無氮培養基 的英文怎麼說
中文拼音 [wúdànpéiyǎngjī]
無氮培養基
英文
nitrogen-free agar-
Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria
該菌在初始ph4 - 10的培養基中都能夠生長，生長最適溫度范圍為28 - 30 . 5 ，在pda 、查氏等真菌培養基中生長旺盛，在高氏1號和無氮源培養基中同樣生長良好，而在lb與牛肉膏蛋白腖等細菌培養基中生長很差，碳源是其生長的關鍵因子，這有別於一般細菌的營養需求。67 - 154. 02 % ( no determinate in paddy field on quaternary red clay ), respectively. after organic amendments and fertilizers were added to the metsulfuron - methyl - contaminated soils, microbiai biomass c increased by 0. 23 - 113. 14 % in paddy field on desalting muddy polder, 0. 30 - 46. 48 % in blue clayed paddy, and 1. 82 - 83. 76 % in paddy field on quaternary red clay, respectively, microbiai biomass n in correspoading soils by 4. 27 - 67. 87 %, 5. 43 - 58. 36 % and 5. 05 - 95. 40 %, respectively, and microbiai biomass p by 6. 03 - 139. 59 % 4. 09 - 141. 26 % ( no determinate in paddy field on quaternary red clay ), respectively
（ 4 ）添加有機、無機物質后，勞去津除草劑污染的三種土壤中微生物生物量碳、氮隨培養時間變化的趨勢基本一致，即0 ~ 7d微生物生物碳、氮降低，但第7d時，添加有機、無機物質的處理中微生物生物量碳、氮均高於僅加養去津的處理和空白對照; 7 ~ 14d微生物生物量碳、氮迅速增加; 14 ~ 42d又下降， 42d后變化較小。Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced
首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0Flexor digitorum profundus tendons of chickens wer e cultured in the presence of deoxyguanosine ( dgua ) for 5 days, then cryopreserv ed in liquid nitrogen ( 196 ) without affecting their viability
用脫氧鳥苷培養處理雞屈趾深肌腱5天，無創凍存於液氮貯存器（ 196 ）中3個月，使用前將凍存腱在40溶液中融解並洗去腱中吸收的二甲基亞碸。The best condition for extracting polysaccharide from porphyridium cruentum were as follow : alcohol concentration was 50 %, alcohol volume was 1 - fold time, percolation time was 0. 5h, the volume ratio of glycoprotein solution to sevag reagent was 2 : 1, time was 45min and sevag reagent was 4 : 1 between chloroform and butanol. the result also indicate that sodium acetate anhydrous and nh4cl were the best carbonic and nitrogen source for polysa
血色紫球藻的最優提取工藝為乙醇濃度50 % ，乙醇用量為1倍體積，醇沉時間為0 . 5小時;氯仿與正丁醇的比例4 : 1 ，樣液與sevag試劑的比例2 : 1 ，作用時間為45min ;五種碳源的影響差異不顯著，氮源的影響差異顯著，其中添加無水乙酸鈉和nh4ci的培養基多糖產率最高，分別為33 . 784mg / l和40 . 997mg / l 。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。Uniform design was employed to optimize the culture conditions and the component of culture medium including carbon source, nitrogen source, phosphor source, growth factors, inorganic salts and precusor as well
利用均勻設計原理進行實驗設計，以優化培養基中碳源、氮源、磷源、生長因子、前體物及無機鹽成份的配方以及細菌培養條件。In nitrogen - free medium, the logarithmic phase of the strain w12 was about 13 - 48 h. in logarithmic period its doubling time was 2 - 4 h in nitrogen - free medium, 0. 9 - lh when enough nk
在無氮培養基上該菌在13h后進入對數生長期，並持續至48 52h ，這期間其生長代時為2 - 4h ，有氮培養基上為0 . 9 - 1h 。分享友人