無細菌的培養 的英文怎麼說
中文拼音 [wúxìjūndepéiyǎng]
無細菌的培養
英文
bacteria-free culture- 無 : 無Ⅰ動詞(沒有) not have; there is not; be without Ⅱ名詞1 (沒有) nothing; nil 2 (姓氏) a surn...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 的 : 4次方是 The fourth power of 2 is direction
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 細菌 : germ; bacterium (pl. bacteria); fungus (pl. fungi)
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Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria
該菌在初始ph4 - 10的培養基中都能夠生長,生長最適溫度范圍為28 - 30 . 5 ,在pda 、查氏等真菌培養基中生長旺盛,在高氏1號和無氮源培養基中同樣生長良好,而在lb與牛肉膏蛋白腖等細菌培養基中生長很差,碳源是其生長的關鍵因子,這有別於一般細菌的營養需求。These two cases had cloudy peritoneal fluid with high eosinophilic count without abdominal pain, and negative of peritoneal fluid culture and gram stain
這些病人臨床上的特徵有腹膜透析液混濁、嗜伊紅性白血球增高,但無腹痛且腹膜透析液的培養及革蘭氏細菌染色均正常。Results showed that in the water body of xizi lake, annual average of culturable planktonic ammonifiers and nitrogen fixers were 510 and 236 cfu / ml, respectively ; ammonia oxidizers, nitrite oxidizers, nitrate reducers and denitrifiers were 8. 5, 16, 587 and 16 mpn / ml, respectively ; inorganic phosphate solubilizing bacteria ( 1pb ) and organic phosphorus mineralizing bacteria ( opb ) were 89 cfu / ml and 37 mpn / ml, aerobic and anaerobic cellulose decomposers were 7 and 5 mpn / ml, respectively
水體中可培養異養細菌(氨化細菌)和固氮菌的年平均值分別為510和236cfu ml ,氨氧化細菌、亞硝酸氧化細菌、硝酸鹽還原菌和脫氮菌的數量分別為8 . 5 、 16 、 587和16mpn ml ;無機磷和有機磷分解菌分別為89cfu ml和37mpn ml ;好氧性纖維素分解菌和厭氧性纖維素分解菌只有7和5mpn ml 。Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究:無氮培養條件下,測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響,結果表明,在15 - 40下均能生長並表達固氮酶活性,其最適生長及固氮的溫度為25 - 35 ;能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮,當培養基中同時存在蔗糖和蘋果酸時,細菌生長和固氮活性最強;在偏酸和偏堿的條件下( ph4 . 5 - 8 . 5 )均能保持較強的生長勢和較高的固氮酶活性,並能通過調節自身代謝平衡並適應環境的酸、堿性變化,使培養液趨于中性:能耐受較高的滲透壓,培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大,當naci濃度升至2時,菌株的生長勢及固氮酶活性才有所下降:低濃度的鉸對其固氮酶活性影響不大,在0The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6
方法無菌條件下,從6月齡紐西蘭白兔的膝關節囊內剪取滑膜組織,採用組織塊培養法和酶消化法分離滑膜細胞。Exosomes derived from human intestinal epithelial cell ( iec ) could be released from apical and basolateral sides and expressed mhc class i molecules, cd26, cd63 in basal conditions besides mhc class ii in inflammatory conditions. the data showed exosomes might be a mechanism of oral tolerance. exosome - like vesicles isolated from rat iec line, which were pulsed by antigens, were shown to induce antigen - spec
模型的建立和exosomes的分離與純化我們利用一種c57bl / 6小鼠的3lllewis肺癌細胞系作模型,將3ll細胞皮下注射到c57bl / 6小鼠,待3周時無菌取出腫瘤組織,剪碎後用dmem加10 %的血清在37 , 5 %的czo中培養, 30小時后收集培養上清。Had the highest activities of xylanase and cmcase among all isolates. with rice straw as fermentation substrates, the maximum activities of xylanase and cmcase by a4 were 13. 54 u ? ml - 1, 0. 25 u ? ml - 1, respectively
第二部分對培養基底物及主要組分無細胞瘤胃液和酵母膏對厭氧真菌a4菌產酶的影響進行了研究。The reduced level of cell - free rumen fluid had no significant effect on xylanase production, but had significant effect on the cmcase activity. without cell - free rumen fluid, the high concentration level of yeast extract could improve xylanase and cmcase production. in the third section, crude enzymes produced by anaerobic fungus a4 was extracted, and their characteristics of the crude enzyme was also investigated
與基礎產酶培養基相比,降低培養基中無細胞瘤胃液濃度對厭氧真菌所產木聚糖酶的酶活及比活力無顯著影響( p 0 . 05 ) ,但對其所產的羧甲基纖維素酶的酶活及比活力有顯著影響( p 0 . 05 ) 。We can see a lot of new pictures of induced neuron - like cells, include neuron - like cells and glial - like cells, with the apparent nerve cells. material and methods rat bone marrow cell were collected, after sacrifice of a 3 months old wistar rat, from femurs and tibias by flushing the shaft with culture media ( dmem - 10 % fetal bovine serum ) using a syringe of 5ml
無菌條件下取股骨和胚骨的骨髓b人snd含10胎牛血清的dmem培養液1000rpm離心10分鐘,吹打b人兩個培養瓶,置人37 j coh飽和濕度條件下培養,隔日更換培養液,當細胞達到70融合時,傳代。Uniform design was employed to optimize the culture conditions and the component of culture medium including carbon source, nitrogen source, phosphor source, growth factors, inorganic salts and precusor as well
利用均勻設計原理進行實驗設計,以優化培養基中碳源、氮源、磷源、生長因子、前體物及無機鹽成份的配方以及細菌培養條件。Results. the 120 specimens from 30 patients underwent bacterial culture growth : 116 were sterile, an 4 aerobic cultures ( 2 patients ) grew coagulase - negatie staphylococci, suggestie of contamination
結果:這來自30個病人的120片碎片在經歷了細菌培養程序后發現: 116片是無菌的,有4片(來自2個病人)在有氧條件下培養除了凝固酶陰性的葡萄球菌,提示被污染了。The ammonia - oxidizers were increased from 2. 13 106 / g mlss to 6. 28 108 / g mlss, while the nitrite - oxidizers were increased from 2. 13 103 / g mlss to 6. 28 106 / g mlss. after a month, most of the heteotrophic bacteria were washed out from the system. they nitrifying bacteria were prevalent in the enrichment system and were around 99 % in total bacteria
選取富含硝化細菌的活性污泥作為富集培養的對象,採用純無機培養基對硝化細菌進行定向富集培養,能在較短的時間內得到大量硝化細菌富集培養物,硝化細菌數約占總菌數的99以上。The symptoms of the nonbacterial prostatitis are similar to the chronic bacterial prostatitis, only the numbers of the leucocyte in the prostate liquid increase but have any bacterial or mycoplasma and chlamydia and other pathogenic microbe found
臨床醫生將癥狀像慢性前列腺炎前列腺液中有白細胞增多,但塗片及培養都沒有細菌,尿液檢查也沒有細菌的這類病例命名為無菌性前列腺炎。分享友人