溶胞產物 的英文怎麼說
中文拼音 [róngbāochǎnwù]
溶胞產物
英文
lysate-
The metabolites eliciting inhibition to foam cell formation process of macrophage produced by endophyte hccb00017 were studied. several products were isolated through solvent extraction, and silica gel chromatography et al. one compound, hccb00017 - a, showed cytotoxicity ; the other two, hccb00017 - c and hccb00017 - e, showed inhibitory activity against foam cell formation process of macrophage
對具有巨噬細胞泡沫化抑制活性的植物內生菌hccb00017的代謝產物進行研究,應用溶媒萃取、硅膠柱分離等方法,從其發酵液中分離出具有細胞毒性的活性物質hccb00017 - a ,以及具有巨噬細胞泡沫化抑制活性的組分hccb00017 - c和hccb00017 - e 。Histopathology of epinephelus akaara injected with the extracellular products from vibrio alginolyticus
溶藻弧菌胞外產物注射赤點石斑魚后的組織病理學觀察As well as providing a new tool to combat bacteria now, there is interest in developing bacteriophage lysins to replace antibiotics in some applications in the future
作為對抗這些細菌的新的藥物工具,人們對將來開發噬菌體細胞溶解酶在某些條件下代替抗生素產生了濃厚的興趣。Studies of the crystal structure of endostatin have shown a compact globular fold, with one face particularly rich in arginine residues acting as a heparin - binding epitope, this site was recently shown to be involved in the inhibition of induced angiogenesis. experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion. recombinant endostatin from bacteria is largely insoluble, but still efficient in arresting tu mor growth after injection into mice. intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors, including lewis lung carcinoma, to a dormant state. no sign of drug induced resistance has been reported and, in the original study, the treatment dormancy appeared to persist even when therapy was discontinued. sowe regard endostatin as a promising anti - tumor drug
許多研究表明重組內皮抑素特異性抑制內皮細胞增殖,而且這種抑制作用呈劑量依賴性。細菌表達產物內皮抑素大部分以不溶形式存在,將這種混懸液注射治療老鼠仍可以抑制腫瘤生長。于小鼠皮下重復注射內皮抑素重組蛋白,幾乎完全抑制鼠lewis肺癌等多種腫瘤生長,並無耐藥性產生,即使中斷治療腫瘤也不再復發。It is considered that pretreatment could improve the physical and chemical of ofmsw characteristics, such as solubility, acidity, alkalinity, and biodegradability, and accordingly increase soluble chemical demand ( cod ) and volatile fatty acid ( vfa ), enhancing biogas yield, reducing hydraulic retention time ( hrt ), optimizing anaerobic process and releasing post - treatment
研究認為,通過溶胞處理能夠改善有機垃圾的物理化學性質如發酵物料的溶解度、酸堿度等,提高微生物對難降解有機物的分解,增加可溶性cod和揮發性酸的濃度,優化發酵細菌的代謝途徑以及產物的組成等,從而增加生物氣產量,縮短水力停留時間,強化厭氧發酵過程,減輕了后續處理的負擔。The mature gene of gloshedobin was inserted into the expression vector ppic9k. the yeast pichia pastoris strain gs115 was applied to study the expression
經sds - page ,親和層析及電鏡分析,表達產摘蟄物主要以可溶性的蛋白質形式存在於細胞質中。The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明,融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上,融合蛋白主要以不溶性包涵體的形式存在於細胞中,少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。Research development of the relationship between actinobacillus actinomycetemcomitans and apoptosis in periodontitis
可溶性產物對體外培養的多形核白細胞The originalities of this paper are : 1, development of a a flow cytometry - based assay for quantitative analysis of cellular proliferation and cytotoxicity in vitro ; 2, the soluble secretion of k. 562 cell lines reduce the number of pbmc, but promote the activity of pbmc in dose - dependent manner ; 3, soluble secretion of k562 cell lines can induce the no production by pbmc, but no only plays a part of the role of soluble secretion of k562 cell lines ; 4, establishing a in vitro model and giving some parameters for sreening and appraising anti - tumor medicine
本研究的創新點在於: l 、建立了用流式細胞術定量測定細胞增殖和細胞毒性的直接檢測技術; 2發現k562可溶性分泌物劑量依賴性地使pbmc細胞數量減少但活性增加; 3 、 k562可溶性分泌物能誘導pbmc產生no ,但是no的作用並不等同於腫瘤上清的全部作用。 4 、為抗腫瘤藥物篩選提供了一個體外模型,並明確了一些篩選指標。The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性,通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列,並克隆到轉移載體pbacpak9上,通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內,進行表達。表達產物分泌到上清中,共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ,比未引入連接肽的scfv - uk32的表達活性25 iu ml高。In this paper, the effect of plant cell aggregates, oxygen supply and gas composition, broth rheology, and hydrodynamic stress on plant cell growth and metabolite production in plant cell culture was discussed in detail
本文針對植物細胞培養的基本特點,詳細討論了與大規模生產有關的工程技術方面的問題,如植物細胞聚集、溶氧及氣體成分、流體性能、剪切力對植物細胞培養產生的影響。分享友人