測序引物 的英文怎麼說

中文拼音 [cèxùyǐnwù]
測序引物 英文
sequencing primer
  • 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
  • 引 : Ⅰ動詞1 （牽引; 拉） draw; stretch 2 （引導） lead; guide 3 （離開） leave 4 （伸著） stretch 5 （...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  1. By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1

    首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。
  2. Considering of the specificity of the degenerative primer designed in this pcr reaction, the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa, asmaspb, clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi, a japanese ascidian, we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene

    基於本實驗中所設計的引物為特異性簡並引物，測序基因通過比較得到與預期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事實，我們可以初步推測，本實驗pcr反應所克隆的片段可能為文昌魚masp或其同源基因的一部分序列。
  3. The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector. their cdna sequences were determined with positive clones or purified pcr product. conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province, their homology is 90. 6 %

    結果：其中一對引物擴增得到一600bp產物；另一對引物擴增得到三條特異性的dna條帶，大小分別為1 . 5kb 、 1 . 3kb和800bp ，將600bp和800bpdna進行克隆及測序，並推導編碼的氨基酸序列。
  4. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  5. Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

    二、研究論文1 、參照人sry基因hmg - box保守區的序列，設計一對兼并引物， pcr擴增了中華絨螯蟹的sox基因，並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ，其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ，顯示該基因在進化上具高度的保守性。
  6. In this paper, 45 e. coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e. coli by animal test. type 1 pili of 45 strains isolated was detected by msha. the pila gene of 45 avian pathogenic e. coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank. results showed that pcr was more sensitive, faster and more characteristic than msha to detect type 1 pili

    本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株，採用d -甘露糖敏感血凝試驗（ msha ）檢測1型菌毛，根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。
  7. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  8. Ghrelin regulates pituitary growth hormone ( gh ) secretion. in the study, we obtained the cdna sequences of preproghrelin and the cdna encoding the ghrelin of duck, goose and emu by using rt - pcr and 3 " - race methods. and then deduced the amino acid sequences of preproghrelin and ghrelin in duck. goose and emu. sense and antisense primers were designed on the basis of chicken ghrelin cdna sequence ( dest accession no. ab075215, 836bp ) and proventriculus cdna was performed as the template

    Ab075215 ， 836bp ）設計引物，以鴨、鵝、鴯鶓腺胃cdna為模板，通過rt - pcr及3 - race的方法，將各個產物經過回收，再經連接轉化，克隆測序，拼接后得到鴨、鵝、鴯鶓preproghrelin基因的cdna序列及ghrelin的cdna序列，並由此推測出preproghrelin及ghrelin的氨基酸序列。
  9. In this paper, total dna from tannage, tail skin, scales and the skin treated with salt were successfully extracted with an improved method for dna extraction. to verify the results, four pairs of primers, which were universal primers for 12s rrna gene, diagnostic primers of chinese alligator meat, microsatellite primers and rapd primer, were used to do pcr amplifications. some amplified fragments were sequenced, either

    本文研究出一種改進的dna提取方法，成功地從揚子鱷鞣製皮革中提取了總dna ，同時對不同的組織標本如鱗片、鹽腌生皮及尾尖皮等進行了dna提取：並利用12srrna基因擴增的通用引物、揚子鱷鑒定性引物、微衛星引物及rapd引物進行pcr擴增，且對部分pcr擴增結果進行測序，以檢驗提取效果。
  10. The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods

    根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。
  11. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  12. The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank. concentration of tp was analyzed after amplification invitro by rt - pcr, purified by low melt agarose and labeled by digoxigenin

    根據genbankth - 98株序列設計第2對引物，應用rt - pcr方法體外擴增該片段（命名為tp ） ，瓊脂糖凝膠純化后測定其濃度和純度，進行非放射性地高辛標記。
  13. The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced

    首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域，共設計出7對適合rfq - pcr的引物（依次命名為primer1 （ f r ） primer7 （ f r ） ） ，並用常規pcr實驗初步證明其中有4對引物（ primer1 （ f r ） 、 primer2 （ f r ） 、 primer5 （ f r ） 、 primer6 （ f r ） ）可作為中肋骨條藻特異性引物的候選者；然後測定了以primer6 （ f r ）為引物的pcr產物的序列，序列分析表明，中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大，從中可設計出滿足rfq - pcr需要的taqman探針（命名為taqman6 ） ；進一步的核酸雜交實驗表明， taqman6隻與中肋骨條藻的pcr產物雜交，不與其他藻的pcr產物雜交。
  14. The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software

    方法根據mtdna控制區及其周圍區域的序列，設計多對引物，探索優化擴增體系，使擴增條件能夠同時滿足多對引物的需要，用sanger末端終止法及熒光標記技術對樣本進行dna測序， sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。
  15. The full - length cdna of hbrp is amplified by pcr reaction and sequenced after cloning. 3

    在序列的兩端設計引物， pcr擴增全長cdna ，克隆后測序分析。
  16. 3 characters of its sequences in the cardamine pcr technique was used to amplify its and the product was directly to be sequenced with two ways sequencing primers on sequenator

    3碎米屬植物的ts序列特徵採用pcr擴增，混合擴增產物在自動測序儀上雙向引物直接測序，測定了碎米蕎屬（ ca廠內。
  17. 2. using a pair of degenerate primers based on the conserved region, hmg - box, of human sry gene, eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced

    2 、參照人sox基因設計了一對兼并引物，擴增了虎紋蛙的sox基因，並對擴增產物進行了克隆和測序。
  18. The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity. a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila. with the specific primers, a target fragment about 1. 1kb was amplified from aeromonas hydrophila l316 via pcr. the target fragment was inserted into the linearized pgem - t easy vector

    根據已發表嗜水氣單胞菌的外膜蛋白基因omp的核苷酸序列設計引物，利用pcr技術，擴增、克隆了嗜水氣單胞菌l316的主要外膜蛋白基因（ momp ） ，經t a克隆，插入到pgem - t系列載體上，測序分析結果表明momp基因最長的開放閱讀框（ orf ）為1035nt ，編碼由344個氨基酸組成，分子量為36kda的主要外膜蛋白質（ momp ） 。
  19. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  20. The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos. all of the eight segments were amplified by rt - pcr, and the purified pcr products were done cycle sequencing with specific primers, furthermore, the sequencing products were purified and run gel on abi prism 377 dna sequencing machine. the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4. 0 version ) and genedoc ( 2. 3 version ) software

    首先將實驗用毒株在spf雞胚中增殖，提取核酸，然後應用oligo （ 4 . 0版本）和gendoc （ 2 . 3版本）軟體設計h9n2aiv所有8個基因片段特異的pcr引物及序列測定引物，通過rt - pcr方法擴增所有毒株的各個基因片段，純化後用特異引物進行測序反應，反應產物純化后在abiprism377dna序列分析儀上進行序列測定，然後應用wisconsinsequenceanalysispackage （ gcg ， 10 . 2版本） 、 phylogeneticanalysisusingparsimony （ paup ， 4 . 0版本）和treeview （ 1 . 5版本）軟體進行序列的數據編輯、序列翻譯、進化樹繪制和遺傳演化分析。
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