毒酶 的英文怎麼說
中文拼音 [dú]
毒酶
英文
toxenzyme-
A study on using anbijie multienzyme abluent to clear bacterial endotoxin in apparatus for bone marrow collecting
安必潔多酶清洗劑清除采髓器械細菌內毒素的研究Recently, the chinese plant protection agencies reported the growing hardships in controlling some field populations of oriental migratory locust with organophosphate ( op ) compounds. up to now there are more than 600 arthropod species with documented resistance to one or more insecticides and / or acaricides since resistance phenomenon was first described in san jose scale to lime - sulfur in washington in 1908. enhanced metabolic detoxification and reduced sensitivity of insecticide target - sites are the two major mechanisms in resistance development, involving three primary metabolic enzymes of esterases, glutathione s - transferases and cytochrome p450 polysubstrate monooxygenases
代謝抗性是指對殺蟲劑起水解或隔離作用的酶發生改變,從而對殺蟲劑起到解毒作用,昆蟲主要解毒酶系有酯酶、谷胱甘肽- s -轉移酶、細胞色素p450單加氧酶等,這三種酶系任何一種的組成部分發生改變均會引起害蟲抗性的改變;靶標抗性是指由於殺蟲劑作用靶標敏感度降低而產生的抗性。Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。In this experiment, seedlings of arabidopsis thaliana ( col ) were observed after being treated by verlicillium dahliae ( vd - toxin ), exogenous salicylic acid ( sa ), nitric oxide donor ( snp ) and nitric oxide synthase inhibitor ( nna ), then we investigated the changes of endogenous h2o2 content, the activity of the antioxidant enzymes catalase ( cat, ec : 1. 11. 1. 6 ) and ascorbate peroxidase ( apx, ec : 1. 11. 1. 11 ) and mrna levels of cat3 in different stress conditions, we also identified the localizations of h2o2 and no accumulated in the leaves of arabidopsis
本實驗研究了棉花黃萎病菌?大麗輪枝菌毒素( vd - toxin )與擬南芥幼苗互作反應中外源sa 、 no供體snp 、 no合酶抑制劑nna等不同處理對擬南芥幼苗h _ 2o _ 2含量、 cat和apx活性及cat基因mrna表達量的影響,並對no 、 h _ 2o _ 2的積累部位進行染色檢測。Through expert s analysis, aloe contains rich natural protein, vitamin, chlorophyl and the neccessary microelements. it has effects of laxative, stomach care, detoxifcation, detumescence, acesodyne and diminish inflammation. so aloe is usually used to treat astriction, cold, cough, headach, car sickness, bronchia, gastric ulcer, liver disease, hypertension, diabetes, eczema, fleck, chilblain, scald, cancer, etc
經科學分析,它含有大量天然蛋白質、維生素、葉綠素、洛性酶和人體必需的微量元素及蘆蔡大黃素等七十多種成份,具有催瀉、健胃、通經、解毒、消腫止痛、清熱抗炎等作用,對便秘、感冒、頭痛、咳嗽、暈車、支氣管、胃瘍病、小兒厭食癥、肝病、出血癥、高血壓、糖尿病、濕疹、雀斑、凍瘡、燙傷、刀傷、癌癥等數十種疾病有療效。Some viruses contain lipids, carbohydrates and special enzymes.
某些病毒還含有脂類、碳水化合物和酶類。Change of catalase activity in interaction of tobacco and cucumber mosaic virus
煙草與黃瓜花葉病毒互作中過氧化氫酶活性的變化In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,經37 、 1小時處理的病毒,仍然能夠在貓源細胞fcwf細胞上生長,並且毒力基本保持不變;耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0經37作用2小時,毒力僅下降一個滴度;耐熱性試驗中,該病毒在恆定溫度50 ,設定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恆定時間1小時,設定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細胞上完全喪失增殖能力, cpe消失。生物學試驗,利用實驗室現有條件,選擇不同的細胞系對該病毒進行培養,發現該病毒對貓源細胞fcwf最敏感; mdck細胞次之; f81細胞經多次傳代,亦可出現cpe ;而vero細胞則不敏感。血凝試驗表明,該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。Clinical significance of monitoring of cholinesterase in patients with organophosphorus
膽堿酯酶監測在搶救有機磷農藥中毒中的臨床意義The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out
結果顯示, f片段長度為728bp ,與現有生物數據庫的blast比較分析,發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性,推測為一新基因; b片段長約4kb ,序列拼接結果推測靠近tn5插入位點部位有重復序列,對b片段tn5遠端的部分序列進行blast比較,發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。On cytomembrane ; resolved the precipitation in buffer d ( contained triton x - 100 ) shattered by ultrasound, 4 vibrated for 60min, centrifuged at 45000g for 1 hour, took the supernatent to mesure protein concentration and then mesure the pkc activity
目前,在鉛的神經毒性研究過程中,鉛對pkc的作用引起人們極大關注。這是因為鉛比鈣對pkc有更高的親和力。現已知, ph卜可以取代caz 」激活蛋白激酶c 。The monophagous species must produce large quantities of an enzyme to detoxify their food.
單食性種類必須產生大量單一的酶使它們的食物解毒。It launches a team of detoxifying enzymes
身體釋放出一組解毒酶。Beta - exotoxin is an inhibitor of rna polymerase and acts competitively with atp in various biological processes
外毒素是rna聚合酶的抑制劑,在各個生化過程中與atp起競爭作用。Abstract : numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
文摘:許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物,對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明,蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用,也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中,凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料Numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物,對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明,蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用,也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中,凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Effect of enzyme cleaning and eow on complete immersion disinfection of gastroscope
酶潔液和氧化電位水在胃鏡全浸泡式消毒中的效果觀察Effect of dachengqi tang on zymology of oral acute organophosphorus pesticide poisoning
大承氣湯對口服急性有機磷中毒酶學影響的臨床觀察The main bioactivities include antibacterial, antimicrobial, antitumor, antiviral activity, enzyme and enzyme inhibition, and antitumor activity is more important
其生物活性包括:抗菌、抗腫瘤、抗微生物、抗病毒、酶及酶的抑制活性等,其中以抗腫瘤活性最為重要。分享友人