無凝血酶 的英文怎麼說
中文拼音 [wúníngxiě]
無凝血酶
英文
athrombia-
In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。The operation gets used to card to be : is diagnosed clear, estimate yu yixie of pathological changes confine or half liver person ; does not have apparently icteric, ascites or far transferrer ; countervail of liver function generation is still good, thrombin time not under 50 % person ; the person that skill of work of heart, liver, kidney suffers
手術適應證為：診斷明確，估計病變局限於一葉或半肝者；無明顯黃疸、腹水或遠處轉移者；肝功能代償尚好，凝血酶時間不低於50 %者；心、肝、腎功能耐受者。Method to collect respectively 180 unrelated males " venous blood 500ul, who lived in shanxi province, 120 unrelated mongolians " venous blood 500ul, who lived in the inner mongolia autonomous region, and the blood is anticoagulant with edta, then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr
方法採集180例山西漢族和120例內蒙古蒙古族男性無關個體靜脈血各500ul ， edta抗凝，用tkml液反復洗滌至無色，加入2蛋白酶k緩沖液180ul ，蛋白酶k （ 20mg ml ） 20ul ，在56消化至液體清亮為止，用酚-氯仿法抽提dna ， pcr擴增dys413位點， 6非變性聚丙烯酰胺凝膠電泳， 1硝酸銀染色分型。
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