毒性桿菌 的英文怎麼說

中文拼音 [dúxìnggǎnjūn]
毒性桿菌 英文
toxic bacillus
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 桿 : 桿名詞（桿子） pole; staff
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 毒性 : [藥理學] toxicity; virulence; poisonousness毒性測定 toxicity test
  • 桿菌 : [微生物學] bacillus
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. Type 1 pili is the important virulence factors on the e. coli in fection in chicken. through the adhering of pili, e. coli adhered on the epidermic cell of aspiratory tract, which was the first step of invading in host

    1型菌毛是雞源致病性大腸桿菌的重要毒力因子，在致病過程中介導細菌吸附於雞呼吸道粘膜上皮細胞完成入侵的第一步。
  4. It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin

    在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考
  5. Biogen - tab is an effective and invaluable medicine for many poultry diseases, particularly chronic respiratory disease, infectious coryza, salmonelloses fowl typhoid and paratyphoid infection, blue comb disease, fowl cholera, coccidiosis, and leucocytozoon disease. the diseases infected by virus, such as newcastl disease, avian encephalomyelitis are found not to be effected by biogen - tab treatment

    愛禽美對許多家禽疾病是相當有效的藥品，特別是慢性呼吸器病，通稱crd ，傳染性可利查，沙士桿菌病如家禽傷寒，副傷寒，藍冠病，家禽霍亂，球蟲病，及白冠病等但有些疾病，由病毒感染者無效如新城雞瘟，傳染性喉頭氣管炎
  6. Data from the symposium entitled, " clostridium difficile - associated disease : underdiagnosed, underreported, undertreated ; how to oercome the challenges, " confirmed the emergence and spread of a new irulent epidemic strain of cdad known as north american phenotype 1 / 027 ( nap1 / 027 )

    標題為「難治性梭狀芽孢桿菌相關疾病：診斷不充分，報道不全，治療不足；如何來戰勝這個挑戰」的這個專題討論會證實了一種叫做北美1 / 027表型的新的毒力和傳染性較強的菌株的出現和傳播。
  7. The high specificity of the dot - ppa - elisa was confirmed by specific blocking test and also by cross - reaction test. the diaphragm did not react with the antibodies against salmonellosis, streptococcosis, colibacillosis, chlamydiosis, hcv, ppv, prv, brucellosis. erysipelas, suis and chlamydiosis in cross - reaction test. the diaphragm has good sensitivity and could detect some pasteurella - positive test serum which has been diluted to2 - "

    試驗證明所建立的dot - ppa - elisa具有較好的特異性，與豬瘟、仔豬副傷寒、豬丹毒、豬細小病毒病、豬偽狂犬、豬布氏桿菌病、豬衣原體病陽性血清無交叉反應。
  8. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  9. It is also more virulent because the inoculum required for septicum to cause an infection is ~ 300 times lower in mice compared with perfringens

    小鼠實驗證實梭狀芽孢桿菌比產氣莢膜桿菌的毒性更強，因為前者造成感染的接種量僅為後者的約1 / 300 。
  10. Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2

    含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。
  11. " angelo " sterilization gel is highly effetive while mild and safe to human body with no irritation, no - toxicity and no side effect. it can instantly kill various disease - causing germs such as monilia, trichomonad, mycoplasma. chlamydia, mycete, staphylococcus aureus, dipolcoccus gonorrhoeae

    「安潔樂」消毒凍膠可迅速殺滅引起女性宮頸炎、陰道炎等皮膚粘膜感染的各類致病菌：如滴蟲、黴菌、淋病雙球菌、金黃色葡萄球菌、綠膿桿菌、白色念珠菌、衣原體、支原體、梅毒螺旋體等，對人體無毒副作用。
  12. A dh spokesman said that the sources of water and food in the affected areas might have been contaminated and could lead to food poisoning as well as food and water borne diseases, such as bacillary dysentery and cholera

    ?生署發言人表示，受海嘯影響地區的水源及食物或已受到污染，有可能導致食物中毒或其他經由食物及食水傳染的疾病，例如桿菌性痢疾和霍亂。
  13. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  14. Iraq ' s procurement efforts include equipment that can filter and separate micro - organisms and toxins involved in biological weapons, equipment that can be used to concentrate the agent, growth media that can be used to continue producing anthrax and botulinum toxin, sterilization equipment for laboratories, glass - lined reactors and specialty pumps that can handle corrosive chemical weapons agents and precursors, large amounts of vinyl chloride, a precursor for nerve and blister agents, and other chemicals such as sodium sulfide, an important mustard agent precursor

    伊拉克采購的設備包括可過濾和分離生化武器中微生物和毒素的設備；可用於為炭疽病毒和肉毒（桿）菌病毒集中藥劑和生長媒體的設備；實驗室殺菌設備；可處理腐蝕性化學武器藥劑、前體、乙烯基氯化物（一種神經和水泡藥劑）及其他化學藥劑（如鈉硫化物，芥子氣藥劑的前體）的玻璃線紋反應堆和專業水泵。
  15. Research progress of molecular biology and genetic engineering vaccine of enterotoxic escherichia coli

    腸產毒性大腸桿菌分子生物學及基因工程疫苗的研究進展
  16. The toxin, produced by certain strains of e. coli bacteria, has been found to be responsible for an outbreak of haemolytic uraemic syndrome, a dangerous disease that causes acute kidney failure, in south australia in 1998

    此毒素由大腸桿菌特定菌株產生， 1998年澳大利亞南部爆發的溶血性尿毒綜合癥（可導致急性腎衰竭的一種危險的疾病）就是由該毒素引起的。
  17. The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody

    該法以硝酸纖維素膜為固相載體，包被膜載抗原製成的診斷膜片具有良好的特異性：不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應；膜片具有良好的靈敏性，陽性血清作2 ~ （ - 11 ）稀釋亦能檢出；膜片具有良好的穩定性，在4至少能保存7個月，其靈敏性不變。
  18. Colibacollosis serotype k. 99 positive sera in ducks and duck plague positive sera were tested, whereas, the results of detection to r. a. sera were positive. the sensitivity of indirect elisa were 50 to 100 times ( serotype 1 ), ( 2 ) 25 to 100 times ( serotype 2 ), ( 3 ) 12 to 100 times ( serotype 4 ) and 25 to 200 times ( serotype 5 ) th an micro - agglutination test

    包被鴨疫里默氏桿菌抗原時，對鴨抗5 ： a多殺性巴氏桿菌陽性血清、鴨抗型鴨病毒性肝炎陽性血清、鴨抗鴨源大腸桿菌k _ （ 88 ）陽性血清、鴨抗鴨源腸炎沙門氏菌陽性血清、鴨抗鴨源大腸桿菌k _ （ 99 ）陽性血清、鴨抗鴨瘟陽性血清檢測結果呈陰性。
  19. Acute toxicity and dermal sensitzation of bacillus thuringiensis original drug

    蘇雲金桿菌的急性毒性及致敏實驗觀察
  20. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
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