無清蛋白血 的英文怎麼說
中文拼音 [wúqīngdànbáixiě]
無清蛋白血
英文
analbuminemia- 無 : 無Ⅰ動詞(沒有) not have; there is not; be without Ⅱ名詞1 (沒有) nothing; nil 2 (姓氏) a surn...
- 清 : Ⅰ形容詞1 (純凈) unmixed; clear 2 (寂靜) quiet 3 (清楚) distinct; clarified 4 (一點不留) w...
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 血 : 血名詞(血液 多用於口語) blood:吐血 spit (up) blood; 血的教訓 a lesson paid for [written] in b...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture
提要:採用無血清無動物組分培養基馴化高效表達細胞,構建高效表達系統,懸浮培養細胞,可以在規模化生產中,提高細胞密度,增強細胞活力,控制細胞增殖水平,延長細胞培養周期,增加目標蛋白的產量,提高產品質量,簡化生產工藝,降低生產成本,進而提高規模化培養的效能。The functions of these cells include the formation and excretion of bile, regulation of carbohydrate homeostasis, lipid synthesis and secretion of plasma lipoproteins, control of cholesterol metabolism, formation of urea, serum albumin, clotting factors, enzymes and numerous proteins
這些細胞的功能包括膽汁的形成和分泌?碳水化合物穩定的調節?脂質的合成和血漿脂蛋白的分泌?膽固醇代謝的控制?尿素?血清白蛋白?凝血因子? ?以及無數種蛋白的形成。The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明:麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白,分子量分別為181kd和136kd ; sds - page電泳分析:存在3條雌特異性帶,其分子量為123kd 、 120kd和91kd ,由此,可推定卵黃原蛋白( vitellogenin , vg )和卵黃磷蛋白( vitellin , vn )由2個蛋白組成,其中分子量較大的蛋白由2個亞基組成;雙向電泳結果顯示,在120kd附近有兩個特異性點,其等電點為5 . 5和5 . 7 ;雙擴散表明,麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應,而與雄蜂血淋巴無免疫反應,說明了vg與vn具有免疫同源性,是雌特異性蛋白,且由脂肪體合成。A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity
報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑(溴化十二烷基三乙銨/十二烷基硫酸鈉)分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基,研究蛋白質在雙水相體系中的親和分配.結果表明,在該體系中,表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成,不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性New york ( reuters health ) jun 20 - the proportion of plasma cells in bone marrow and the serum monoclonal protein leel at diagnosis influence the risk that smoldering ( asymptomatic ) multiple myeloma will progress to symptomatic disease, new research suggests
紐約路透通訊社健康欄6月20日訊:最新研究發現,骨髓血漿細胞和血清單克隆蛋白水平的比例會影響無癥狀性多發性骨髓瘤發展為有癥狀性骨髓瘤的風險。Method to collect respectively 180 unrelated males " venous blood 500ul, who lived in shanxi province, 120 unrelated mongolians " venous blood 500ul, who lived in the inner mongolia autonomous region, and the blood is anticoagulant with edta, then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr
方法採集180例山西漢族和120例內蒙古蒙古族男性無關個體靜脈血各500ul , edta抗凝,用tkml液反復洗滌至無色,加入2蛋白酶k緩沖液180ul ,蛋白酶k ( 20mg ml ) 20ul ,在56消化至液體清亮為止,用酚-氯仿法抽提dna , pcr擴增dys413位點, 6非變性聚丙烯酰胺凝膠電泳, 1硝酸銀染色分型。Alpha - chain protein was not demonstrated in the patient ' s serum, urine, diluted duodenal juice, and jejunal biopsy tissue
但在病人的血清、尿液、十二指腸液、和空腸切片組織中無法顯示出甲種重鏈蛋白質。The biocomposite, which needs no additional curing, is directly used to construct the immunosensor at room temperature. the incorporation of bsa into the biocomposite reduces nonspecific adsorption of immunosensors to a lower level
生物組分在室溫下快速制備且無的孵化,在生物組分中加入一定量的牛血清白蛋白使免疫電極的4po異性吸附降低到一個很低的水平。A number of embryonic proteins are secreted by prostate tumours and can be detected in blood serum, thereby proiding a potential method for detecting prostate cancer and ultimately een assessing the extent of the disease without an inasie procedure
前列腺腫瘤分泌大量的胚胎蛋白並能在血清中檢測到,因此為檢測到前列腺癌提供了一種可能方式,最終可通過無創方式來評估疾病程度Development of insect cell culture media and serum - free media, establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper
摘要綜述了昆蟲細胞培養基的現狀和發展、無血清培養基的開發、昆蟲細胞系的建立以及昆蟲細胞在生物農藥和重組蛋白中的應用。A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions, which means the cells should have some anti - apoptosis property ; if not cultured i n suspension, the cells should also be able to grow in adherence
一株成功的工程細胞除了要求目的蛋白的表達量高外,還必須適應無血清培養基培養;必須具有對不利環境的抵抗能力,即抗凋亡能力;對于非直接懸浮培養的細胞,還必須具備在無血清培養條件下的貼壁能力。In addition, the change mechanism of the secondary structure under alkaline ph - independent perturbation is discussed and it is considered that a - hexlix and p sheet unfold and convert into 3 turn and random structure
根據譜峰確認及其歸屬指派結果,討論了堿性ph擾動下的牛血清白蛋白二級結構的變化機制,認為隨著ph增加,螺旋和片層向轉角和無規纏繞轉換。分享友人