溴化白蛋白 的英文怎麼說
中文拼音 [xiùhuàbáidànbái]
溴化白蛋白
英文
beta-eigon-
Conclusion compared with amine reduction, cyanogen bromide activation was more suitable for the preparation of pneumococcus type 1 conjugate vaccine
結論採用溴化氰活化法制備1型肺炎球菌莢膜多糖蛋白結合疫苗優于胺還原法。A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity
報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑(溴化十二烷基三乙銨/十二烷基硫酸鈉)分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基,研究蛋白質在雙水相體系中的親和分配.結果表明,在該體系中,表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成,不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性The large - scale expression product is concentrated and desalted by ultra - filter, and the target protein is separated by affinity chromatography with chitin, and then cut by cnbr. the purified product appears evident antibacterial activity with method of agar diffusion
採用優化的表達條件進行大量發酵,對上清液超濾濃縮脫鹽后,採用幾丁質基質進行親和層析分離目的蛋白,溴化氰切割,瓊脂孔穴法測抗菌活性。
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