混合酶法 的英文怎麼說

中文拼音 [húngěfǎ]
混合酶法 英文
mixed enzyme method
  • 混 : 混形容詞1. （渾濁） muddy; turbid2. （糊塗; 不明事理） foolish; stupid
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 混合 : （攙雜在一起） mix; blend; mingle; admix; mixture; mix up; interfusion; commixture; blending; cre...
  1. We treat the porcine skin by 0. 25 percent trypsin, 0. 125 % trypsin, 2. 5 u / ml dispase, hypertonic saline or hypertonic saline - trypsin / dispase. we find that after the skin has been incubated in 0. 125 percent trypsin for 24h at 4 ?, the cells in the skin are all disintegrated. there are no significant differentiation between the acellular matrix treated by 0. 125, 0. 25 perlent trypsin, 2. 5 u / ml dispase and hypertonic saline - trypsin / dispase. but the cell ca n ' t be removed by using the hypertonic saline - sds

    本研究通過對0 25胰酶不同脫細胞時間處理、不同濃度胰酶處理、 dispase脫細胞法、 im 、 zm高滲鹽水脫細胞法、高滲鹽水和胰酶或dispase混合脫細胞法的比較確認採用0 12盼胰酶， 4 ， 244 。
  2. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  3. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  4. The fungus age, enzyme system, osmotic stabilizer, ca2 + concentration influenced on the formation of the protoplast have been confirmed. the result show that 16 - 18hr fermention, 32 1. 0 % cellulase plus 1. 0 % snailase confected by pba ( phosphate blend ammonium chloride ) contant 0. 2 % ca2 +, acted 3 hr is optimal for the protoplast production of aspergillus. niger

    分別採用單因子法和正交實驗法考查，認為16 18hr菌齡的菌絲體， 32 ，用含0 . 2 ca ~ （ 2 + ）的pba高滲緩沖液配製的1纖維素酶與1蝸牛酶的混合酶酶解3hr ，得到的原生質體數最多。
  5. Dried milk, dried ice - mixes and processed cheese - determination of lactose content - enzymatic method utilizing the glucose moiety of the lactose

    奶粉乾冰混合物和加工的乳酪.乳糖含量的測定.利用乳糖中葡萄糖含量的酶催化方法
  6. The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively

    三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
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