清選篩 的英文怎麼說

中文拼音 [qīngxuǎnshāi]
清選篩 英文
cleaning screen
  • 清 : Ⅰ形容詞1 （純凈） unmixed; clear 2 （寂靜） quiet 3 （清楚） distinct; clarified 4 （一點不留） w...
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 篩 : 名詞[書面語] （植物名） sedge
  1. Experiment shows that the antigen extracted by autoclave is best for dot - ppa - elisa. applying the x2 test, optimal concentration of antigen was determined, and the optimum dilution of enzyme hrp - spa was 1 : 10. the high specificity of dot - ppa - elisa was proved by the specific blocking test, and also by the cross - reaction test in which the diaphragm does not react with the antibodies against salmonellosis, pasteurellosis, chlamydiosis, hcv, ppv, brucellosis, erysipelas suis, colibacillosis, prv

    試驗中對多種方法提取的診斷抗原進行了比較、篩選；確定了dot - ppa - elisa的最佳工作條件；測定了st - 171株的免疫豬血清抗體及c群、 2型人工感染豬血清抗體效價，並初步確定了該方法的陽性標準。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。
  4. Technique specification for fine air screen seed cleaner

    風篩式種子清選機.技術條件
  5. On a basis of the developmental inspirations acquired from disputes of “ canon ” for the past half century, this text intends to review the influence of traditional poems collection through procedures of sifting basis and canonization, in hopes of discovering poems collection to be actually the most concrete and effective canon style among traditional poetics ; three types of poetic canons are also concluded within a range of poems collection in ming and ching dynasty

    摘要本文欲藉助近半世紀典律論爭獲得的發展啟示，由篩選基準、正典化等過程上，重新檢視傳統詩選集的影響力，發現詩選集確實是傳統詩文論中最具體而有效力的典律形式；也以明清詩選集為范圍，歸結出三種詩典律的類型。
  6. Improvement of stalk epidermis flap shaker and design of cleaning device for stem sieve

    煙拐剔除篩的改進及煙梗分選篩清理裝置的設計
  7. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  8. The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before

    重組菌用甲醇誘導表達，用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達，對表達上清處理后，用sds - page和western - blot進行鑒定。同時，用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。
  9. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  10. According to the results of antibiotic and chinese herb sensitive experiment to isolate, screened the high sensitivity herb and composed " changjunqing powder ", the clinic control test results showed the effectiveness level was 88. 9 %

    根據抗生素及中藥對分離菌的藥敏試驗結果，篩選出敏感中藥並組方研製出「腸菌清散劑」進行臨床防治試驗，總有效率為88 . 9 % 。
  11. Trio currently offer portable jaw, impactor, cone, screening and washing system that can be shipped economically throughout the world

    Trio公司現行所提供的移動式顎式破碎機、反擊式破碎機、圓錐式破碎機、篩選機及清洗系統均可以最經濟的方式向全世界供貨。
  12. Among 54 arbitrary primers tested, only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae

    本研究經用54種隨機引物對57種蟋蟀的rapd擴增分析得出以下結論： 1 ）採用rapd技術，首次較系統地對中國蟋蟀總科7科26屬共57種蟋蟀進行了系統學研究。在所試用的54種隨機引物中篩選出可擴增出清晰可辨且穩定的rapd圖譜的引物分別為：蟋蟀科9種、嶺蟋科9種、樹蟋科12種、結蟋科12種、蛛蟋科13種、鐵蟋科13種、貌蟋科13種。
  13. Find data in a list by using filters or the lookup wizard

    查找清單中的數據請使用篩選或查詢向導。
  14. If you are uncertain about the exact name or spelling of your point of interest, enter as much as you know ( even if it s only the first letter ) and we will return to you a list of similar points of interest

    若對景點的正確名稱或拼法不確定，盡可能輸入您所知道的（即使只是第一個字母） ，您即可得到與輸入資料接近的清單，然後再篩選。
  15. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  16. Biological activity of this fusion protein was detected by vsv / wish system, the result was 4. 104. 1 104iu / m =

    將篩選出來的重組菌株誘導144小時，取菌液上清液用vsv wish系統檢測重組蛋白的抗病毒活性為4
  17. Water tank tower cleaning firms, the twd has stipulated a set of standardized operating procedures for cleaning and monitoring user - end water quality. to ensure the firms service quality for water tank tower, the twd has also provided training for and reviewed private cleaning firms to select qualified ones for users reference. follow - up inspection and unscheduled examinations are carried out by the twd

    有鑒于坊間水池水塔清洗作業品質與價格良莠不，本處已制定清洗與監控用戶端水質標準化作業，給予清洗業者訓練及審查，篩選出合格業者，推薦給用戶參考，以保障水池水塔清洗品質。
  18. Each package that calls the extraction module can perform different data scrubbing, filtering, or aggregation operations

    調用提取模塊的每個包都可以執行不同的數據清理、篩選或聚合操作。
  19. B. the marble primarily is a dark green and the red. particularly the dark green marble, color is beauty, is called the lingshou green ", supply falls short of demand. in addition, open mines the surplus s broken bits to process marble rice, marble powder

    我單位生?的是天然鵝卵石，取自於河床， ?色主要有灰色青色暗紅三大色系，經過清洗篩選分撿等工序，可以用作鋪設路面公園假山盆景填充材料等。
  20. Testing and qualification method for air - screen separator

    風篩清選機試驗鑒定方法
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