比切克 的英文怎麼說
中文拼音 [bǐqiēkè]
比切克
英文
bycek-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。While the president ' s gift list had a distinctly outdoorsy theme, vice president dick cheney received more artsy presents such as paintings and a statue, according to annual financial disclosure documents released on friday
上周五公布的年度財務調查文件顯示,布希總統收到的禮物大多與戶外活動有關,與之相比,副總統迪克?切尼收到的禮物則更富有藝術氣息,如油畫和雕塑。If the enemy broke through to agedabia, benghazi and everything west of tobruk were imperilled.
如果敵人突破阿傑達比亞,那麼班加西以及托卜魯克以西的一切據點便都岌岌可危了。Leicester cue ace mark selby has had to shelve practice sessions with new partner james wattana after the duo were paired together in the opening round of the welsh open which starts tomorrow
萊切斯特球星馬克?塞爾比和新搭檔詹姆斯?瓦塔納的訓練計劃不得不暫時擱淺,因為他們在明天開始的威爾士公開賽首輪即將相遇。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The blackpool manager simon grayson will fine any player who mentions chelsea as his team prepare to meet norwich city for the right to face the league champions in the fa cup fifth round
布萊克普爾隊主帥西蒙.格雷森宣布在與諾維奇隊比賽前誰到不準提起切爾西,否則將面臨罰款.這場比賽將決定誰能在足總杯第五輪中面對英超的衛冕冠軍Meanwhile, hern n crespo, on loan from chelsea to inter milan, believes injury will force him to miss the rest of the tournament
與此同時,從切爾西租往國米的克雷斯波,相信傷病將使他缺席本屆杯賽的剩餘的比賽。Sportscaster 1 mike winchell to don billingsley will go right
邁克溫切爾傳給跑向右路的唐比靈利[ sportscaster # 1 ] mike winchell to don billingsley will go right
邁克*溫切爾傳給跑向右路的唐*比靈利Whilst delighted at seeing record - signing andriy shevchenko finally open his stamford bridge goal account, jos mourinho was bemused at the bookings handed out to both chelsea goalscorers by referee mark clattenburg
舍瓦終于在斯坦福橋打破他的進球荒,穆里尼奧固然很高興,但是令他吃驚的是本場比賽的主裁判馬克*克拉滕博格黃牌警告了切爾西的2位進球功臣。Ballack limped off at st james ' park following a crunching tackle from titus bramble and, after being assessed by chelsea ' s medical team, was sent to see surgeon hans muller - wohlfahrt in germany
巴拉克再聖詹姆斯公園的比賽中被布蘭布爾鏟傷,在經過切爾西隊醫的診斷之後,他被送往德國外科醫師漢斯穆勒沃爾法赫特那裡做進一步治療。Add 1 pound per barrel check - loss to the active system and drill ahead
按每桶鉆井液加1磅切克勞斯的比例,加一個循環周,然後鉆進。As gm mitch kupchak put it, " it ' ll be about winning and losing, which is the way it should be.
作為球隊經理的庫普切克說, 「那就是關于比賽的勝負情況?它本來應該的方式。 」Chelsea midfielder john obi mikel is in the middle of a row with nigeria coach austin eguavoen
切爾西的中場小將約翰?奧比?米克爾惹惱了奈及利亞隊主教練奧斯丁?伊加沃恩。Chelsea football club can confirm that on tuesday a work permit was granted for jon obi mikel
切爾西足球俱樂部允許喬奧比米克爾得到工作證。“ i ' m letting mitch handle that stuff, ” kobe bryant finally said
「我讓庫普切克來處理這些事, 」科比最後談到。When she said that tomas wasn ' t there and she didn ' t know when he ' d be back, the woman on the other end of the line started laughing and, without saying good - bye, hung up.
在絕望時刻,她總是提醒自己要為他而堅持下去,因為他比她更脆弱,甚至也許比杜布切克和他們離開的祖國還要脆弱。Allow the pill to soak on bottom for 4 ~ 6 hours. add and maintain 2 pounds per barrel of check - loss to the active circulating system
要讓這種堵漏劑在井底滲透4 ~ 6小時,然後在循環系統中,再按每桶鉆井液加兩磅切克勞斯的比例加完,以便維護堵漏性能。No, mitch kupchak didn ' t trade bryant
沒有,庫普切克沒有交換科比。Buss believes in general manager mitch kupchak as much as he believes in bryant
巴斯相信庫普切克猶如他相信科比。分享友人