游高核蛋白體 的英文怎麼說

中文拼音 [yóugāodànbái]
游高核蛋白體 英文
free ribosome
  • : Ⅰ動詞1 (人或動物在水裡行動) swim 2 (各處從容地行走; 閑逛) rove around; wander; travel; tour 3...
  • : Ⅰ形容詞1 (從下向上距離大; 離地面遠) tall; high 2 (在一般標準或平均程度之上; 等級在上的) above...
  • : 核構詞成分。
  • : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
  • : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
  • : 體構詞成分。
  • 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
  1. A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so

    以質粒ppgedna為模板, pcr擴增出1 . 7kb的ge基因完整片段,將擴增產物以ecori和bamhi雙酶切后,插入原表達載pbv220的p _ rp _ l啟動子下的ecori和bamhi位點間,得到重組表達質粒pbvge ,轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后,用sds - page和western - blot ,以及瓊脂雙擴散來檢測,結果表明prvfa株ge基因在原上得到效表達,表達產物約占總的17 。
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