毒物鑒定 的英文怎麼說

中文拼音 [dúwùjiàndìng]
毒物鑒定 英文
identification of poison
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 鑒 : Ⅰ名詞1 （鏡子 古代用銅製成） ancient bronze mirror2 （可以作為警戒或引為教訓的事） warning; objec...
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 毒物 : toxic; toxicant; poison; poisonous substance
  • 鑒定 : 1 （評語） appraisal （of a person s strong and weak points） 2 （評定） appraise; identify; auth...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  3. Based on the survey of viruses infecting araceae plants from in zhejiang province, hunan province, hainan province and other regions in china, pathogenic viruses of this family were detected and identified. sequence similarity was compared between dasheen mosaic virus ( dsmv ) isolates of different origin. virus - free seedlings were obtained for two important taros cultivar, colocasia esculenta cv

    本研究論文對我國浙江省、湖南省和海南省等地的天南星科植物上的主要病毒進行了檢測和分子鑒定，比較了該科主要病原病毒芋花葉病毒（ dsmv ）不同分離物的序列同源性並分析其種內分化關系；同時，我們對2種重要的芋屬栽培作物? ?奉化芋艿頭、烏梗芋（ colocasiaesculenta ）和我國特有的天南星科藥用植物? ?掌葉半夏（ pinelliacordata ）進行了脫毒培養和快速繁殖研究。
  4. Phytoalexins are low molecular weight chemicals that plants produce and accumulate in response to infection especially of fungal origin. sakuranetin is a kind of flavanone phytoalexin isolated from ultraviolet - irradiated rice leaves. recent research work on flavanone phytoalexins represented by sakuranetin is reviewed. interesting novel structures, stucture - activity relationships and synthetic methods are discussed

    植物抗毒素是植物受到外界病原微生物侵擾后所產生並積累的一類具有抗菌活性的小分子物質，櫻花素是從水稻稻瘟病感染組織中分離鑒定的一種黃烷酮類植物抗毒素.對以櫻花素為代表的水稻抗毒素及其類似物的結構與活性、黃烷酮類植物抗毒素合成方法的研究概況進行了綜述
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again

    應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。
  7. The appraisal takes the ecology material, the environment material, chemistry material, the toxicology material as the foundation, through the project analysis, the source strong analysis sets a target the pollutant, distinguishes its hazardous nature, the probability, the degree, the scope which the computation risk occurs and so on, the choice appraisal end point, the use appraisal model forecast goal pollutant exposed density, the analysis risk source to the acceptor the harm degree, carries on the risk attribute

    評價以生態資料、環境資料、化學資料、毒理學資料為基礎，通過工程分析、源強分析，確定目標污染物，鑒別其危害性，計算風險發生的概率、程度、范圍等，選擇評價終點，利用評價模型預測目標污染物的暴露濃度，分析風險源對受體的危害程度，進行風險表徵。
  8. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  9. Chinese title, english title, author, the affiliation and address of the first author, journal title, publication year, volume, issue, page, publication type, check tag, history of medicine, grant type, subject headings, classification code, language, chinese abstract, english abstract, animal variety, dosage forms, pharmacology of chinese herbs, pharmacology, name of disease, diagnosis standard, pathogenesis, trial type, treatment of disease, name of drug and formula, chemical name of medicines, english name of medicines, ingredients and dosage of formula, drug compatibility, usage and dosage, acupuncture and tuina points, acupuncture and tuina manipulation, side effect, therapeutic effect guideline, result of cure, materia medica, chemical structure, physical and chemical properies, effective component, chinese medicine identification, method of processing, pharmaceutical techniques, medicinal action and pharmacological effect, pharmacokinetics, and toxicology

    中文文題、英文文題、作者、第一作者單位、第一作者所在地、期刊名稱、出版年、卷、期、頁碼、文獻類型、特徵詞、醫學史、資助類型、主題詞、關鍵詞、分類號、語種、中文文摘、英文文摘、動物品種、劑型、中藥藥理、西藥藥理、疾病名稱、診斷標準、疾病機理、試驗方法、疾病治療、藥名方名、化學藥名、英文藥名、組成劑量、藥物配伍、用法用量、針推穴位、針推方法、不良反應、療效指標、療效結果、藥材學、化學結構、理化性質、有效成分、中藥鑒定、炮製方法、制藥工藝、藥物作用與藥理效用、藥代動力學、毒理學。
  10. This study provides the basis evidence and material basis for prv identification molecular epdiemiology investigation, research of diagnostic reagent and genetic engineering vaccine

    本研究為國內prv毒株鑒定、分子流行病學調查、分子診斷試劑的開發以及基因工程疫苗的研製提供了理論依據和物質基礎。
  11. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  12. Compiled regulations for report of toxicological analysis

    毒物分析鑒定書編寫規程
  13. The two isolates were positive reaction hi polymerase chain reactions with two pair of primers specific for alv - j and gave antigenically strong reaction hi the indirect fluorescence antibody assay ( ifa ) with alv - j specific monoclonal antibody je9. negatively - stained electron microscopic and immune - electron microscopic observation demonstrated that viral particles of the inner mongolia and shandong isolate of alv - j, respectively designated imc10200 and sdc2000 strain of alv - j, showed characteristic morphology of alv

    利用pcr和間接免疫熒光反應進行鑒定，兩株j亞群禽白血病病毒可以被兩對alv - j特異性引物擴增（特異條帶約2 . 2kb和545bp ） ，且在特異性單克隆抗體je9的間接免疫熒光檢測中呈現強陽性熒光反應。
  14. The biological identification of maize dwarf mosaic virus

    玉米矮花葉病毒原的生物學鑒定
  15. Articles published more than 60, 15 excellent article prizes ; 4 translations ; 2 reviews ; 5 works co - ed : 4 prizes, methodology on pharmacological research for traditional chinese medicine associate - editor, won the nationl library prize, the first class prize of library for excellent science and technology in the whole nation, and the third class prize for progress in science and technology in the nation ; methodology on pharmacological experiment, won the frist class preze of library for excellent science and technology in the whole nation ; pharmaclolgy and clinical applications on chinese traditional patent famous recipe associate - editor, and english - chinese and chinese - english dictionary on traditional chinese pharmacology standing editor

    代表性的成果有"兔腦磷脂抗炎免疫藥理研究"為山東省自然基金項目首位，鑒定達國際先進水平，論文發表在中國藥理學通報和中國生化藥物雜志： "磷脂和葛根素防治心血管病實驗研究"為山東省教委課題首位，鑒定達國際先進水平， 2000年獲省教委應用技術成果二等獎"藥物體外吸收實驗裝置的改進和應用" 2000年獲省級優秀實驗技術成果二等獎首位"蝎毒心血管藥理研究" 1991年獲省科技進步三等獎二位。
  16. Amplified in e. coli xi - blue, the eluted phage in the third rounds was poured onto lb / iptg / xgal plate. we selected randomly 18 clones and amplified them, then confirmed positive clones by elisa

    經雙抗體（流感病毒的多抗和辣根過氧化物酶標記m13噬菌體抗體）夾心elisa鑒定的陽性克隆有12個，分別將其純化、並進行dna序列分析。
  17. Identification of alphavirus m1 by molecular method

    一株鷺山病毒的分子生物學鑒定
  18. In the experiment, tick - borne encephalitis virus ( tbev ) mdj - 01 strain isolated in mudanjing in 2001 was identified. and the biological characters and molecular biological characters were studied on, with senzhang strain isolated in 1953 as a control

    本試驗對2001年自牡丹江分離的森林腦炎病毒（ tbev ） mdj - 01株進行了鑒定以及生物學性狀和核苷酸全序列的測定的研究。整個試驗以1953年分離的tbev森張株為對照。
  19. These results suggest that sces are very sensitive to al toxicity hi the root tips of barley and can be also served as a sensitive indicator in al phytotoxicity

    這些結果表明，大麥根尖sce對鋁毒反應極為敏感，可作為植物鋁毒研究的敏感指標。用於鑒定大麥耐鋁性。
  20. Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province. methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province. different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers

    方法：從桂北五步蛇毒腺中抽提總rna ，利用不同的引物，採用一步法（ rt - pcr和pcr在同一管內進行）擴增出不同的dna條帶，利用平端連接的方法將pcr擴增產物克隆至pgem - teasy載體，轉化大腸桿菌jm109 ，挑選白色菌落提取質粒，用pcr對其進行鑒定，直接利用純化pcr產物或提取陽性菌落質粒進行測序。
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