無氧培養 的英文怎麼說
中文拼音 [wúyǎngpéiyǎng]
無氧培養
英文
anaerobic culture-
Results showed that in the water body of xizi lake, annual average of culturable planktonic ammonifiers and nitrogen fixers were 510 and 236 cfu / ml, respectively ; ammonia oxidizers, nitrite oxidizers, nitrate reducers and denitrifiers were 8. 5, 16, 587 and 16 mpn / ml, respectively ; inorganic phosphate solubilizing bacteria ( 1pb ) and organic phosphorus mineralizing bacteria ( opb ) were 89 cfu / ml and 37 mpn / ml, aerobic and anaerobic cellulose decomposers were 7 and 5 mpn / ml, respectively
水體中可培養異養細菌（氨化細菌）和固氮菌的年平均值分別為510和236cfu ml ，氨氧化細菌、亞硝酸氧化細菌、硝酸鹽還原菌和脫氮菌的數量分別為8 . 5 、 16 、 587和16mpn ml ；無機磷和有機磷分解菌分別為89cfu ml和37mpn ml ；好氧性纖維素分解菌和厭氧性纖維素分解菌只有7和5mpn ml 。The main factors that influence the anaerobic granulation process were summarized, including inoculation seeds arid its concentration, nutrients, inorganic ions, temperature, as well as hydraulic conditions
摘要總結了影響厭氧污泥顆粒化進程的幾種主要因素，包括接種污泥的種類和濃度，營養物的種類和濃度、無機離子、培養溫度以及水力條件等。N sources ( including organic and inorganic n tested ) and c sources tested could restrain methane oxidation. cellulose inhibited methane oxidation most weakly while the high concentration of methanol and glucose did dramatically, but the proper concentration of methanol could stimulate soil methane oxidation sharply. in the middle process of methane oxidation, addition of glucose could restrain methane oxidation shortly but the inhibition could be relieved about 5 days later when supplied again with enough oxygen
土壤微生物是甲烷氧化的主要生物類群，含水量對土壤甲烷氧化活性有明顯影響，過高或過低對甲烷氧化均具有抑制作用；氮源（包括有機和無機氮源）對甲烷氧化均有抑制作用；不同碳源對甲烷氧化的影響各異，纖維素對甲烷氧化抑制作用最小，而高濃度的甲醇、葡萄糖則對甲烷氧化具有強烈抑制作用；而適當濃度的甲醇可極大促進土壤對甲烷的氧化：在甲烷氧化過程中加入葡萄糖能迅速抑制甲烷氧化；在加入葡萄糖的同時保持瓶中充足的氧氣，則這種抑制作用可以在重新培養一定時間后得到解除。Had the highest activities of xylanase and cmcase among all isolates. with rice straw as fermentation substrates, the maximum activities of xylanase and cmcase by a4 were 13. 54 u ? ml - 1, 0. 25 u ? ml - 1, respectively
第二部分對培養基底物及主要組分無細胞瘤胃液和酵母膏對厭氧真菌a4菌產酶的影響進行了研究。The reduced level of cell - free rumen fluid had no significant effect on xylanase production, but had significant effect on the cmcase activity. without cell - free rumen fluid, the high concentration level of yeast extract could improve xylanase and cmcase production. in the third section, crude enzymes produced by anaerobic fungus a4 was extracted, and their characteristics of the crude enzyme was also investigated
與基礎產酶培養基相比，降低培養基中無細胞瘤胃液濃度對厭氧真菌所產木聚糖酶的酶活及比活力無顯著影響（ p 0 . 05 ） ，但對其所產的羧甲基纖維素酶的酶活及比活力有顯著影響（ p 0 . 05 ） 。Flexor digitorum profundus tendons of chickens wer e cultured in the presence of deoxyguanosine ( dgua ) for 5 days, then cryopreserv ed in liquid nitrogen ( 196 ) without affecting their viability
用脫氧鳥苷培養處理雞屈趾深肌腱5天，無創凍存於液氮貯存器（ 196 ）中3個月，使用前將凍存腱在40溶液中融解並洗去腱中吸收的二甲基亞碸。In vitro injury models of brain slice ( ogd and nmda insult ) and primary neuronal cultures ( nmda insult ) oxygen / glucose deprivation ( ogd ) - induced injury of rat hippocampal slice in vitro the rat hippocampal slices prepared were allowed to recover in the normal artificial cerebrospinal fluid ( acsf ) bubbled with gas mixture of 95 % o2 + 5 % co2 for 1 h, then they were thansfered to glucose - free nacsf which was bubbled with gas mixture of 95 % n2 + 5 % co2. after treatment with ogd, the slices were placed into 2 % ttc solution in dark and incubated at 37 * cfor 1h. the slices were weighted and a 50 : 50 mixture of ethanol / dimethyl sulfoxide was then added to extract the formazan in dark for 24 h
離體腦片損傷模型（ ogd和nmda ）及原代培養神經元nmda損傷大鼠離體海馬腦片缺氧缺糖（ ogd ）損傷大鼠離體海馬腦片制備后，在通氧混合氣的正常腦脊液（ nacsf ）中恢復60min ，然後移入通氮混合氣的無糖腦脊液（吵化sf ）中缺氧缺糖，取出腦片與2 ttc避光37 』 c溫浴60dll ，染色后根據每克濕重加入20ml抽提液（乙醇：二甲亞礬一50 ： 50 ） ，在密閉容器內避光置24h ，測量前搖勻后取200pi至96孔板，在490urn波長，酶標儀測定各孔od值。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。Results. the 120 specimens from 30 patients underwent bacterial culture growth : 116 were sterile, an 4 aerobic cultures ( 2 patients ) grew coagulase - negatie staphylococci, suggestie of contamination
結果：這來自30個病人的120片碎片在經歷了細菌培養程序后發現： 116片是無菌的，有4片（來自2個病人）在有氧條件下培養除了凝固酶陰性的葡萄球菌，提示被污染了。Anaerobic culture apparatus
無氧培養器In my experiment, only beta - mercaptoethanol have the potency to induce mscs to neural cells, exclude the other antioxidant such as glutathione, ascorbic acid and vitamin e ( alcohol dissolved ), and using beta - mercaptoethanol and serum - free dmem medium as the main differentiation a - gent
結果顯示，只有beta -巰基乙醇具備此種誘導能力，排除了谷胱甘肽、抗壞血酸、維生素e等抗氧化劑的作用。本實驗使用了beta -巰基乙醇、無血清的dmem培養液和dmso作為誘導方案，在誘導后，可見很多誘導產生的神經元樣和膠質細胞樣細胞。分享友人