under different land treatments, and the factors that influencing the amounts and characters of organo - mineral complexion and soil aggregation and restricting status of soil fertility, the main results were as follows : there were remarkable differences of status and activity of fe oxide and aluminum oxide in dry land soils developed from different parent soils and under different utilizations
土壤中有機物質和礦物質的結合?土壤有機無機復合體是土壤區別其母質的基本特徵之一,它對土壤團聚體的性質有很大的影響,而團聚體的數量和性狀有跟土壤肥力密切相關,本文以不同母巖發育的旱地紫色土作為研究材料,通過野外采樣和室內分析,研究了旱地紫色土的有機無機復合狀況、團聚體特徵和肥力水平,探討了在不同土地利用方式下它們之間的關系,以及影響有機無機復合、土壤中團聚體的數量、性質以及制約土壤養分狀況的因素,主要結果如下:不同類型的紫色土鐵鋁氧化物形態和活性具有很大差別。Abstract : a review with 6 references is given on some formatities of trace element supplement for people including natural foods, inorganic chemicals, organic chemicals, complex trace element drugs and trace element ferments
文摘:論述了人體補充微量元素的幾種主要方式,其中包括天然食物、無機化合物、有機化合物、復合微量元素藥物和微量元素載體酵母等。Under the induction by methanol, the expressed monellin was secreted into medium at the level of 0. 25g / l. the gene ( vgb ) coding for the vitreoscilla hemoglobin ( vhb ) molecule was cloned and funtionally expressed in pichia pastoris. shake flask fermentation experiments showed that the presence of the intracellular vhb in pichia pastoris efficiently enhanced cell growth under oxygen - poor conditions
同時,採用畢赤酵母偏愛密碼子,人工合成了透明顫菌血紅蛋白基因( vgb ) ;並構建了vgb基因的酵母胞內表達載體ppic3 . 5kv ,通過電擊轉化獲得了能在胞內表達有生物活性vhb的重組畢赤酵母gs115 / ppic3 . 5kv 。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變, pcr產物與ppic3 . 5k連接,轉化大腸桿菌,獲得的混合質粒電轉化畢赤酵母gs115 , omm平板篩選適于低溫或對熱穩定的重組子,篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ,其最活反應溫度為45 ,比野生型高出5 ; 40處理30min殘留活性為56 ,大大高於野生型的6 ;初始ph7 . 3528條件下培養72h ,發酵上清酶活為325u / ml 。We also constructed ppiczaa - a - 1bgp vector, and transformed it into gs115, then confirmed the mut phenotype, examined the presence of insert using pcr, conducted small - scale expression, analyzed the expression by sds - page and western blot
另構建了ppiczaa a叫酵母表達載體,轉化gslls菌株,確定表型, pcr篩選a ibgp有效整合酵母基因組的菌株,進行誘導表達, sds一page凝膠電泳和westernblot鑒定表達產物。分享友人