氨基水解酶 的英文怎麼說
中文拼音 [ānjīshuǐjiě]
氨基水解酶
英文
amidohydrolase-
Cloning and expression of a new acyl - homoserine lactone hydrolase gene
一種新的乙酰高絲氨酸內酯水解酶基因的克隆和表達Several aminopeptidases complete the hydrolysis of peptides into amino acids.
幾種氨肽酶把肽水解成氨基酸。Proteolysis the hydrolysis of proteins into their amino acid. enzymes that catalyze this are proteases or proteolytic enzymes
蛋白質水解：指蛋白質被水解為氨基酸。催化的酶為蛋白酶或蛋白水解酶。4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。L - tryptophan is a neutral, genetically coded amino acid. iit is essential in human nutrition. formed from proteins during the digestive process by the action of proteolytic enzymes
色氨酸是一種重要的中性必需氨基酸，在消化過程中，通過蛋白水解作用酶的運動而由蛋白質形成。Proteolytic enzymes catalyze oligomerization of the. alpha. - hydroxy carboxylic acid and. alpha. - amino acid
蛋白水解酶催化-羥基羧酸和-氨基酸的低聚化。Protease ( proteinase ) an enzyme that catalyzes the hydrolysis of peptide bonds in proteins to produce peptide chains and amino acids
蛋白酶：指催化蛋白質中肽鍵水解為多肽鏈和氨基酸的酶。Hydantoinases which consist of hydantoin hydrolase, n - carbamoylase and sometimes hydantoin racemase can hydrolyze d, l - 5 - substituted hydantoin into corresponding optically active amino acids. therefore, the hydantoinases from micro - organisms are widely applied to the industrial production of natural and unnatural amino acids. microbial hydantoinases are considered as industrial enzymes from the origin of life
乙內酰脲酶是廣泛存在於動物、植物和微生物的一類酶系，包括乙內酰脲水解酶（ hydantoinhydrolase ， hyuh ）和n -氨甲酰基氨基酸水解酶（ n - carbamoylase ， hyuc ） ，有些情況下還包括乙內酰脲消旋酶（ hydantoinracemase ， hyur ） 。The l - n - carbamoylase of arthrobacter bt801, coded by the hyuc gene, is the rate - limiting and the only stereoselective enzyme. hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and, at the same time, activity expression was obtained in e. coli and pichia pastor is gs115 respectively
節桿菌bt801的乙內酰脲水解酶催化乙內酰脲類化合物水解開環， n -氨甲酰基氨基酸水解酶是該菌乙內酰脲酶系中惟一具有立體選擇性的酶，也是整個反應體系的限速酶。To improve the production and activity of the enzyme, the hydantoin hydrolase gene ( hyuh ) was cloned into e. coli m15 and bl21 ( de3 ) by using vector pqe60 and pt221 respectively. two expression plasmids were thus constructed
用pcr技術從質粒puc18 - 169中擴增出l -乙內酰脲水解酶基因（ hyuh ）和n -氨甲酰基氨基酸水解酶基因（ hyuc ） ，分別在大腸桿菌和畢赤酵母中實現了活性表達。As far as the enzymatic activity is concerned, on the one hand, strain yz - ii6 has higher d - hydantoinase activity and lower d - carbamoylase activity so as not to be suitable for one - step bioconversion of d - amino acids, on the other hand, the higher hydantoinase activity, engineered strain in particular, may be convenient to be as a biocatalyst to produce n - carbamyl d - amino acids which hard to find in the markets
Yz - 6菌具有較高的海因酶活性，但n -氨甲酰基d -氨基酸酰氨水解酶活性很低，仍不適合用於d -型氨基酸生產工藝中的一步法轉化。另一方面，含海因酶基因的人工菌株不但酶活性高，也排除了天然菌株轉化生產d -型氨基酸過程中的一些副產物。分享友人