無血清培養 的英文怎麼說

中文拼音 [wúxiěqīngpéiyǎng]
無血清培養 英文
serum-free culture
  • 無 : 無Ⅰ動詞（沒有） not have; there is not; be without Ⅱ名詞1 （沒有） nothing; nil 2 （姓氏） a surn...
  • 血 : 血名詞（血液 多用於口語） blood:吐血 spit （up） blood; 血的教訓 a lesson paid for [written] in b...
  • 清 : Ⅰ形容詞1 （純凈） unmixed; clear 2 （寂靜） quiet 3 （清楚） distinct; clarified 4 （一點不留） w...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 血清 : [免疫學] serum; blood serum血清病 serum sickness
  1. Subconfluent cultures of rat were maintained in dmem / 10 % fbs, 24hrs before neuronal induction, media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ), to initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media, 5hrs later, 40ul dmso was given to every hole containing 2ml each

    分別取第5代和第13代的mscs ，以8x10 cm 『濃度接種於六孔板中的蓋玻片上，制備細胞爬片，每孔加zinl培養液。達到80融合時，更換新鮮培養液，並在培養液中加人終濃度為lrnm的p一流基乙醇，誘導24小時， pbs洗滌，而後換成無血清的培養液。
  2. Using the mouse fetal ovary serum - free culture model, fetal ovaries from 14 day post coitus ( 14 dpc ) mouse were cultured, and treated by ay9944 - a - 7, nystatin and rs - 21745. the results showed that 0. 025, 0. 0625 and 0. 125 um ay9944 - a - 7 or 25, 50 and 75 iu / ml nystatin increased the total number of follicles per ovary significantly ; however, ay9944 - a - 7 and nystatin at the same doses could n ' t cause the same effect on the number of growing follicles and the average diameter of five largest follicles per ovary. 50 u. m rs - 21745 decreased the total number of follicles, the number of growing follicles and diameter of follicles per ovary significantly after 48 h

    首先利用小鼠胚胎卵巢的體外無血清培養模型，培養妊娠14天（ 14daypost - coitus ， 14dpc ）小鼠胚胎卵巢，分別添加能促進mas積累的ay9944 ，制黴菌素，和能抑制mas產生的rs - 21745進行處理，結果表明： 0 . 025 、 0 . 0625利0 . 125 m的ay9944 - a - 7與25 、 50和75iu ml的制黴菌素能顯著提高卵巢中形成卵泡的總數量，但是對生長卵泡數和卵泡直徑的作用不同；而mas合成抑制劑rs - 21745能夠顯著降低形成卵泡的總數量。
  3. Methods : cell culture in serum - free medium, indirect immunofluorescence cytochemistry were used

    方法：採用無血清細胞培養技術，間接免疫細胞化學染色法。
  4. The concentration of fbs ( fetal bovine serum ) and ncs ( newborn calf serum ) was influential on the culture process of mef ( mouse embryonic fibroblast ) and es cells

    血清濃度的高低對胎鼠成纖維細胞和es細胞的培養過程是有影響的。無血清或低濃度的血清能抑制細胞分裂增殖，使細胞生長出現停滯。
  5. Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture

    提要：採用無血清無動物組分培養基馴化高效表達細胞，構建高效表達系統，懸浮培養細胞，可以在規模化生產中，提高細胞密度，增強細胞活力，控制細胞增殖水平，延長細胞培養周期，增加目標蛋白的產量，提高產品質量，簡化生產工藝，降低生產成本，進而提高規模化培養的效能。
  6. Exosomes derived from human intestinal epithelial cell ( iec ) could be released from apical and basolateral sides and expressed mhc class i molecules, cd26, cd63 in basal conditions besides mhc class ii in inflammatory conditions. the data showed exosomes might be a mechanism of oral tolerance. exosome - like vesicles isolated from rat iec line, which were pulsed by antigens, were shown to induce antigen - spec

    模型的建立和exosomes的分離與純化我們利用一種c57bl / 6小鼠的3lllewis肺癌細胞系作模型，將3ll細胞皮下注射到c57bl / 6小鼠，待3周時無菌取出腫瘤組織，剪碎後用dmem加10 %的血清在37 ， 5 %的czo中培養， 30小時后收集培養上清。
  7. Conclusion this is a serum - free culture system for developing a conjunctival epithelial explants with improved purify and proliferative, which are important for the basic research and tissue engineer of conjunctival epithelial cells

    結論無血清培養系統的組織塊培養法可為結膜上皮細胞的疾病、藥理毒理研究及培養移植的結膜上皮細胞提供更為安全有效的細胞模型。
  8. Objective to investigate the culturing technique for conjunctival epithelial cells and to evaluate the facibility of serum - free culture system on culturing conjunctival epithelial explants

    摘要目的研究結膜上皮細胞體外培養的方法，並進一步探索無血清培養系統用於結膜上皮細胞培養的可行性。
  9. Results the epithelial cells cultured in serum - free medium not only demonstrated a significant increase in proliferative, colony - forming efficiency and cell generations, but also have the normal structural and functional properties than those in serum - containing medium

    結果無血清培養系統中結膜上皮細胞的增殖、克隆形成及細胞傳代能力均較含血清培養系統高，而且具備結膜上皮細胞全部組織結構及功能特點。
  10. Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells

    方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。
  11. This peptide is derived from proteolytic processing of the amyloid precursor protein ( app ). an increasing ' amount of experiments show that a p can induce various toxic reaction in brain, thus play a causal role in the pathogenesis of alzheimer ' s disease ( ad ). in the meantime, the incapability of neurons to regenerate in ad patients means that it may inhibit the proliferation, differentiation and even survival of nscs

    3 .體外培養14天後，取部分細胞，加入不含bfgf僅含egf （ 20n留ml ）的無血清培養基，分為三組:對照組; ap25一35毒性組加入20umol /的凝聚態a目25一35 :雌激素保護組同時加入zoumolz的凝聚態ap25一35和10一7mo比17一p雌二醇。
  12. They used this method to improve the surface lubricity, antithrombogenicity, antibacterial, nettability and cell growth activity of the biomaterials, get a series of photo - chemical immobilized biomaterials which had good biocompatibility

    接著在這一新材料表面對鼠成纖維細胞（ sto ）進行無血清培養，培養2小時后，在低於lcst的溫度下對sto細胞進行選擇性脫附。
  13. Development of insect cell culture media and serum - free media, establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper

    摘要綜述了昆蟲細胞培養基的現狀和發展、無血清培養基的開發、昆蟲細胞系的建立以及昆蟲細胞在生物農藥和重組蛋白中的應用。
  14. Experimental condition optimization study on inducing mouse embryonic stem cells differentiation into insulin producing cells by serum - free culture in vitro

    無血清培養法誘導小鼠胚胎幹細胞分化為胰島素分泌細胞的實驗條件優化選擇
  15. Study on serum - free medium for 5b1 cell growth

    1細胞生長的無血清培養基的研究
  16. To initiate induction, undifferentiated escs were dissociated and replanted on none - coated tissue culture plastic dishes in nsc medium. the medium was changed ever - y other day into serum - free nsc medium

    誘導分化時去飼養層細胞，重新接種于無包被的培養皿中，使用nsc培養液，逐漸更換為無血清nsc培養液。
  17. 34 we further investigate the apoptosis related gene. using different concentration of il - 16 treat jurkat cells in 1640 without serum, the expression level of bid and c - myc increase together with the increase il - 16 concentration. these suggest that bid and c - myc may take a part in the apoptosis induced by high concentration of il - 16

    結果表明，在無血清培養jurkat細胞24hr后，隨著rhil ? 16濃度的增加， jurkat細胞的凋亡程度也增加，與之相應， bid和c一myc的表達水平也增加，這表示bdc m廠可能參與il叫6誘導的扣水ct細胞凋亡的調節。
  18. A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions, which means the cells should have some anti - apoptosis property ; if not cultured i n suspension, the cells should also be able to grow in adherence

    一株成功的工程細胞除了要求目的蛋白的表達量高外，還必須適應無血清培養基培養；必須具有對不利環境的抵抗能力，即抗凋亡能力；對于非直接懸浮培養的細胞，還必須具備在無血清培養條件下的貼壁能力。
  19. The stable expression cell line was selected. expression level of the line cultured in serum - free medium was 580iu 106 cell 24h. the product expressed was purified by zn

    篩選到的穩定表達株在無血清培養基的表達量為580iu 106細胞24h 。
  20. In my experiment, only beta - mercaptoethanol have the potency to induce mscs to neural cells, exclude the other antioxidant such as glutathione, ascorbic acid and vitamin e ( alcohol dissolved ), and using beta - mercaptoethanol and serum - free dmem medium as the main differentiation a - gent

    結果顯示，只有beta -巰基乙醇具備此種誘導能力，排除了谷胱甘肽、抗壞血酸、維生素e等抗氧化劑的作用。本實驗使用了beta -巰基乙醇、無血清的dmem培養液和dmso作為誘導方案，在誘導后，可見很多誘導產生的神經元樣和膠質細胞樣細胞。
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