消化酶原 的英文怎麼說
中文拼音 [xiāohuàyuán]
消化酶原
英文
digestive zymogen-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The main study in this paper included as follows : the content and distribution of heavy metals in sediments and benthic organisms from the sewage stream in guangzhou city ; the acute toxicity and joint toxicity of mercury and selenium to swordtail fish ( xiphophorus helleri ) ; the damage of mercury to the indexes of antioxidant system in the gills and livers in swordtail ( including the measurement of the activities of total antioxidative capacity [ t ~ aoc ], superoxide dismutase [ sod ], glutathione peroxidase [ gsh - px ] and the concentration of malondiald - ehyde [ mda ] ) and the relief effects of selenium on it, as well as the physiological damage of mercury on the tissues, namely : the antagonistic effect of na + - k + ~ atpase activity on the tissues between selenite and mercury, and the ultrastructural damage under the exposure of mercury
研究內容主要有：廣州市河涌沉積物及底棲生物體內重金屬含量及評價；汞和硒對劍尾魚的急性毒性和聯合毒性及安全濃度的評價；汞對劍尾魚鰓和肝臟中抗氧化系統的毒性，包括對總抗氧化能力、超氧化物歧化酶、谷胱甘肽過氧化物酶活力及丙二醛含量的測定及硒對其保護作用；汞對劍尾魚組織生理毒性即：汞對na ~ + - k ~ + atpase活力的影響及硒的保護作用和汞和對劍尾魚組織超微結構的損傷等。以高氯酸?硝酸消化法和火焰原子吸收分光光度法測定了廣州市河涌沉積物和底棲生物中重金屬含量。Soya peptone is an enzyme digest of soya, this product contains carbohydrate, widely used in culture media and often used for the cultvation of many fastidious organisms and where rapid, luxuriant growth is required
性狀：本品系由大豆為原料，經酶消化而成，富含碳水化合物，廣泛應用於微生物培養基的配製並常用於營養要求高的微生物培養及滿足微生物快速大量生長的需要。In the stomach, this acid functions to kill bacteria in foods, to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin, to begin the digestion of protein
在胃裡，這種酸的作用是殺司食品的細菌，使食物軟化，把鈍化的胃蛋白酶原轉化為有活性的能消化蛋白質的胃蛋白酶。In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks
方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。The organization cuts into slices and examines by the in situ pcr, drip protease k 20 ( xl with loomg / ml to digest respectively in pretreatment, increase with normal position positive cell account for total ratio of cell, according to the positive standard cells > 75 %, confirm the lightest digestion time, studying the influence and relationship of different fixation time with protease digesting each other, detecting the mn genotype of the organize slices at the same time
石蠟切片進行原位pcr檢，預處理分別滴加loom歲血的蛋白酶k20閃消化，以原位擴增顯色后陽性細胞占總細胞的比值> 75 %為標準，確定最適消化時間『 ， ，研究不同固定時間與蛋白酶消化的相互影響和關系，同時檢測石蠟切片的mn基因型。The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells
組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
應用胰酶消化法進行頜下腺細胞的分離、純化及原代培養、傳代培養。Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin
對照組培養液中不含g廿一1 （ 7一36 ） nhz ，實驗組培養液中含有20nmol / lglp一1 （ 7一36 ） nhz ，培養24h后，用0 . 25 %胰蛋白酶消化胰島分散細胞，塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交， dab顯色，高清晰度病理圖文分析系統（ highpathologiealimageanalysissystem ， hp認s ）對細胞著色的平均光密度（ mean即tiealdensity ， mod ）量化分析，觀察實驗組和對照組胰島素mrna的表達情況。Culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum
含胎牛血清膠原酶消化法培養兔成骨細胞Conclusion the enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes
結論：酶消化法原代培養牛視網膜周細胞是一種穩定、可靠的方法。Methods using different techniques of collagenase digestion and discontinued density gradient centrifugation, the adult swine and rat islets were prepared
方法採用不同的膠原酶消化法及不連續密度梯度純化法。This research use high sensitive, special in situ pcr technology to determine the material which is treated by the paraffin wax and formalin fixation of different time, by detecting the genotype of mn blood group of the organizes slices. at the same time, we study the research of main influence factors, such as protease digestion, etc. we hope to set up a kind of steady, practical methods to detect the genotype of the material treated by paraffin wax and formlin, offer a kind of new detecting means for the forensic appraises and iditificition with individual material evidence
本研究應用靈敏度高、特異性好的原位pcr技術，測定福爾馬林固定不同時間的石蠟切片組織mn血型的基因型，並對蛋白酶消化時間等主要影響因素進行了初步的研究，以建立一種穩定、實用的檢測石蠟組織切片dna遺傳標記的方法，為法醫物證鑒定和個人識別提供一種新的檢測手段。The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition
採用膠原酶消化、重力沉降及密度梯度離心方法分離人胎肝幹細胞，通過免疫細胞化學方法對其進行初步鑒定，以及應用流式細胞儀等對其生長狀況進行評估。In the current study, we tried to isolate liver progenitor cells from retrorsine - treated mouse. after long - term culture and purification, the pure epithelial cell population was established in cobblestone fashion with high nuclear - to - cytoplasm ratios
採用兩步膠原酶消化法從損傷后的再生肝中分離細胞，經過長期的培養和不斷的純化，最終在體外建立了形態均一的連續細胞系。The distribution and accumulation of three kinds of heavy metals including lead, copper and mercury in tissues and cells of several organs, and their effects on activity of digestive enzymes and alkaline phosphatase of macrobrachium nipponense were studied with light microscopy, transmission electron microscopy, cytochemical method, atom absorption spectrum, mass spectrum analysis and enzyme analytical method
本文應用組織學、組織化學、透射電鏡、原子吸收光譜分析和酶學分析等方法，研究了鉛、銅、汞等三種重金屬在日本沼蝦各主要器官細胞內的分佈和積累以及對日本沼蝦消化酶和堿性磷酸酶活性的影響。2. the primary culture of silkworm embryonic cells ( 1 ) this dissertation took silkworm blastokinesis embryo as the materials of primary culture and found a better method of fragment embryo to primary culture by comparing the methods of fragment embryo that mincing embryo by scissors and digest embryo by trypsin after mincinging. that is the latter
2家蠶胚胎細胞的原代培養門）本研究以家蠶反轉期胚胎為材料，採用組織塊法和胰蛋白酶半消化法進行家蠶胚胎細胞的原代培養，建立了適應反轉期胚胎細胞原代培養的方法，即胰蛋白酶半消化法。Protoplast the protoplasm and plasma membrane of a cell after removal of the cell wall, where present. this can be achieved by physical means or by enzymic digestion
原生質體：指去掉細胞壁以後的細胞原生質以及質膜。可用物理方法或酶消化得到。A major obstacle to successful human islet isolation has been the variability of the collagenase digestion phase of islet isolation
成功分離成人胰島細胞的主要障礙是胰島細胞分離中膠原酶消化相的變異。分享友人