源基子 的英文怎麼說
中文拼音 [yuánjīzi]
源基子
英文
minamoto no motoko-
Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量,我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列,目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上,構建ppic9k - fl表達質粒。Dreb - type transcription factors can accept stress signals from environment and promote the expression of stress - tolerant genes. four dreb homolog genes, gmdreba, gmdrebb, gmdrebc and osdreb4 - 2 were isolated from soybean [ glycine max ( l. ) merr. ]
Dreb類轉錄因子能接受逆境信號並啟動逆境應答基因的表達。在本研究中,以大豆和水稻為材料,共克隆了4個dreb的同源基因,分別命名為gmdreba , gmdrebb , gmdrebc和osdreb4 - 2 。Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反義乙烯受體leetr1 , leetr2基因番茄t _ 0代種子為實驗材料,利用gus基因表達研究外源基因的遺傳規律,並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性,以及靶基因的表達特性,初步探明了它們在乙烯受體系統中的功能。We transferred the hetero - logous gene into toreniafournieri successfully. this method laid a foundation for study of double fertilization of angiosperm by transgenic tecnology
我們將外源基因成功地導入藍豬耳,為利用轉基因的手段研究被子植物受精和胚囊發育打下基礎。The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli
從轉化的平板中篩選出陽性重組子,進行不同iptg濃度和不同誘導時間的表達研究,並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ ( 12 )在大腸桿菌eSome researchers think that for many defectie genes, the best place to make the repair is at the source - - the sperm of the eggs
一些研究人員認為,對許多有缺陷的基因,最好的修復位置就是其根源? ?精子或卵子。The thesis mainly discusses the design and implementation of electronic chart basic plat which is the essential part of ecdis
本文重點研究ecdis的墓本信息源?電子海圖基礎平臺的構建與實現問題。Electroporation of sperm to introduce foreign dna into the genome of pinctada maxima jameson
大珠母貝精子介導外源基因轉移研究Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high
結果表明: ( 1 )脂質體包裹外源基因轉染精子的方法,可將外源基因導入受精卵中,能夠獲得轉基因動物,並得到了較高的轉基因陽性率; ( 2 )精子攜帶的外源dna的整合過程是隨機的,在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。Gene cassette could be integrated into an integron with recombination between atti and attc sites by integrase ( inti ), or excised from an integron and become a free circle molecule
基因盒可從整合子中切除成為游離的環狀分子,或者將外源基因盒捕獲並整合入整合子。Diamond - like carbon gradient film on ti6a14v alloy substrate have been prepared by means of plasma source ion implanted - ion beam enhanced deposition ( psii - ibed ). for potential applications as artificial joint materials and artificial cardiac valve materials, its trobological performance and hemocompatibility has also been evaluated in the present ph. d. thesis
本研究採用等離子源離子注入?離子束增強沉積技術( psii - ibed )制備了鈦合金基類金剛石梯度薄膜材料,對類金剛石梯度薄膜這一新型人工關節材料和人工心臟瓣膜材料的生物摩擦學性能和血液相容性進行了研究和評價,研究了摩擦磨損對材料血液相容性的影響。Thus it ' s necessary to recombine the heterogeneous gene with the promoter of laticifer - expression gene, and then the heterogeneous gene will express in laticifers
為此需將外源基因與乳管表達基因的啟動子連接,令其在乳管中表達。In this paper, the whole process of it microsporogenesis and male gametophytes development was observed with microscope to sure weather stamen development is normal. at the same time, in order to provide techniques on biotechnology conservation and the foundation of its resources gene pool in cell engineering, its techniques on culture in vitro was studied
本論文通過對蝟實小孢子發生和雄配子體發育全過程進行細胞觀察,探尋蝟實的雄性器官的發育是否是蝟實有性生殖的薄弱環節,並對蝟實的離體培養進行了初步的研究,為蝟實生物技術保存、建立蝟實種質資源基因庫提供細胞工程方面的途徑和技術。Cadastral database is an important branch of land and resources databases, and the effective management of cadastral database is a guarantee to the application and sustainable development of cadastral management information system
摘要地籍數據庫是國土資源基礎數據庫中重要的一個子庫,地籍數據庫的有效管理是地籍管理信息系統應用及可持續發展的一個基礎保障。The chimaeric molecule, containing the vector dna and inserted foreign dna, is introduced into bacterial cells where it multiplies.
含有載體的DNA以及插入的外源基因的雜合子,被引入細菌細胞,並且在其中增殖。I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids, to create a gene targeting insertion vector designated gfp tnos. after agro - infiltration into transgenic nicotiana benthamiana 16c, progeny were analyzed genetically for phenotypic changes, sirna accumulation, and dna methylation
採用農桿菌浸潤法( agro - infiltration )感染轉基因本生煙16c ,並對同源基因瞬時表達所引起的植物表型變化、小分子rna的產生、 dna甲基化程度、以及相關性狀在後代中的遺傳情況進行了檢查。The blast analysis of the 3. 0kb segment indicate that it is similar to translational activator and has more than 80 % homology to two genes in arabidopsis and oryza saliva respectively
將所得約2 . 8kb的基因片段作blast分析,表明其可能屬于翻譯激活子基因家族,並在擬南芥和水稻中皆有同源性大於80的同源基因。Children learn about conserving resources and solar energy at the windmill station, and find out about nature and life at the butterfly park and mangrove trees
會在再生能源基地教孩子用太陽能推動模型車,學習珍惜資源。又會帶孩子到彩蝶園紅樹林,認識大自然和生命的奧秘。The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing
豬瘟病毒e2基因的真核表達:分別將csfv兩個代表株的e2基因克隆入畢赤酵母( p . pastoris )分泌型表達載體ppic9k中,酶切線型化后電穿孔導入p . pastotis進行整合,經g418篩選得到25個高拷貝轉化子,經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。The chimaeric molecule, containing the vector dna and inserted foreign dna, is introduced into bacterial cells where it multiplies
含有載體的dna以及插入的外源基因的雜合子,被引入細菌細胞,並且在其中增殖。分享友人