無性敏殖 的英文怎麼說

中文拼音 [wúxìngmǐnzhí]
無性敏殖 英文
molecular cloning
  • 無 : 無Ⅰ動詞（沒有） not have; there is not; be without Ⅱ名詞1 （沒有） nothing; nil 2 （姓氏） a surn...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 敏 : Ⅰ形容詞（靈敏;敏捷） quick; agile; smart; nimble Ⅱ名詞（姓氏） a surname
  • 殖 : 殖Ⅰ動詞（生息; 孳生） breed; grow; multiply Ⅱ名詞（姓氏） a surname
  • 無性 : [生物學] asexual; [植物學] neuter; agamo 無性繁殖 vegetative propagation; 無性生殖 asexual reprod...
  1. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  2. Part i effects of gnt - v on cell proliferation, cell sensitivity to egf and the egf receptor of h7721 cell line using mtt method, it was found that the proliferation of cells transfected with sense gnt - v cdna was facilitated, and both of the total 3h - tdr incorporation and the specific incorporation per cell were also increased. oppositely, these parameters were reduced in cells transfected with antisense cdna of gnt - v. these results suggested that cell proliferation and dna synthesis were modulated by gnt - v

    第一部分gnt - v對h7721細胞生長、 egf敏感性和egf受體的影響用mtt方法發現轉染正義gnt - vcdna的h7721細胞增殖速度加快，而且無論是~ 3h - tdr的總參入量或每個細胞的參入量均見增加，說明dna合成增強，而轉染反義gnt - vcdna的h7721細胞則完全相反， dna合成和細胞增殖速度均見降低，這提示gnt - v可調節細胞的生長。
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