毒性基 的英文怎麼說
中文拼音 [dúxìngjī]
毒性基
英文
toxophoric group-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library
我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法，獲得了對酵母細胞即無毒性作用，又沒有自激活作用的「誘餌」 ，通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。Standard guide for conducting renewal microplate - based life - cycle toxicity tests with a marine meiobenthic copepod
用海洋meiobenthic橈足類甲殼動物進行復原的基於微板的壽命周期毒性試驗的標準指南Abstract : numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
文摘:許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料Numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料Neither teratogenic nor embryotoxic effect were observed in rats given stevioside at doses of up to 1000mg kg of body weight per day by gavage. the results of genotoxicity tests with stevioside in various
在倉鼠身上，配基甜菊醇的毒性較甜菊糖劇烈，但這現象沒有在大鼠身上出現。Neither teratogenic nor embryotoxic effect were observed in rats given stevioside at doses of up to 1000mgkg of body weight per day by gavage. the results of genotoxicity tests with stevioside in various
在倉鼠身上， ?配基甜菊醇的毒性較甜菊糖?劇烈，但這現象沒有在大鼠身上出現。Characterization of hemagglutinin gene of influenza a virus subtype h9n
毒株基因組特性的研究Expression of thermostable direct hemolysin gene of vibrio parahaemolyticus in e. coli and activity of expressed product
副溶血性弧菌直接溶血毒素基因的原核表達及產物的性質In one word, the methods for producing acellulace matrix have not been standardized. in this study, we want to select the best way to produce acellular matrix. we also use immunohistochemical method for detecting the magor heterogenetic antigen a - galactosyl residues ( a - gal ) in acellular matrix
並應用免疫組化的方法檢測脫細胞后基質的主要異種抗原，用種植實驗觀察異種機體對脫細胞生物基質的組織反應倩況，另在其上種植鼠成纖維細胞以觀察脫細胞生物基質的細胞毒性和細胞相容問題。From different points of view of the epidemiology, cytogenetics, molecular biology, experimental pathology and preventive medicine, this study revealed and proved that the genotoxicity and carcinogenesis existed in the drinking pond water in the high incidence area of live cancer in guangxi, indicated the feasible preventive and interruptive methods, too
從分析流行病學，細胞遺傳毒理學、分子生物學、實驗病理學和預防醫學的不同角度，揭示和論證了廣西某肝癌高發區居民飲用塘水的基因毒性和致癌性，提出了切實可行的預防和阻斷措施。The “ genabon ” series product has : the environmental protection foam rubber paint, water wooden vessel paint, polyester repairs the paint, flexibility texture suffers the coating and have no the benzene poison nitric paint, furniture industry uses the paint and have no the benzene all - powerful gum, refractory coating, arrest burnt two use to repair the paint and have no the not poisonous condensate in benzene turn, have no the benzene dilution a printing ink, oil building coating, ground ping paint, street nark line the paint, industry antisepsis coating etc
「金耐寶」系列產品有：環保乳膠漆、水性木器漆、聚酯裝修漆、彈性質感受塗料、無苯毒硝基漆、傢具工業用漆、無苯萬能膠、防火塗料、阻燃兩用裝修漆、無苯無毒固化劑、無苯稀釋劑、特種油墨、油性建築塗料、地坪漆、馬路標線漆、工業防腐塗料等。Parkinson ' s disease arises from genetic and possibly neurotoxic causes that produce massive cell death of the neuromelanin - containing dopaminergic neurons of the substantia nigra
帕金森氏病的病因包括基因突變和神經毒性物質作用，導致黑質多巴胺能神經元大量的死亡。It normally exists as a nontoxic linear chain of amino acids
正常情況下以無毒性的鏈條狀氨基酸存在。Two kinds of compound ( nonylphenol and octyl phenol ) can not only decrease the number of the reproduction, but also affect the parthenogenesis of daphnia magua straus
結果表明，急性毒性較大的烷基酚，例如4 -辛基酚和4 -壬基酚對大型蚤的生殖毒性產生影響。Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2
含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。We have reported that squalene, a natural fish oil, is a potential preventive agent against sodium arsenite - induced sister chromatid exchanges and micronuclei in chinese hamster ovary - k1 cells
以達成熟地步，因此使得探討環境毒物暴露引發的差異性基因表達成為可以進行的研究。我們已開始以In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg
同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。分享友人