清掃口 的英文怎麼說
中文拼音 [qīngsǎokǒu]
清掃口
英文
clean out-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。We that one a of specialities produce little electrical home appliances the enterprises of productses, locate in and enjoy " mould of china city ", the yuyao of zhejiang of titles of " plastic of china city " ; from 60 kilometers of ports in ningbo, exports 5 kilometers from high speed yuyao of turtledove of hangzhou of shanghai, geographical environment is superior, complete auxiliary facility, produce electric sweeper, electric toothbrush, steam cleaner, car refrigerator, popcorn maker, etc
我司地處享有「中國塑料城」 「中國模具城」之稱的浙江餘姚，距寧波港口60km ，離滬杭甬高速餘姚出口5km ，地理環境優越，配套設施完備。是一家集研發生產出口於一體的小家電系列產品的專業廠家，主要生產電動掃地機電動牙刷蒸汽清潔器爆米花機汽車冰箱等，產品通過tuv gs ce認證，質量第一，交貨及時，竭誠歡迎國內外客商與我們聯系，共同建立業務合作關系。Floor drains with clean - out ; particulars
有清掃口的樓面排水.詳細資料Floor drains with clean - out ; summary
有清掃口的樓面排水.概要說明They also conducted other meaningful activities, such as monthly clean - ups of the community and other environmental improvement projects, community environmental volunteer service work, the start - up of a monthly community magazine, and social dinners, in order to establish a consensus among the people on the value of environmentalism. with support from the local trash collection team, the township office and local residents, the green vanguards converted a litter - strewn wasteland at a major intersection into a beautiful community garden
此外，又進行了多項深具意義的活動：發起每月社區大掃除活動，並擔任社區環保志工，協助打掃整理環境協助發行社區月刊及舉辦社區聯誼聚餐活動，以凝聚共識在清潔隊鎮公所和鄉親的支持下，將社區路口原本一處臟亂的荒地，美化成一個漂亮的花園。The road sweeping cleaning, enhancing sweeping frequency, asking for " seven - eight net, " that is no deposit, no litter ( pack ), the peel of debris - brick, sand, mud without water, without tanji stains, ground - graffiti was chaos posted, the road net, net wellhead groove, lower part of wall net, net - poles, tree point net, net roadside stone teeth, green belts, net, net garbage bin
在道路清掃保潔方面，提高清掃頻率，要求達到「七無八凈」 ，即無堆積物、無垃圾（包） 、無果皮紙屑、無磚瓦土石、無污泥積水、無痰跡污漬、地面無亂塗寫亂張貼，路面凈、溝井口凈、墻根凈、電桿根凈、樹穴凈、路邊石牙凈、綠化帶凈、果皮箱凈。Gatt was designed to lower import taxes and remove other barriers to trade in goods
關貿旨在降低進口稅及掃清商品貿易障礙。In this paper, a lot of macro and micro fracture sections under equal and variable amplitude loads are analyzed on 40cr high - strength bolts by use of scan - electronic microscope. its fatigue mechanism is known fundamentally
本文以螺栓球節點網架中最常用的40cr高強螺栓為研究對象，藉助掃描電子顯微鏡等先進儀器對40個常幅及變幅疲勞試件斷口進行了宏觀及微觀形貌分析，基本摸清了其疲勞破壞的機理。分享友人